Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,034,134	IS30 (–) +2 bp	coding (881‑882/1794 nt)	hyaB →	hydrogenase 1 large subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 271761	NA (NA)	25 (0.900)	24/486	0.3	96.1%	noncoding (1221/1221 nt)	IS30	repeat region
	?	NC_000913	= 1034135	1 (0.040)					coding (882/1794 nt)	hyaB	hydrogenase 1 large subunit
*	?	NC_000913	1034134 =	0 (0.000)	25 (0.910)	25/484	0.2	100%	coding (881/1794 nt)	hyaB	hydrogenase 1 large subunit
	?	NC_000913	1469296 =	NA (NA)					noncoding (1/1221 nt)	IS30	repeat region

TGTACCACGGGTCGGTGATGCCATCGAACGGATGACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1034376‑1034134 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACACTGTC  >  NC_000913/1469296‑1469542                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            TGTACCACGGGTCGGTGATGCCATCGAACGGATGACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGAT                                                                                                                                                                                                                                                  <  1:35393/250‑1              GGTGATGCCATCGAACGGATGACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACG                                                                                                                                                                                                                                    >  2:120991/1‑251                      CATCGAACGGATGACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGCATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTT                                                                                                                                                                                                                            >  2:924/1‑251                                                    TGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTT                                                                                                                                                                                              >  2:175874/1‑251                                                                              ACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGA                                                                                                                                                                    <  2:150531/251‑1                                                                                    TCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAGAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAA                                                                                                                                                               >  1:261151/1‑250                                                                                          ACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTC                                                                                                                                                        >  1:85548/1‑251                                                                                                    GATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTTCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAA                                                                                                                                              <  1:178532/251‑1                                                                                                            AAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAA                                                                                                                                      <  1:6681/251‑1                                                                                                                 CACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACG                                                                                                                                 >  1:103438/1‑251                                                                                                                 CACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACG                                                                                                                                 <  2:85548/251‑1                                                                                                                    TGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGA                                                                                                                               >  1:78352/1‑250                                                                                                                     GGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAAC                                                                                                                             <  1:226726/251‑1                                                                                                                       CAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGCGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAA                                                                                                                                                               >  2:57742/1‑215                                                                                                                       CAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAA                                                                                                                                                               <  1:57742/215‑1                                                                                                                              TTGTTGAAGTCTCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTC                                                                                                                      >  2:81257/1‑249                                                                                                                               TGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAG                                                                                                                    <  1:255437/250‑1                                                                                                                                           CGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAAT                                                                                                       >  1:42246/1‑251                                                                                                                                                    CCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGT                                                                                              >  2:4232/1‑251                                                                                                                                                                 CATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAA                                                                                 <  1:7969/251‑1                                                                                                                                                                       CAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGACTGCCACGAAGCTGAGAACGCATTTATCAGCAAGACCAGTGTAGATTCAATTGGTCAACGCCACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAAAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCT                                                                           >  1:35192/1‑251                                                                                                                                                                                           CGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATAC                                                        <  2:89327/250‑1                                                                                                                                                                                                   ATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCAT                                               <  2:51980/251‑1                                                                                                                                                                                                                                GCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGT                    >  1:69457/1‑249                                                                                                                                                                                                                                                CCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCAGGAGCGTAAGCGGGCTGTAGCTCACCTGACACTGTC  <  2:277966/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            TGTACCACGGGTCGGTGATGCCATCGAACGGATGACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1034376‑1034134 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACACTGTC  >  NC_000913/1469296‑1469542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.