Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 305,664 IS2 (–) +5 bp coding (1150‑1154/1644 nt) ecpD ← fimbrial adhesin EcpD

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 305664 =0 (0.000)13 (0.850) 8/304 0.4 100% coding (1154/1644 nt) ecpD fimbrial adhesin EcpD
?NC_000913 381260 = NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 3056680 (0.000)18 (1.160) 15/308 0.1 100% coding (1150/1644 nt) ecpD fimbrial adhesin EcpD
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

GGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCG  >  NC_000913/381024‑381499
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
ggTAGCGATAATTCCCTCTGGAGTTCGAACATACACGTGCTGTTCATCCATGCGATGATGACTGGTACGTACTTGTGCTCCTAAATATGCCGGGTTTGCAACTACCGAGGCGGCAATAAAACAGTGCGGACCAATCGTTGCGTCCGCTTCAATAACCGCATGTGTAAGCTGGGTGTCAAAACCAATGTTTAGGCCGTTGCTTATTATTGGGTCAGGACGAATAAACGCATAATTACTAGACTGTCGCCCTg                                                                                                                                                                                                                                   <  1:227552/251‑1 (MQ=255)
                      gTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATCACCGCATTTTTAATCTTGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATc                                                                                                                                                                                                              >  1:347200/1‑250 (MQ=255)
                      gTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGAGCCTACATATGCCTGGTTTGCAACCACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTTGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATc                                                                                                                                                                                                              >  1:347196/1‑250 (MQ=255)
                          gAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGCGCGCATAAATATGCCTGGTTTGCAACTACCGAGTAGGCAATAAACCATTGCGGACCAATCGTTGCTTCCGCTTCCATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGCCGAATCAACGAATAATTACTAGACTGGCCCCCTGAATCTCCCAACAACCAATATCACtt                                                                                                                                                                                                          >  1:331012/1‑250 (MQ=255)
                                 cacGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGt                                                                                                                                                                                                  <  1:130486/251‑1 (MQ=255)
                                                               ngtacgtacTTGTGCGACTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGt                                                                                                                                                                    >  1:103171/2‑251 (MQ=255)
                                                                gtacgtacTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATACCCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGACTCTCCAGACAACCAATATAACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTc                                                                                                                                                                   >  1:241868/1‑251 (MQ=255)
                                                                   cgtacTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGCTTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTCGAc                                                                                                                                                                        >  1:309415/1‑243 (MQ=255)
                                                                                           ngantttncnntNNCGANTCGGCAATAAAACATTGCGGANNAATCGTTGCNTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCNTTGCTGATTNNTGNGCCAGNANNNANNNACNCATAATTACTAGACTGGCCNCCNNAATCTCNAGACAACCAATATCACTTAAATAAGt                                                                                                                                                                                                  <  2:83214/182‑1 (MQ=38)
                                                                                                  naaCTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCgg                                                                                                                                 >  1:70684/2‑251 (MQ=255)
                                                                                                    aCTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGgan                                                                                                                               <  1:209858/251‑2 (MQ=255)
                                                                                                         cGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGt                                                                                                                                                                                                  >  1:83214/1‑179 (MQ=255)
                                                                                                                                    aaTCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTTATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTTGCCCCCTGAATCTCCAGACAACCAATATCTCTTAAATAATTGATAGTCTTAATACTATGTTTTAGACTAGTCAGTGGATAACAGATGTTTTATGTCTTAGGGCCGGATAAACGCAGACGGCGTACCACACAGGAAAAGAt                                                                                               <  1:340471/251‑1 (MQ=25)
                                                                                                                                     aTCGTTGCTTCCGCTTCAAGAACCGCATTTTTAATCTCGGGGGCAAAACCAATTGTTACGCCATTGCGGATTATTGTGCCAGGACGAAAAAACGCATAATTACTAGACTGGCCCCCGGAATCGCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGGTTTGATAGTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGc                                                                                                                  <  1:48418/231‑1 (MQ=21)
                                                                                                                                           gCTTCCGCTTCAATAAACGCATTTTTCATCTCGGTGGCACAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAAtt                                                                                        >  1:297219/1‑251 (MQ=255)
                                                                                                                                             ttCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCGCCAGACAACCAATATCACTTAAATAAGTGATAGGCTTAATACTAGTTTTTATACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTg                                                                                       <  1:117679/250‑1 (MQ=255)
                                                                                                                                                 gCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTc                                                                                    >  1:147947/1‑249 (MQ=255)
                                                                                                                                                                       tctcGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCCTTGAACCGGGGATg                                                             >  1:121462/1‑250 (MQ=255)
                                                                                                                                                                        ctcGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTCGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGAAATTGTTCAGCAGAGCTTTGAACCGGGGATGAc                                                           >  1:226864/1‑251 (MQ=255)
                                                                                                                                                                               gCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGAACGAATAACCGCACAATTACTAGACTGGCCCCCTGAATCACCAGACAAACAATATCCCTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTAATGTCTTAGGGCCGGAGAAACGAAGa                                                                                                                      >  1:342634/1‑185 (MQ=18)
                                                                                                                                                                                                                              aaaCGCATAATTACTAGACTTGCCCCCTGAATCTCCAGACAACCAATAGCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTg     <  1:263087/251‑1 (MQ=21)
                                                                                                                                                                                                                              aaaCGCATAATTACTAGACTGTCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAAGACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATGGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTg     <  1:263094/251‑1 (MQ=21)
                                                                                                                                                                                                                                 cGCATAATTACTAGACTGGCCCCCTGAAGCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGGCATTAGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTTAACCGGGTATGACGGTCTCCCTCGTTGCCCGTCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCg  <  1:320646/251‑1 (MQ=255)
                                                                                                                                                                                                                                     tAATTACTATACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCGTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGc                                                                                                                         <  1:313628/128‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
GGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCG  >  NC_000913/381024‑381499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.