breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
MC JC 575,786 Δ991 bp IS5‑mediated nmpC ← putative outer membrane porin NmpC
JC JC 577,688 IS3 (+) +3 bp coding (76‑78/498 nt) rrrD → lysozyme
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,301,917 C→T Q246* (CAG→TAG)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,889,802 G→T intergenic (‑56/+149) fadD ← / ← yeaY long‑chain‑fatty‑acid‑‑CoA ligase/Slp family lipoprotein YeaY
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
MC JC 2,102,944 Δ685 bp IS5‑mediated [wbbL][wbbK] [wbbL], [wbbK]
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
JC JC 2,698,402 IS30 (+) +2 bp coding (148‑149/636 nt) pgpC ← phosphatidylglycerophosphatase C
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,707,484 +GA :: IS3 (+) +4 bp coding (219‑222/1608 nt) dppA ← dipeptide ABC transporter periplasmic binding protein
JC JC 3,720,136 IS3 (–) +3 bp coding (88‑90/213 nt) cspA → cold shock protein CspA
JC JC 3,890,411 IS1 (–) +9 bp coding (176‑184/1248 nt) tnaB → tryptophan:H(+) symporter TnaB
JC JC 4,163,521 IS2 (–) +5 bp intergenic (‑251/‑114) trmA ← / → btuB tRNA m(5)U54 methyltransferase/cobalamin outer membrane transporter
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978495 1979270–1978503 9–776 123 [0] [0] 176 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423766–3424234 3424463–3424238 5–698 33 [31] [32] 33 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 273955 =NA (NA)122 (1.260) 73/598 0.1 47.7% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 800505 134 (1.380)coding (71/954 nt) ybhD putative DNA‑binding transcriptional regulator YbhD
* ? NC_000913 = 56677494 (0.970)81 (0.840) 31/594 2.2 44.8% pseudogene (91/93 nt) renD protein RenD
?NC_000913 568036 = 106 (1.100)pseudogene (2/213 nt) renD protein RenD
* ? NC_000913 = 689045NA (NA)83 (0.860) 56/598 0.5 38.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 720172 = 131 (1.350)coding (289/2199 nt) speF inducible ornithine decarboxylase
* ? NC_000913 1207790 =24 (0.250)66 (0.720) 48/566 0.7 72.6% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 27 (0.290)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780524 (0.250)66 (0.720) 46/566 0.8 72.6% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 27 (0.290)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)147 (1.540) 93/590 0.0 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)123 (1.270) 74/598 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1469296 =NA (NA)58 (0.600) 36/594 1.7 40.8% noncoding (1/1221 nt) IS30 repeat region
?NC_000913 = 1995730 84 (0.870)coding (76/177 nt) yecU protein YecU
* ? NC_000913 = 1470516NA (NA)56 (0.580) 38/598 1.5 39.7% noncoding (1221/1221 nt) IS30 repeat region
?NC_000913 1995729 = 85 (0.880)coding (75/177 nt) yecU protein YecU
* ? NC_000913 = 1651548NA (NA)35 (0.360) 30/594 2.3 21.1% noncoding (706/706 nt) IS2 repeat region
?NC_000913 1995543 = 131 (1.360)intergenic (‑184/‑112) uvrY/yecU DNA‑binding transcriptional activator UvrY/protein YecU
* ? NC_000913 1979271 =0 (0.000)176 (1.810) 106/598 0.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 1995547128 (1.320)34 (0.350) 28/598 2.6 21.0% intergenic (‑188/‑108) uvrY/yecU DNA‑binding transcriptional activator UvrY/protein YecU
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 3978530 =36 (0.370)64 (0.660) 49/598 0.8 64.0% coding (1006/1080 nt) wecF TDP‑N‑acetylfucosamine:lipid II N‑acetylfucosaminyltransferase
?NC_000913 4507459 = NA (NA)noncoding (1221/1221 nt) IS30 repeat region
* ? NC_000913 = 397853135 (0.360)46 (0.480) 34/594 1.9 56.8% coding (1007/1080 nt) wecF TDP‑N‑acetylfucosamine:lipid II N‑acetylfucosaminyltransferase
?NC_000913 = 4508679 NA (NA)noncoding (1/1221 nt) IS30 repeat region