Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 949,906 IS2 (+) +5 bp coding (239‑243/591 nt) ycaK → putative oxidoreductase YcaK

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 949906 =0 (0.000)8 (0.730) 8/310 0.2 100% coding (239/591 nt) ycaK putative oxidoreductase YcaK
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 9499100 (0.000)11 (1.000) 10/310 0.1 100% coding (243/591 nt) ycaK putative oxidoreductase YcaK
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region

ACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGC  >  NC_000913/1469000‑1469423
                                                                                                                                                                                                                                                |                                                                                                                                                                                       
aCGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGa                                                                                                                                                                                >  1:126892/1‑250 (MQ=32)
                        tgctACACCATGTTGTCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTACTTCGGCCCTAAGACATCAATCATCTGGTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTGATTTAAGTGATATTGGTTGTCTGGAAATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGc                                                                                                                                                        <  1:270659/250‑1 (MQ=255)
                                  tGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAACGCTCTGCTGAACAATTGCGATCTTTTACTGTGAGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAACAACTAGTACTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGAGCGAAACTTGtt                                                                                                                                              >  1:263627/1‑250 (MQ=14)
                                       ccGGGCAGCGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTg                                                                                                                                                <  1:39891/243‑1 (MQ=255)
                                                                                                                                                                aTGACTAGTCTAAAAACTAGTATTAAGACTTTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGgaa               <  1:152400/251‑1 (MQ=255)
                                                                                                                                                                               aCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTta                                                                                                                                             <  1:3019/110‑1 (MQ=255)
                                                                                                                                                                                   gTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCCAAGAAATTACTTCTGCTGCTTACGGTCCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTGAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAg                                                                             >  1:219393/1‑170 (MQ=25)
                                                                                                                                                                                                                            gagaTTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCACCGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGc  >  1:106216/1‑204 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                       
ACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGC  >  NC_000913/1469000‑1469423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.