Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 568449 =0 (0.000)8 (0.800) 6/322 0.3 100% coding (135/333 nt) emrE multidrug/betaine/choline efflux transporter EmrE
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 5684530 (0.000)12 (1.210) 9/320 0.1 100% coding (139/333 nt) emrE multidrug/betaine/choline efflux transporter EmrE
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

ATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTC  >  NC_000913/1469094‑1469471
                                                                                                                                                  |                                                                                                                                                                                                                                       
aTCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCCATCATCTGTTCTCCAATGACTAGTCTCAAAACTAGTATTACGACTATCACTTCTTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGAACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGc                                                                                                                                 >  1:86281/1‑251 (MQ=255)
                                                tcaataaTCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTCTCGCTTATTTACGCGATATTGGTTGTCTGGAGATTCAGGGGGCCCGTCTAAACCCCAGACCTTACTTATGCTGATTCCGGAGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTc                                                                                                                                                                 >  1:98691/1‑171 (MQ=21)
                                                         tGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTCTCACTTATTTAAGAGATATTGGTTGTCTGGAGATTCAGGGGGCAAGTCTAAACCCCAGACCATACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTTGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAg                                                                                                                             >  1:219047/1‑198 (MQ=37)
                                                                                                         ttAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTgg                         <  1:210332/250‑1 (MQ=255)
                                                                                                                               agaTTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAAGTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGATCAGTTACAGCAGAGAAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAAGAGCGAATATCCTGGGTTCAAAACCCGGAACGATCttn  <  1:25381/251‑2 (MQ=255)
                                                                                                                                                  |                                                                                                                                                                                                                                       
ATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTC  >  NC_000913/1469094‑1469471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.