Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 568449 =0 (0.000)3 (0.370) 3/334 0.5 100% coding (135/333 nt) emrE multidrug/betaine/choline efflux transporter EmrE
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 5684530 (0.000)9 (1.100) 6/332 0.2 100% coding (139/333 nt) emrE multidrug/betaine/choline efflux transporter EmrE
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

GTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCA  >  NC_000913/1467739‑1468101
                                                                                                                                                                           |                                                                                                                                                                                               
gtggtgAGTCGTCGGTAGATCTGAATGGAGATACAACAATCAGCGGAGAATTCCCACTGGGGTTTGCCGGATTTAGTCGGGTACATGATTAAGCTTTGCTGGAAATTGGCAGTGGCGCTAAGCTAACGATTGAGGATATTGAGAGTGTTGAACATCATGTGACAAGATCCCGGGATTTGCCTCGATATTTCCATTCATCTGTCATCACTTAACCCATTACAAGCCCGCTGCCGGAGATATTCCCGTGgcga                                                                                                                  <  1:109405/251‑1 (MQ=255)
                                                                      ggTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTACCAATGCAGGATATTGAACGTTTTGAACATCATGGGAAAAGAACCCTGGATTTGCCCCTATATTTCTAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGCTGCCATTCGTTATAATGCTCGAACGCCTCTGCACGGTTCTTtgct                                            >  1:113031/1‑251 (MQ=21)
                                                                                                  cNGGAAATTGGAAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCACACTATGCGGATGCCATTCGTTATAATGCTCGAACGCATCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATg                 >  1:131561/1‑250 (MQ=255)
                                                                                                            ncAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGCTACTGATGt       >  1:101827/2‑250 (MQ=255)
                                                                                                                 ggCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGAGTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATAACAAGCCCGTTGCCGCAGATATTTCCGTGGCGAGCGATAACCCAGCGCACTATGCGGCTGACATTCTTTACAATGCTCGAACGCCTCTTCAAGGCTCTTTGCTGCCTTTAACCCGTCTGTTTTGGGCATGATACTGATGTAGTCa  <  1:154269/250‑1 (MQ=17)
                                                                                                                                                              gggACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTNNCGAGCGNNa                                                                                                            >  2:9516/1‑99 (MQ=255)
                                                                                                                                                              gggACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCgc                                       <  1:9516/168‑2 (MQ=38)
                                                                                                                                                                           |                                                                                                                                                                                               
GTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCA  >  NC_000913/1467739‑1468101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.