breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 45 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913339,9790GA63.2% 25.9 / 37.3 38V71M (GTG→ATG) yahHputative uncharacterized protein YahH
*NC_000913339,9860GA63.2% 26.0 / 40.3 38R73Q (CGA→CAA) yahHputative uncharacterized protein YahH
*NC_0009134,361,1830CA42.3% 118.6 / 227.7 175S251I (AGC→ATC) cadCDNA‑binding transcriptional activator CadC
*NC_000913388,9670TC40.2% 54.2 / 118.0 92D254G (GAC→GGC) hemBporphobilinogen synthase
*NC_0009132,477,2650AC37.2% 146.7 / 37.7 94L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_000913339,9920C.36.8% 68.0 / 56.7 38coding (224/321 nt)yahHputative uncharacterized protein YahH
*NC_000913847,9550GA35.4% 163.8 / 49.0 127A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009131,351,4630CA34.6% 119.9 / 12.3 81E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009132,767,4450CA33.9% 228.6 / 31.5 123intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009133,911,5330CA32.9% 248.5 / 21.4 149intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009133,741,8710GT32.4% 186.2 / 15.2 108S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_0009133,054,8940GT32.0% 150.1 / 25.3 98P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009133,269,9430GT31.4% 99.4 / 15.4 70intergenic (+341/+273)yhaC/rnpBuncharacterized protein YhaC/RNase P catalytic RNA component
*NC_0009132,990,4800CA31.1% 326.9 / 29.4 180intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009131,922,8150CA31.0% 131.6 / 21.4 87G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_00091385,6510GT30.6% 166.8 / 12.6 112E8* (GAG→TAG) ilvIacetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
*NC_0009132,767,4440CA30.6% 254.1 / 10.7 124intergenic (+89/‑266)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009131,820,7630GT30.4% 173.5 / 10.1 93S151Y (TCC→TAC) chbCN,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
*NC_0009132,457,1770TG30.3% 168.5 / 16.8 91G661G (GGA→GGCfadJ3‑hydroxyacyl‑CoA dehydrogenase FadJ
*NC_0009132,767,4470CA30.1% 289.4 / 12.7 133intergenic (+92/‑263)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 265341979 (0.760)10 (0.110) 10/548 6.4 11.9% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 75 (0.790)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 69674169 (0.670)7 (0.090) 5/442 7.3 12.4% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 48 (0.630)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 2357664129 (1.250)10 (0.100) 8/576 7.5 7.6% coding (761/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357848 = 120 (1.200)intergenic (+45/+194) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 78122256 (0.540)4 (0.050) 4/446 8.0 7.6% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781653 = 56 (0.730)intergenic (+1/‑432) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 2063221108 (1.040)5 (0.050) 4/592 9.6 4.4% coding (103/933 nt) ldtA L,D‑transpeptidase LdtA
?NC_000913 2063238 = 108 (1.060)coding (86/933 nt) ldtA L,D‑transpeptidase LdtA
* ? NC_000913 = 61099582 (0.790)3 (0.030) 3/596 10.3 3.6% coding (1500/2241 nt) fepA ferric enterobactin outer membrane transporter
?NC_000913 611101 = 78 (0.760)coding (1394/2241 nt) fepA ferric enterobactin outer membrane transporter