Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_012971	4,302,992	(GCC)2→1	coding (408‑410/1338 nt)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase AmiB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_012971	4302990 =	1 (0.010)	50 (0.530)	39/580	1.1	99.0%	coding (406/1338 nt)	amiB	N‑acetylmuramoyl‑L‑alanine amidase AmiB
	?	NC_012971	= 4302986	0 (0.000)					coding (402/1338 nt)	amiB	N‑acetylmuramoyl‑L‑alanine amidase AmiB

GGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACAATGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGCACTTTGCTCAATGACGATCC  >  NC_012971/4302692‑4303285                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                        ggCGCGGATAACGTTGGGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTTGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCATCGTCAATAATCTGTTGAATGCGATTCGCTCTGGAACGCCTAAAGATGCACAGACGCTACGGCTGGTGTTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACAATGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTccgcc                                                                                                                                                                                                                                                                                                        <  2:433832/300‑1 (MQ=255)         ggataacgttgagttttatttgcgatcctgattatgcgtttagccatcaaagcaaacgcaccttggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaatgcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTgg                                                                                                                                                                                                                                                                                                >  2:353474‑M1/291‑300 (MQ=255)         ggataacgttgagttttattggcgatcctgattatgcgtttagccatcaaagcaaacgcaccgtggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaacgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgcagcaaccgcctcctCCGACCGt                                                                                                                                                                                                                                                                                                  <  2:521071‑M1/8‑1 (MQ=255)              acgttgagttttattggcgatcctgattatgcgtttagccatcaaagcaaacgcaccgtggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGc                                                                                                                                                                                                                                                                                            <  1:563048‑M1/14‑1 (MQ=255)                     gttttattggcgatcctgattatgcgtttagccatcaaagcaaacgcaccgtggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAaacac                                                                                                                                                                                                                                                                                     <  2:769226‑M1/21‑3 (MQ=255)                            ttgcgatcctgattatgcgtttagccatcaaagcaaacgcaccgtggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAAc                                                                                                                                                                                                                                                                             >  1:793649‑M1/272‑300 (MQ=255)                                     tgattatgcgtttagctatcaaagcaaacgcaccgtggcgctcgatatcaaacaaacgggcttgagtcagggacggccgttgttgttcagcggcaataatctggtgaagtcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagctgcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCgg                                                                                                                                                                                                                                                                     >  2:477465‑M1/263‑299 (MQ=255)                                     tgattatgcgtttagccatcaaagcaaacgcaccgtggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctcttgaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCgg                                                                                                                                                                                                                                                                     >  2:175277‑M1/263‑299 (MQ=255)                                      gattatgcgtttagccatcaaagcaaacgcaccgtggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGtt                                                                                                                                                                                                                                                                   <  1:252922‑M1/39‑1 (MQ=255)                                              gtttagccatcaaagcaaacgcaccttggcgctcgatatcaaacaaacgggcgtgattcatggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttatgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCAc                                                                                                                                                                                                                                                            >  2:466204‑M1/254‑299 (MQ=255)                                              gtttagccatcaaagcaaacgcaccgtggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCAc                                                                                                                                                                                                                                                            >  1:538292‑M1/254‑299 (MQ=255)                                                              aaacgcaccgtggcgctcgatatcaaacaaacgggcgtgattcatggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagnggtgaagcggcagaatggcagcaattacaatgncgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAAcc                                                                                                                                                                                                                                            >  2:608226‑M1/238‑299 (MQ=255)                                                                acgcaccgtggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattancgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGgc                                                                                                                                                                                                                                         <  2:638325‑M1/65‑1 (MQ=255)                                                                         ggcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATcc                                                                                                                                                                                                                                >  1:122762‑M1/227‑300 (MQ=255)                                                                          gcgctcgatatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATcc                                                                                                                                                                                                                                <  2:666190‑M1/74‑1 (MQ=255)                                                                                 atatcaaacaaacgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctaaggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaacgcctcctCCGACCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGAACCGCGCGTCAGCGAACCGGCGCGCAATCCGTCTaaaa                                                                                                                                                                                                                        <  2:213250‑M1/82‑1 (MQ=255)                                                                                      aaacaaatgggcgtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAGCGGaa                                                                                                                                                                                                                   <  2:289402‑M1/87‑1 (MQ=255)                                                                                               ggcgtgattcaggtactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCg                                                                                                                                                                                                           >  2:54826‑M1/205‑299 (MQ=255)                                                                                                  gtgattcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCAc                                                                                                                                                                                                        >  1:431775‑M1/202‑299 (MQ=255)                                                                                                     attcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttcccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTa                                                                                                                                                                                                      <  2:702266‑M1/100‑1 (MQ=255)                                                                                                     attcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttcccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACAGCACTAc                                                                                                                                                                                                     <  2:702254‑M1/101‑1 (MQ=255)                                                                                                     attcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTAc                                                                                                                                                                                                     <  2:794163‑M1/101‑1 (MQ=255)                                                                                                       tcagggactgccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacnctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACggg                                                                                                                                                                                                  >  1:169373‑M1/197‑300 (MQ=255)                                                                                                               tgccgttgttgttcagcggcaataatctgttgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATc                                                                                                                                                                                           >  2:762817‑M1/189‑299 (MQ=255)                                                                                                                 ccgttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctactCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAAACGCACTAAGGGTGTTATca                                                                                                                                                                                          <  2:672519‑M1/112‑1 (MQ=255)                                                                                                                   gttgttgttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATcagc                                                                                                                                                                                        >  1:698177‑M1/185‑298 (MQ=255)                                                                                                                         gttcagcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATAc                                                                                                                                                                                >  1:101359‑M1/179‑300 (MQ=255)                                                                                                                              gcggcaataatctggtgaaggcgattcgctctggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGTGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTaa                                                                                                                                                                           >  2:792871‑M1/174‑300 (MQ=255)                                                                                                                                 gcaataatctggtgaaggcgattcgctctggaacgcctaaagatggacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAAcgc                                                                                                                                                                        >  1:185601‑M1/171‑300 (MQ=255)                                                                                                                                                             ttgaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAacac                                                                                                                                            >  2:347480‑M1/143‑300 (MQ=255)                                                                                                                                                             tggaacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAacac                                                                                                                                            >  1:55326‑M1/143‑300 (MQ=255)                                                                                                                                                                aacgcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTgg                                                                                                                                         >  2:689041‑M1/140‑300 (MQ=255)                                                                                                                                                                   gcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGc                                                                                                                                        <  2:333332‑M1/162‑1 (MQ=255)                                                                                                                                                                   gcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGa                                                                                                                                      <  1:622377‑M1/164‑1 (MQ=255)                                                                                                                                                                   gcctaaagatgcacaaacgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctactCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGa                                                                                                                                      <  1:393295‑M1/164‑1 (MQ=255)                                                                                                                                                                   gcctaaagatgcacaaacgctacggctggtggtcgatcttaacgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCCCCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCCGCGCGCGTGACGGCTAACACTAGCGa                                                                                                                                      <  2:210069‑M1/164‑1 (MQ=255)                                                                                                                                                                                   acgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtggcgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCCGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCt                                                                                                                      <  2:487127‑M1/180‑1 (MQ=255)                                                                                                                                                                                   acgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCt                                                                                                                      <  1:699873‑M1/180‑1 (MQ=255)                                                                                                                                                                                    cgctacggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTa                                                                                                                     <  2:673644‑M1/181‑1 (MQ=255)                                                                                                                                                                                        acggctggtggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGa                                                                                                                 <  1:467285‑M1/185‑1 (MQ=255)                                                                                                                                                                                                tggtcgatcttaccgaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGac                                                                                                         >  1:306943‑M1/108‑300 (MQ=255)                                                                                                                                                                                                              gaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGAc                                                                                           >  1:37879‑M1/94‑300 (MQ=255)                                                                                                                                                                                                              gaaaacggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGAc                                                                                           >  2:673507‑M1/94‑300 (MQ=255)                                                                                                                                                                                                                    ggtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTgg                                                                                      >  1:128935‑M1/88‑299 (MQ=255)                                                                                                                                                                                                                     gtaaaaccgaagcggtgaagcggcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGgcg                                                                                    >  1:467283‑M1/87‑300 (MQ=255)                                                                                                                                                                                                                          accgacgcggtgaagcggcagaatggcagcaattacaatttcgtcgttacgattaacgccgatgtgacgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTTTCGCACCGCGCGTCGGCGAACCGGCGCGCAATCCGTTTAAAACTGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATc                                                                               >  2:493120‑M1/82‑300 (MQ=255)                                                                                                                                                                                                                                           gcagaatggcagcaattacaatgtcgtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGNAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGTTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTAcgc                                                               >  2:627730‑M1/65‑299 (MQ=255)                                                                                                                                                                                                                                                          ttacaatgtagtctttacgattaacgccgatgtgccgccaccgcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTANCATCGATATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAc                                               <  1:165959‑M1/251‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    gcctcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGCACTTTGCTCAATGACGa     >  2:479310‑M1/8‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       tcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTAAGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGATATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGCACTTTGCTCAATGACGATc   <  1:787838‑M1/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       tcctCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCACGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTAAGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGAAGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAACATCGCCATCGCGCGTAAATTGCGCACTTTGCTCAAtgacgctcc  <  2:29221‑M1/296‑5 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                        GGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACAATGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGCACTTTGCTCAATGACGATCC  >  NC_012971/4302692‑4303285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.