Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,176,206	(CAGCACTTTGC)1→2	coding (116/1263 nt)	gatZ ←	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2176196 =	4 (0.140)	19 (0.680)	19/488	0.5	86.4%	coding (126/1263 nt)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
	?	NC_000913	= 2176206	2 (0.070)					coding (116/1263 nt)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

GGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCATTGCGGATTTAGCCGACTGCAAATAATCCCGGGGATCGGTCGCT  >  NC_000913/2175974‑2176449                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ggcacgaacatattcctttaccagctcgacggatttttccatcgccgcatccgcattttcttgctgccagcagtttggccccagatgatcgccgccgagaataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCt                                                                                                                                                                                                                                              >  1:76559‑M1/234‑251 (MQ=255) ggCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgct                                                                                                                                                                                                                                   >  1:76559‑M2/1‑233 (MQ=255)  gcacgaacatattcctttaccagctcgacggatttttccatcgccgcatccgcattttcttgctgccagcagtttggccccagatgatcgccgccgagaataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTg                                                                                                                                                                                                                                             >  1:266811‑M1/233‑251 (MQ=255)  gCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctg                                                                                                                                                                                                                                  >  1:266811‑M2/1‑232 (MQ=255)          atattcctttaccagctcgacggatttgtccatcgccgcatccgcattttcttgctgccagcagtttggccccagatgatcgccgccgataataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtcttccggtgtcattccggtatatcctccaaagtgattgacctggttttacgttggttcaatcagctctttgcCAGCACTTTGCTCGTTCTGTTTCGATc                                                                                                                                                                                                                                     >  1:101083‑M1/225‑251 (MQ=255)          atatTCCTTTACCAGCTCGACGGATTTGTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGATAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTTCCGGTGTCATTCCGGTATATCCTCCAAAGTGATTGACCTGGTTTTACGTTGGTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCTCTTTGCcagcactttgctcgttctgtttcgatc                                                                                                                                                                                                                          >  1:101083‑M2/1‑224 (MQ=255)                  ttaccagctcgacggatttttccatcgccgcatccgcattttcttgctgccagcagtttggccccagatgatcgccgccgagaataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGc                                                                                                                                                                                                                               >  2:113940‑M1/217‑249 (MQ=255)                  ttACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgc                                                                                                                                                                                                                    >  2:113940‑M2/1‑216 (MQ=255)                                              cgcatccgcattttcttgctgccagcagtttggccccagatgatcgccgccgagaataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGc                                                                                                                                                                                                 <  1:118672‑M1/63‑1 (MQ=255)                                              cgcatccgcatTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggc                                                                                                                                                                                      <  1:118672‑M2/251‑64 (MQ=255)                                                                              gccccagatgatcgccgccgagaataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCAAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCAc                                                                                                                                                                 <  2:110295‑M1/95‑1 (MQ=255)                                                                              gCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgcaagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcac                                                                                                                                                      <  2:110295‑M2/251‑96 (MQ=255)                                                                                           gccgccgagaataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCg                                                                                                                                                    >  2:19448‑M1/144‑251 (MQ=255)                                                                                           gccgccGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccg                                                                                                                                         >  2:19448‑M2/1‑143 (MQ=255)                                                                                                 gagaataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAAt                                                                                                                                              >  2:185903‑M1/138‑251 (MQ=255)                                                                                                 gagaATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaat                                                                                                                                   >  2:185903‑M2/1‑137 (MQ=255)                                                                                                     ataatgcgttcgcgtgcaaacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAAc                                                                                                                                          >  2:118871‑M1/134‑251 (MQ=255)                                                                                                     ataatGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaac                                                                                                                               >  2:118871‑M2/1‑133 (MQ=255)                                                                                                                       aacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCAt                                                                                                                                  <  1:250008‑M1/126‑1 (MQ=255)                                                                                                                       aacccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATGAACGTTTTCAt                                                                                                                                  >  2:250008‑M1/116‑241 (MQ=255)                                                                                                                       aaCCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaacgttttcat                                                                                                                       <  1:250008‑M2/241‑127 (MQ=255)                                                                                                                       aaCCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaatgaacgttttcat                                                                                                                       >  2:250008‑M2/1‑115 (MQ=255)                                                                                                                          ccaactttatcggcaatcgtaaaaacaaattcgcgaaagtctgccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGt                                                                                                                     <  1:113638‑M1/139‑1 (MQ=255)                                                                                                                          ccAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaacgttttcatgttttttccttgt                                                                                                          <  1:113638‑M2/251‑140 (MQ=255)                                                                                                                                                                    gccggtgtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAAt                                                                            >  2:242455‑M1/71‑250 (MQ=255)                                                                                                                                                                    gCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaacgttttcatgttttttccttgttaaatggcaggtgcgttatgccctgccctcgcagccacaat                                                                 >  2:242455‑M2/1‑70 (MQ=255)                                                                                                                                                                     cgggggtCATTCCGGTATAGCCTCCAAATTGAGTGACCTGGTTTGACGTTGCTTGAATCGGCACGTTGCCAGCACTTTGCGCGTGCGGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTTAACATATGCCGATATGGGCACCAGCTTTAGGCCGGGCAAGTAACTTTGTCAGGTTTTGTCCTTGTTAATGGGCAGGTGCGTTAAGCCCTTCTTTCGCAGCCTCAATCg                                                                          <  2:172670/246‑1 (MQ=255)                                                                                                                                                                          gtcattccggtataaccgccaaattgattgacctggtttgacgttgcttcactcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTCAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAAt                                                                     <  1:191155‑M1/187‑1 (MQ=255)                                                                                                                                                                          gtCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCACT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaacgttttcatgttttttccttgttcaatggcaggtgcgttatgccctgccctcgcagccacaatcggcaat                                                          <  1:191155‑M2/251‑188 (MQ=255)                                                                                                                                                                             attccggtataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGTGCAGAACAGACTGAACATATTCCTATATGTTCACCAGCTTTATGCCGGGCAATAAGCGTTTTCATGTTTTTTCCTTGTTAGATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCAc                                                                  >  1:272138‑M1/62‑251 (MQ=255)                                                                                                                                                                             aTTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgtgcagaacagactgaacatattcctatatgttcaccagctttatgccgggcaataagcgttttcatgttttttccttgttagatggcaggtgcgttatgccctgccctcgcagccacaatcggcaatcac                                                       >  1:272138‑M2/1‑61 (MQ=255)                                                                                                                                                                                     ataaccgccaaattgattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTCTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTc                                                           <  2:96252‑M1/197‑1 (MQ=255)                                                                                                                                                                                     ataACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaacgttctcatgttttttccttgttaaatggcaggtgcgttatgccctgccctcgcagccacaatcggcaatcactttgctc                                                <  2:96252‑M2/250‑198 (MQ=255)                                                                                                                                                                                                   gattgacctggtttgacgttgcttcaatcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCAt                                             >  2:49541‑M1/40‑250 (MQ=255)                                                                                                                                                                                                   gattgaCCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaacgttttcatgttttttccttgttaaatggcaggtgcgttatgccctgccctcgcagccacaatcggcaatcactttgctcaccacatcgcgcat                                  >  2:49541‑M2/1‑39 (MQ=255)                                                                                                                                                                                                                   tcatcacttagt‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAAACAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGAGCGTTATGCCCTGCCCTCGCAGCCCCAATCGGCAATCACTTTGCTCACCACATCTCGCAGTGCGGATTTAGCCGCCTGCAAATTATcc                 >  2:37700/12‑251 (MQ=255)                                                                                                                                                                                                                        cttcactcagcactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCATTGCGGATTTAGCCGACTGCaa                        <  1:196785‑M1/232‑1 (MQ=255)                                                                                                                                                                                                                        cTTCACT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCcagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaacgttttcatgttttttccttgttaaatggcaggtgcgttatgccctgccctcgcagccacaatcggcaatcactttgctcaccacatcgcgcattgcggatttagccgactgcaa             <  1:196785‑M2/250‑233 (MQ=255)                                                                                                                                                                                                                                  cactttgcCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCATTGCGGATTTAGCCGACTGCAAATAATCCCggg             >  2:54464‑M1/9‑251 (MQ=255)                                                                                                                                                                                                                                             cactttgccagcactttgcgcgtgctgttgcgatcaaatgccagcgccgcttcgataaccaacggatgggcagaacagactgaacatatgccgatatgttcaccagctttatgccgggcaattaacgttttcatgttttttccttgttaaatggcaggtgcgttatgccctgccctcgcagccacaatcggcaatcactttgctcaccacatcgcgcattgcggatttagccgactgcaaataatcccggg  >  2:54464‑M2/1‑8 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAAT‑‑‑‑‑‑‑‑‑‑‑CAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATATGTTCACCAGCTTTATGCCGGGCAATTAACGTTTTCATGTTTTTTCCTTGTTAAATGGCAGGTGCGTTATGCCCTGCCCTCGCAGCCACAATCGGCAATCACTTTGCTCACCACATCGCGCATTGCGGATTTAGCCGACTGCAAATAATCCCGGGGATCGGTCGCT  >  NC_000913/2175974‑2176449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.