Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,105,376	IS2 (–) +5 bp	intergenic (+83/‑34)	csgC → / → ymdA	curlin chaperone/uncharacterized protein YmdA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	381260 =	NA (NA)	15 (1.060)	12/308	0.1	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	1105376 =	0 (0.000)					intergenic (+83/‑38)	csgC/ymdA	curlin chaperone/uncharacterized protein YmdA
*	?	NC_000913	= 1105380	0 (0.000)	12 (0.850)	11/310	0.2	100%	intergenic (+87/‑34)	csgC/ymdA	curlin chaperone/uncharacterized protein YmdA
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

AACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGG  >  NC_000913/381051‑381497                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              aaCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGCACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTa                                                                                                                                                                                                       >  1:157272/1‑250 (MQ=255)                                             cTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGAGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAg                                                                                                                                                                        >  1:13578/1‑237 (MQ=255)                                                   ngcCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAAc                                                                                                                                                       >  1:65052/2‑248 (MQ=255)                                                              gCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAAc                                                                                                                                                                                                                   <  1:100446/177‑1 (MQ=255)                                                              gCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACGAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTg                                                                                                                                                                                               <  1:309802/197‑1 (MQ=255)                                                                       gCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCTCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTACTGATTATTGTGCCAGGACTAATAAACGCATAATTACTAGACTGGCCCCCTGAAACTCTAGACGACCAATATCATTTAAATAAGTGATAGTCCTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGcc                                                                                                                                 <  1:330559/250‑1 (MQ=37)                                                                                     ngCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACg                                                                                                                  >  1:29621/2‑251 (MQ=255)                                                                                            aaaCATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACTCCATTGCTGATTATTTTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAAGCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACTGCGTAcc                                                                                                           <  1:109716/251‑1 (MQ=255)                                                                                                           aTCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTAGCAAAACCAATTTTTACGCCATTGCTGATTAATGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAACCTCCAGAAAAACCATATCACTTAAATATGTGATAGTATTAATACTAg                                                                                                                                                                               >  1:327867/1‑168 (MQ=255)                                                                                                                                     aTTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCtt                                                                                                                                                                                       <  1:118229/134‑1 (MQ=255)                                                                                                                                                  gTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAg                                                                                                                                     >  1:12111/1‑171 (MQ=255)                                                                                                                                                    ggCAANACCAATTTTTACGCCATTGCTGATTATTGTGNCNGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACNACCAATATCANTTANNTAAGNGNTNGTCTTAATACTAGTTTTTAGACTAGTCATNGGAGAACAGATGATTGATGTCGTNNGGNNNGANNAACGc                                                                                                                       <  2:236088/183‑1 (MQ=21)                                                                                                                                                    ggCAAAACCCATTTTTACGCCATTGCTGATTGTTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGAc                                                                                                                                                                                                                      >  1:92456/1‑88 (MQ=255)                                                                                                                                                    ggCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCAAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGCGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGc                                                                                                                       >  1:236088/1‑183 (MQ=255)                                                                                                                                                                     cGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGACCCCCTGAATCTCCAGACAACCACTATCACTTAAATAAGAGATAGTCTTCATAATAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTATTAGGGCCGGCGAAACGCAGACGGCGTACCACACAGGAAAAGATCg                                                                                           >  1:224121/1‑194 (MQ=17)                                                                                                                                                                                   attGAGCCAGGACGAATAAACGCATAATTACTCGACTGGCCCCCTGAATATCAAGACCACCAATCTCACTTACATAAGTGATAGTCTTCATACTAGTTTTTAGAATAGTAATAGGAGAACAGATGATTGATGACTTAGGGCGGGAGAAACGAAGACAGCGTACCACACAGGAAAAGATAGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGcagcc                    >  1:165161/1‑251 (MQ=14)                                                                                                                                                                                                     aaCGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTCAATAAGTGATAGTCTTAATACTCGTTTTTAGACTAGTCCTTGGAGAACAGATGATTGATGTCTTAGGTCCGGAGAAACGCAGCCGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGAAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGAGTagcagcagatcggacgcgcacacgt  >  1:143524/1‑232 (MQ=11)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              AACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGG  >  NC_000913/381051‑381497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.