Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 568449 =0 (0.000)3 (0.370) 3/334 0.5 100% coding (135/333 nt) emrE multidrug/betaine/choline efflux transporter EmrE
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 5684530 (0.000)9 (1.100) 6/332 0.2 100% coding (139/333 nt) emrE multidrug/betaine/choline efflux transporter EmrE
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

GCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAG  >  NC_000913/1469025‑1469436
                                                                                                                                                                                                                       |                                                                                                                                                                                                    
gctACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTGCTCCGTCCCGAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTATTATTAAGACTATCACTTACTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGGCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCg                                                                                                                                                                    <  1:169440/250‑1 (MQ=255)
      ccATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGTCTAGTCTAAAAACTAGTATTAAGACTACCACTTAGTTAAGTGATATTGGTTGTCTGGAGATTCAGTGGGCCAGTCTAATCCCCAGAACATACTTGTGCTGATTCCGGTGCGAAAATTg                                                                                                                                                             <  1:134878/251‑1 (MQ=14)
                   cAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTAc                                                                                                                                                <  1:59259/251‑1 (MQ=255)
                                     tCCCCGGTTCAAAGCTATGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGa                                                                                                                                         >  1:51711/1‑240 (MQ=255)
                                                             cAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTACTCCGGCCATAAGACATCAATCATCTATTCTCCAATGAATAGTATAACAAATAGCATTAAAACTATCACTTATTTAAGTGATCTTGGTTGTCTGTAGATTAAGGGGGCAAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTTAATTGGTCAACGc                                                                                                                         >  1:27388/1‑232 (MQ=18)
                                                                               ntgtgGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCACACATCTGTTCTCCAATGCCTAGTCTAAAAACTAGTATTAAGACTATCAATTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGttttt                                                                                    >  1:30145/2‑251 (MQ=255)
                                                                                                        cGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagcag                                                           <  1:53650/251‑1 (MQ=255)
                                                                                                                                                                  aCTATCACTTATTTAAGTGAGATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCTGAACTTACTTATTCTGATTCCGGTGCTTAAATTGTTAATCAATGTTCTTGTCGGTTCATTTGGTCTACGCAGCATTTATATTAAATCATTTGGTTCCGGCGGTTTTTTGAATGGGCCGATCTGTTACAGGCGAGGAAAAAGCCTCTGTTTCTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAg  <  1:145570/250‑1 (MQ=16)
                                                                                                                                                                                                                       |                                                                                                                                                                                                    
GCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAG  >  NC_000913/1469025‑1469436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.