breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 4,116 C→T T128I (ACC→ATC)  thrC → threonine synthase
RA 10,659 C→T S233N (AGC→AAC)  yaaW ← putative enzyme‑specific chaperone YaaW
RA 18,600 C→T S371F (TCT→TTT)  nhaA → Na(+):H(+) antiporter NhaA
RA 25,099 C→T R903R (CGC→CGT ileS → isoleucine‑‑tRNA ligase
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
RA 224,625 T→A noncoding (855/1542 nt) rrsH → 16S ribosomal RNA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 366,022 C→G G95A (GGT→GCT)  lacZ ← beta‑galactosidase
RA 366,117 C→T W63* (TGG→TGA lacZ ← beta‑galactosidase
RA 656,315 A→C intergenic (‑347/‑242) dcuC ← / → pagP anaerobic C4‑dicarboxylate transporter DcuC/Lipid A palmitoyltransferase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,043,607 G→A L202L (CTG→TTG)  etk ← protein‑tyrosine kinase Etk
RA 1,050,092 C→T S87L (TCA→TTA) 
L22L (CTC→CTT
insA4 →
insB4 →
IS1 family protein InsA
IS1 family protein InsB
RA 1,146,079 C→A V173F (GTT→TTT)  yceF ← m(7)GTP pyrophosphatase
RA 1,157,902 G→T V12F (GTC→TTC)  ptsG → glucose‑specific PTS enzyme IIBC component
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,196,325 A→G K401K (AAA→AAG icd → isocitrate dehydrogenase
JC JC 1,219,391 IS1 (–) +9 bp intergenic (+190/+2906) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,220,493 C→T intergenic (+1292/+1812) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,233,669 G→A S350F (TCT→TTT)  nhaB ← Na(+):H(+) antiporter NhaB
RA 1,290,947 C→T Q236* (CAA→TAA)  rssB → regulator of RpoS
RA 1,292,992 A→C intergenic (‑70/‑535) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,374,498 G→T G137C (GGC→TGC)  ycjP → putative ABC transporter membrane subunit YcjP
RA 1,531,689 A→G intergenic (‑1185/‑127) yncO ← / → ydcC protein YncO/H repeat‑associated putative transposase YdcC
RA 1,553,630 C→A G70V (GGT→GTT)  adhP ← ethanol dehydrogenase/alcohol dehydrogenase
RA 1,555,731 Δ1 bp intergenic (‑62/+95) maeA ← / ← sra malate dehydrogenase (oxaloacetate‑decarboxylating)/ribosome‑associated protein Sra
RA 1,600,234 C→T intergenic (‑25/+54) lsrK ← / ← lsrR autoinducer‑2 kinase/DNA‑binding transcriptional repressor LsrR
RA 1,601,098 C→A S48S (TCG→TCT lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,601,103 C→T V47M (GTG→ATG)  lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
JC 1,738,830 Δ19 bp coding (987‑1005/1026 nt) purR → DNA‑binding transcriptional repressor PurR
RA 1,793,377 Δ1 bp coding (182/750 nt) btuD ← vitamin B12 ABC transporter ATP binding subunit
RA 1,806,073 A→G L4S (TTG→TCG)  ydiY ← acid‑inducible putative outer membrane protein YdiY
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,953,295 G→A K198K (AAG→AAA cmoA → carboxy‑S‑adenosyl‑L‑methionine synthase
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,084,054 G→A Q433Q (CAG→CAA sbcB → exodeoxyribonuclease I
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,326,483 C→T L125L (CTC→CTT atoB → acetyl‑CoA acetyltransferase
RA 2,348,366 C→A T328K (ACG→AAG)  nrdB → ribonucleoside‑diphosphate reductase 1 subunit beta
RA 2,373,902 C→T E368K (GAG→AAG)  menE ← o‑succinylbenzoate‑‑CoA ligase
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,387,701 G→A intergenic (+264/+9) yfbM → / ← yfbN DUF1877 domain‑containing protein YfbM/uncharacterized protein YfbN
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,411,821 G→A A90V (GCC→GTC)  hxpA ← hexitol phosphatase A
RA 2,423,678 G→A intergenic (‑139/+58) rpnB ← / ← hisP recombination‑promoting nuclease RpnB/lysine/arginine/ornithine ABC transporter/histidine ABC transporter, ATP binding subunit
RA 2,445,509 G→A L18F (CTC→TTC)  yfcA ← putative transporter YfcA
JC JC 2,456,094 IS1 (–) +9 bp intergenic (‑448/+225) yfcV ← / ← sixA putative fimbrial protein YfcV/phosphohistidine phosphatase SixA
RA 2,464,491 G→A L173F (CTT→TTT)  mlaA ← intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,489,528 G→A A220V (GCT→GTT)  yfdV ← putative transport protein YfdV
RA 2,506,941 G→A A318A (GCC→GCT fryC ← putative PTS enzyme IIC component FryC
RA 2,559,427 G→A R190R (AGG→AGA intZ → putative phage integrase IntZ
RA 2,564,496 G→T intergenic (+124/‑27) yffR → / → yffS uncharacterized protein YffR/uncharacterized protein YffS
RA 2,589,494 A→G S634G (AGC→GGC)  acrD → multidrug efflux pump RND permease AcrD
RA 2,608,658 G→A G58S (GGC→AGC)  hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,625,695 C→T A194V (GCA→GTA)  ppx → exopolyphosphatase
RA 2,640,130 G→A A149V (GCC→GTC)  hisS ← histidine‑‑tRNA ligase
RA 2,640,445 G→A P44L (CCG→CTG)  hisS ← histidine‑‑tRNA ligase
RA 2,678,665 C→T A235T (GCG→ACG)  yphF ← putative ABC transporter periplasmic binding protein YphF
RA 2,681,333 C→T E471E (GAG→GAA yphG ← DUF5107 domain‑containing protein YphG
RA 2,684,810 C→T G233D (GGT→GAT)  glyA ← serine hydroxymethyltransferase
RA 2,702,887 G→A A167V (GCA→GTA)  era ← 30S ribosomal subunit maturation GTPase Era
RA 2,717,705 G→A T275I (ACT→ATT)  yfiF ← putative methyltransferase YfiF
RA 2,732,992 G→A I394I (ATC→ATT
I394I (ATC→ATT
clpB ←
clpB ←
chaperone protein ClpB
chaperone protein ClpB
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 3,040,842 2 bp→AA coding (228‑229/267 nt) sdhE ← FAD assembly factor
RA 3,043,360 G→A I88I (ATC→ATT yqfB ← N(4)‑acetylcytidine amidohydrolase
RA 3,071,412 C→T intergenic (‑168/+47) fbaA ← / ← pgk fructose‑bisphosphate aldolase class II/phosphoglycerate kinase
RA 3,089,109 A→T K276* (AAA→TAA)  galP → galactose:H(+) symporter
RA 3,132,532 G→A P227P (CCC→CCT yghR ← putative ATP‑binding protein YghR
RA 3,167,566 C→T V51V (GTG→GTA ygiS ← putative deoxycholate binding periplasmic protein
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
MC JC 3,349,206 Δ16,350 bp IS5‑mediated [mtgA][yhcE] [mtgA], elbB, arcZ, arcB, yhcC, gltB, gltD, gltF, yhcA, yhcD, [yhcE]
RA 3,373,606 A→G intergenic (‑30/+92) nanA ← / ← nanR N‑acetylneuraminate lyase/NanR
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,428,918 Δ1 bp intergenic (‑156/‑318) rrsD ← / → yrdA 16S ribosomal RNA/hexapeptide repeat‑containing protein YrdA
RA 3,428,923 Δ1 bp intergenic (‑161/‑313) rrsD ← / → yrdA 16S ribosomal RNA/hexapeptide repeat‑containing protein YrdA
RA 3,428,927 T→G intergenic (‑165/‑309) rrsD ← / → yrdA 16S ribosomal RNA/hexapeptide repeat‑containing protein YrdA
RA 3,432,105 C→T L120L (CTG→CTA smg ← DUF494 domain‑containing protein Smg
RA 3,441,421 G→T N85K (AAC→AAA rpsD ← 30S ribosomal subunit protein S4
RA 3,443,961 G→A A46V (GCC→GTC)  secY ← Sec translocon subunit SecY
RA 3,443,980 G→A P40S (CCG→TCG)  secY ← Sec translocon subunit SecY
RA 3,452,022 G→A P89S (CCG→TCG)  rplD ← 50S ribosomal subunit protein L4
RA 3,452,218 G→A F23F (TTC→TTT rplD ← 50S ribosomal subunit protein L4
RA 3,457,378 C→T L334L (CTC→CTT gspD → Type II secretion system protein GspD
RA 3,458,245 A→C S623S (TCA→TCC gspD → Type II secretion system protein GspD
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,494,679 C→T T223T (ACC→ACT nirB → nitrite reductase (NADH) large subunit
RA 3,525,566 T→C intergenic (+143/+23) mrcA → / ← nudE peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA/ADP‑sugar diphosphatase NudE
RA 3,537,999 C→T F205F (TTC→TTT yhgF → putative RNA‑binding protein YhgF
RA 3,555,047 2 bp→TC coding (1963‑1964/2706 nt) malT → DNA‑binding transcriptional activator MalT
RA 3,557,868 Δ1 bp coding (212/1227 nt) rtcB ← RNA‑splicing ligase
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,606,891 C→T F147F (TTC→TTT zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
RA 3,617,379 T→A I122K (ATA→AAA)  nikD → Ni(2(+)) ABC transporter ATP binding subunit NikD
RA 3,624,545 T→A D56E (GAT→GAA yhhI → putative transposase
RA 3,631,356 2 bp→AG coding (1234‑1235/1623 nt) yhiJ ← DUF4049 domain‑containing protein YhiJ
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,672,236 T→A intergenic (‑342/‑178) yhjB ← / → rcdB putative DNA‑binding transcriptional regulator YhjB/putative DNA‑binding transcriptional regulator YhjC
RA 3,679,508 C→T T30T (ACC→ACT kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,686,705 G→A F823F (TTC→TTT bcsC ← cellulose synthase outer membrane channel
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,712,407 G→A G176G (GGC→GGT yhjY ← putative outer membrane protein YhjY
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,955 2 bp→AA coding (810‑811/1323 nt) xylA ← xylose isomerase
JC JC 3,731,420 IS1 (–) +9 bp coding (290‑298/993 nt) xylF → xylose ABC transporter periplasmic binding protein
RA 3,769,447 G→A E35K (GAA→AAA)  yibV → PF15596 family protein YibV
RA 3,773,040 (G)6→4 coding (760‑761/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,783,983 G→A S49F (TCC→TTC)  secB ← protein export chaperone SecB
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,865,885 A→G V130A (GTT→GCT)  yidE ← putative transport protein YidE
RA 3,880,358 G→A F288F (TTC→TTT recF ← recombination mediator protein RecF
RA 3,899,195 C→T E67K (GAA→AAA)  yieK ← putative glucosamine‑6‑phosphate deaminase YieK
RA 3,903,234 G→A V156V (GTC→GTT bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,961,662 G→T G329V (GGC→GTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,965,174 G→A H153Y (CAC→TAC)  rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,968,683 G→A M256I (ATG→ATA rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,969,748 T→C S240P (TCG→CCG)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,999,664 C→T S561L (TCG→TTG)  uvrD → DNA helicase II
RA 4,009,698 G→A A177T (GCA→ACA)  pldB → lysophospholipase L2
RA 4,017,197 C→T intergenic (+5/‑136) udp → / → rmuC uridine phosphorylase/putative recombination limiting protein RmuC
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,108,758 T→C intergenic (+244/‑76) pfkA → / → sbp 6‑phosphofructokinase 1/sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,156,839 G→A intergenic (+50/‑11) argB → / → argH acetylglutamate kinase/argininosuccinate lyase
RA 4,157,116 Δ1 bp coding (267/1374 nt) argH → argininosuccinate lyase
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,202,259 G→A G112R (GGG→AGG)  zraS → sensor histidine kinase ZraS
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,400,254 C→T intergenic (+52/‑34) miaA → / → hfq tRNA dimethylallyltransferase/RNA‑binding protein Hfq
RA 4,423,337 C→T S164S (TCC→TCT ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE
RA 4,449,586 G→A P23S (CCG→TCG)  ppa ← inorganic pyrophosphatase
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,512,054 C→T A121T (GCG→ACG)  fecD ← ferric citrate ABC transporter membrane subunit FecD
RA 4,514,334 C→T intergenic (‑26/+19) fecB ← / ← fecA ferric citrate ABC transporter periplasmic binding protein/ferric citrate outer membrane transporter
RA 4,519,952 C→T G125R (GGG→AGG)  yjhU ← putative DNA‑binding transcriptional regulator YjhU
RA 4,525,476 C→T A110T (GCT→ACT)  yjhI ← putative DNA‑binding transcriptional regulator YjhI
RA 4,526,033 C→T intergenic (‑230/+73) yjhI ← / ← sgcR putative DNA‑binding transcriptional regulator YjhI/putative DNA‑binding transcriptional regulator SgcR
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,538,145 C→T W207* (TGG→TGA nanM ← N‑acetylneuraminate mutarotase
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,568,857 C→T intergenic (‑130/+1305) ytiE ← / ← yjiR protein YtiE/fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
RA 4,587,426 C→T L160L (CTG→TTG)  mrr → Type IV methyl‑directed restriction enzyme EcoKMrr
MC JC 4,593,901 Δ54 bp coding (769‑822/1362 nt) lgoT ← galactonate:H(+) symporter
RA 4,617,547 C→T N75N (AAC→AAT deoC → deoxyribose‑phosphate aldolase
RA 4,624,715 C→T A25A (GCG→GCA ytjB ← putative inner membrane protein Smp
RA 4,634,232 C→T L146L (CTG→TTG)  ytjC → putative phosphatase
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 583234 583691 458 10 [6] [8] 9 [appY] [appY]
* * ÷ NC_000913 1196375 1211456 15082 9 [2] [8] 9 C0293–mcrA C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1632712 1633489–1633251 540–778 9 [8] [8] 9 ynfT–ydfK ynfT,ydfK
* * ÷ NC_000913 2903699 2903868 170 9 [8] [6] 9 ygcE/queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
* * ÷ NC_000913 3423885–3424234 3424409–3424238 5–525 10 [8] [8] 9 rrlD 23S ribosomal RNA
* * ÷ NC_000913 3797089 3797410 322 10 [8] [8] 10 waaL O‑antigen ligase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)26 (0.240) 26/470 1.8 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein