Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,737,137	IS2 (+) +5 bp	coding (37‑41/825 nt)	bax ←	putative glycoside hydrolase Bax

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3187426	NA (NA)	16 (1.610)	11/320	0.0	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	3737137 =	0 (0.000)					coding (41/825 nt)	bax	putative glycoside hydrolase Bax
*	?	NC_000913	= 3737141	0 (0.000)	10 (1.000)	8/322	0.1	100%	coding (37/825 nt)	bax	putative glycoside hydrolase Bax
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

ATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187203‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCG  >  NC_000913/3737137‑3737385                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           ATAGCAGAGAGCTTCGTGAAAACGAGAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGGTCGCCACGGGAATATCTGCGGCATCGGGCGTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATG                                                                                                                                                                                                                                <  1:220989/251‑1              TCGTGAAAACGATAAAGCGGGACTACATCAGTATCATGCCCAAGCCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAAGGGCATCCGCATAGTGCGCTGGGTTAGCGCTCGCCACGGTAATATCTTGGGCAGCGGGCTTGTAATGGGTTAAGTGTTAACAGAGGTCTGGAAATAGAGGTGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATC                                                                                                                                                                                                                    <  1:104851/250‑1                                                      CAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTAT                                                                                                                                                                           >  1:63555/1‑251                                                                                               nGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCA                                                                                                                                                                                                                                                    >  1:133212/2‑137                                                                                               CGTNCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCA                                                                                                                                                                                                                                                    <  2:133212/137‑1                                                                                                              ACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCA                                                                                                                      >  1:69378/1‑248                                                                                                                                                               tcgGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAACCATCGCCCCATATCGTCn                                                                                                                                                                                                                                    <  1:62833/86‑2                                                                                                                                                                  GCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGA                                                                                                                                                                                                                             >  1:192848/1‑93                                                                                                                                                                    GGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTC                                                                >  1:12204/1‑248                                                                                                                                                                       AGCGGGCTTGTAATGGGTTA‑GTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGG                                                         <  1:5862/251‑1                                                                                                                                                                                  AATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATn                                                <  1:53066/250‑2                                                                                                                                                                                                        GTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGC                         <  1:18232/251‑1                                                                                                                                                                                                                       GGCAAATCCAAGAATCATCGCCCCATNTCGTCGNNTGNGAGTCAAAATCATCAGGTCNCCNNNTNNNANTTAATNATNNCAACACCTTNTATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCNAGAGCACCCNNAGGAATAGTCCAAATCTGAAANTATNNCNNGNGTTNAC                                                                     >  2:47319/1‑192                                                                                                                                                                                                                        nCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTAGGATTTAATCATTCCAACACCTTATATTTTTCACACATTTGAGAGTTGAATCTCAAATCATATCAAACATAGCTGTCAAGAGCACC                                                                                                              >  1:19383/2‑150                                                                                                                                                                                                                               CAAGAATCATCGCCCCATCTCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCG  >  1:75223/1‑251                                                                                                                                                                                                                               CAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTGGATCATTCAAACACCTTATATTTTTCTCCAATTTGAGAGTTGAGTCTCAAATCATATCAAAAATAGCTGTCTAGAGCACTCCTAGGAATAGTCCAAATCTGAAAATATGTCACGTGATACCGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATAGCAAAATTCAGC               >  1:158214/1‑238                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           ATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187203‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTTTTTGCGCG  >  NC_000913/3737137‑3737385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.