breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
RA 321,260 A→T intergenic (+179/‑348) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
JC JC 1,995,620 IS5 (–) +4 bp intergenic (‑261/‑32) uvrY ← / → yecU DNA‑binding transcriptional activator UvrY/protein YecU
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,416,241 T→C S18G (AGC→GGC)  yjfN ← protease activator YjfN

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 354596 354689 94 127 [0] [46] 48 prpE/codB propionyl‑CoA synthetase/cytosine transporter
* * ÷ NC_000913 361657 362203 547 152 [0] [0] 140 [lacA]–[lacY] [lacA],[lacY]
* * ÷ NC_000913 3423849–3424234 3424492–3424239 6–644 48 [47] [47] 50 [rrlD] [rrlD]
* * ÷ NC_000913 3883285 3883552 268 48 [47] [47] 50 dnaA chromosomal replication initiator protein DnaA
* * ÷ NC_000913 4415874 4416032 159 49 [47] [47] 51 [aidB]–[yjfN] [aidB],[yjfN]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 19796 =NA (NA)151 (1.170) 86/580 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 361656 0 (0.000)coding (205/612 nt) lacA galactoside O‑acetyltransferase
* ? NC_000913 70062 =124 (0.960)118 (0.990)
+22 bp
73/538 0.2 52.2% intergenic (‑14/‑325) araB/araC ribulokinase/DNA‑binding transcriptional dual regulator AraC
?NC_000913 = 367520 110 (0.850)intergenic (‑10/+67) lacI/mhpR DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR
* ? NC_000913 = 71265155 (1.200)126 (0.970) 60/582 0.7 61.9% coding (879/879 nt) araC DNA‑binding transcriptional dual regulator AraC
?NC_000913 = 354595 0 (0.000)intergenic (+3/‑327) prpE/codB propionyl‑CoA synthetase/cytosine transporter
* ? NC_000913 = 258675NA (NA)139 (1.070) 94/582 0.0 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 0 (0.000)coding (976/1254 nt) lacY lactose permease
* ? NC_000913 = 120780584 (0.650)45 (0.370) 31/550 2.8 31.1% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 120 (0.980)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)179 (1.400) 102/574 0.0 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein