Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,034,134	IS30 (–) +2 bp	coding (881‑882/1794 nt)	hyaB →	hydrogenase 1 large subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 271761	NA (NA)	25 (0.900)	24/486	0.3	96.1%	noncoding (1221/1221 nt)	IS30	repeat region
	?	NC_000913	= 1034135	1 (0.040)					coding (882/1794 nt)	hyaB	hydrogenase 1 large subunit
*	?	NC_000913	1034134 =	0 (0.000)	25 (0.910)	25/484	0.2	100%	coding (881/1794 nt)	hyaB	hydrogenase 1 large subunit
	?	NC_000913	1469296 =	NA (NA)					noncoding (1/1221 nt)	IS30	repeat region

AGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAa‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/271521‑271761 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCGACAATCCAGTTTGGATGCGGGTTTTTACCGCCAAAGACCGCGTGGATTTTGACAATTTCACGCTGGAA  <  NC_000913/1034135‑1033887                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            AGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATT                                                                                                                                                                                                                                                 <  1:263002/251‑1   CACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTC                                                                                                                                                                                                                                               <  2:274458/251‑1                                        ccgTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGC                                                                                                                                                                                                           <  2:180475/247‑1                                                  GGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATC                                                                                                                                                                                                 >  1:8673/1‑250                                                         AAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCCATATACTCAACATGAATTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTT                                                                                                                                                                                         >  2:108293/1‑251                                                                   ACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAG                                                                                                                                                                               <  2:40836/251‑1                                                                                      AGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATA                                                                                                                                                             >  2:247277/1‑250                                                                                           TTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGCTACTTGAC                                                                                                                                                       >  2:104331/1‑251                                                                                             TCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACT                                                                                                                                                      <  1:148966/250‑1                                                                                                        CATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCA                                                                                                                                          <  2:12905/251‑1                                                                                                                    AATATACTCAACATGAACTAGATCTGGTTGATGCCCCGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGCAAGAGATAATTGACAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATAT                                                                                                                              >  2:172271/1‑251                                                                                                                            CAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTG                                                                                                                        <  2:31087/249‑1                                                                                                                                                            AAACAACAGACCGAGAAAGACACTGAAGTTCAAAACATCGAAAGAGATAATTGAACGGGGAGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCACCACGCTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGGTCAGGCGTTACATATTGACTGCCCCGACCGCGCCGCATTCGTCAATGTTGATGGC                                                                                      >  2:40249/1‑251                                                                                                                                                            AAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATG                                                                                        <  2:216487/249‑1                                                                                                                                                                        GAGAAAGACCCTGAAGTTCAAAACACAGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCAGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCac                                                                          >  1:68116/1‑249                                                                                                                                                                             AGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCG                                                                      >  1:244667/1‑250                                                                                                                                                                                  CTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCGACAATC                                                                >  1:90987/1‑251                                                                                                                                                                                          CAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCTTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAAT                                                                                               <  2:102154/212‑1                                                                                                                                                                                          CAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAAT                                                                                               >  1:102154/1‑212                                                                                                                                                                                            AAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCGACAATCCAGTTTGGAT                                                      >  2:72656/1‑251                                                                                                                                                                                                      AGAGATAATTGAAAGGGGTGTTGCATTGACATATTGAATCTACAAGTGCCTATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCGACAATCCAGTTTGGATGCGGGTTTTT                                            <  1:45875/251‑1                                                                                                                                                                                                      AGAGATAATGGAAAGGGGTTTTGCATTGACAGATTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCGACAATCCAGTTTGGATGCGGGTTTgt                                            <  1:45877/251‑3                                                                                                                                                                                                                                       TTGAATCTACAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCGACAATCCAGTTTGGATGCGGGTTTTTACCGCCAAAGACCGCGTGGCTTTTGACAATTTC           >  2:259084/1‑251                                                                                                                                                                                                                                                CAAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGCTAATGAAGTCC‑CCGTGCGGGTGATAATTGACTGCACCAGGTTCCGGCGGTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCCTCCCGCCGACAATCCCGTTTGGATGCGGGTTTTTACCGCCAAAGACCGCGTGGATTTTGACAATTTCACGCTGGAA  >  2:150458/1‑250                                                                                                                                                                                                                                                 AAGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCGACAATCCAGTTTGGATGCGGGTTTTTACCGCCAAAGACCGCGTGGATTTTGACAATTTCACGCTGGAA  >  1:53138/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            AGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAa‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/271521‑271761 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGTGCCGATTTCGCTCCACGGTTTGTTGAACTGACCGATGGCTAAGGCGTCGGGGATCATCACGTTGTTAATGAAGTCCGCCGTGCGGGTGATAATTGACTGCACCAGGTTCAGGCGTTCCATATTGACTGCCCCGACCGCGCCGCTTTCGTCAATGTTGATGGCGCAAGGCATCCCGCCGACAATCCAGTTTGGATGCGGGTTTTTACCGCCAAAGACCGCGTGGATTTTGACAATTTCACGCTGGAA  <  NC_000913/1034135‑1033887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.