Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,176,206	(CAGCACTTTGC)1→2	coding (116/1263 nt)	gatZ ←	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2176196 =	4 (0.140)	19 (0.680)	19/488	0.5	86.4%	coding (126/1263 nt)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
	?	NC_000913	= 2176206	2 (0.070)					coding (116/1263 nt)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

CCCGGGATTATTTGCAGTCGGCTAAATCCGCAATGCGCGATGTGGTGAGCAAAGTGATTGCCGATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATTTAACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2176438‑2176196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTCGGCGGCGATCATCTGGGGCCAAACTGCTGGCAGCAAGAAAATGCGGATGCGGCGATGGAAAAATCCGTCGAGCTGGTAAAGGAATATGTTCGTGCC  <  NC_000913/2176206‑2175974                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              CCCGGGATTATTTGCAGTCGGCTAAATCCGCAATGCGCGATGTGGTGAGCAAAGTGATTGCCGATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATTTAACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTG                                                                                                                                                                                                                                   >  2:54464/1‑251            TTGCAGTCGGCTAAATCCGCAATGCGCGATGTGGTGAGCAAAGTGATTGCCGATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATTTAACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGAGTGAAG                                                                                                                                                                                                                         <  1:196785/250‑1                                 ATGCGCGATGTGGTGAGCAAAGTGATTGCCGATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATTTAACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATC                                                                                                                                                                                                    >  2:49541/1‑250                                               GAGCAAAGTGATTGCCGATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATTTAACAAGGAAAAAACATGAGAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTAT                                                                                                                                                                                      <  2:96252/250‑1                                                      GTGATTGCCGATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATCTAACAAGGAAAAAACATGAAAACGCTTATTGCCCGGCATAAAGCTGGTGAACATATAGGAATATGTTCAGTCTGTTCTGCACATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAAT                                                                                                                                                                              >  1:272138/1‑251                                                         ATTGCCGATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATTGAACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGAGTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGAC                                                                                                                                                                           <  1:191155/251‑1                                                                ATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATTTAACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGC                                                                                                                                                                     >  2:242455/1‑250                                                                                                         ACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGG                                                                                                                           <  1:113638/251‑1                                                                                                                      ATGAAAACGTTCATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTT                                                                                                                        >  2:250008/1‑241                                                                                                                      ATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTT                                                                                                                        <  1:250008/241‑1                                                                                                                              GTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTAT                                                                                                      >  2:118871/1‑251                                                                                                                                  ATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTC                                                                                                  >  2:185903/1‑251                                                                                                                                        CGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTCGGCGGC                                                                                            >  2:19448/1‑251                                                                                                                                                     GTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTTGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTCGGCGGCGATCATCTGGGGC                                                                               <  2:110295/251‑1                                                                                                                                                                                     GCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTCGGCGGCGATCATCTGGGGCCAAACTGCTGGCAGCAAGAAAATGCGGATGCG                                               <  1:118672/251‑1                                                                                                                                                                                                                   GCATTTGATCGCAACAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTCGGCGGCGATCATCTGGGGCCAAACTGCTGGCAGCAAGAAAATGCGGATGCGGCGATGGAAAAATCCGTCGAGCTGGTAA                   >  2:113940/1‑249                                                                                                                                                                                                                         GATCGAAACAGAACGAGCAAAGTGCTGGCAAAGAGCTGATTGAACCAACGTAAAACCAGGTCAATCACTTTGGAGGATATACCGGAATGACACCGGAAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTATCGGCGGCGATCATCTGGGGCCAAACTGCTGGCAGCAAGAAAATGCGGATGCGGCGATGGACAAATCCGTCGAGCTGGTAAAGGAATAT           >  1:101083/1‑251                                                                                                                                                                                                                                 CAGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTCGGCGGCGATCATCTGGGGCCAAACTGCTGGCAGCAAGAAAATGCGGATGCGGCGATGGAAAAATCCGTCGAGCTGGTAAAGGAATATGTTCGTGC   >  1:266811/1‑251                                                                                                                                                                                                                                  AGCACGCGCAAAGTGCTGGCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTCGGCGGCGATCATCTGGGGCCAAACTGCTGGCAGCAAGAAAATGCGGATGCGGCGATGGAAAAATCCGTCGAGCTGGTAAAGGAATATGTTCGTGCC  >  1:76559/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              CCCGGGATTATTTGCAGTCGGCTAAATCCGCAATGCGCGATGTGGTGAGCAAAGTGATTGCCGATTGTGGCTGCGAGGGCAGGGCATAACGCACCTGCCATTTAACAAGGAAAAAACATGAAAACGTTAATTGCCCGGCATAAAGCTGGTGAACATATCGGCATATGTTCAGTCTGTTCTGCCCATCCGTTGGTTATCGAAGCGGCGCTGGCATTTGATCGCAACAGCACGCGCAAAGTGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2176438‑2176196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCAAAGTGCTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAATTTGTTTTTACGATTGCCGATAAAGTTGGGTTTGCACGCGAACGCATTATTCTCGGCGGCGATCATCTGGGGCCAAACTGCTGGCAGCAAGAAAATGCGGATGCGGCGATGGAAAAATCCGTCGAGCTGGTAAAGGAATATGTTCGTGCC  <  NC_000913/2176206‑2175974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.