Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,666,074 IS1 (+) +9 bp coding (640‑648/1281 nt) csiE → stationary phase‑inducible protein CsiE

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 279163 =NA (NA)15 (1.480) 12/298 0.0 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 2666074 = 0 (0.000)coding (640/1281 nt) csiE stationary phase‑inducible protein CsiE
* ? NC_000913 = 291401NA (NA)10 (0.990) 9/296 0.1 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 2666082 0 (0.000)coding (648/1281 nt) csiE stationary phase‑inducible protein CsiE

TTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGTTGCGGATCGAGAAGAGGATGCAGAGGAATATCTGGAAGCAATCATGGAGGCTCGTGTTACCGTTGCTGGCATGGGGCTGGTGATGGAGGTGCAGGATTACTTCGATGGCGAGGCCGATAGACTGGC  >  NC_000913/291282‑291582
                                                                                                                       |                                                                                                                                                                                     
ttGCCGTTACGCACCACCCCGGCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACAATAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGTTGCGGATCGAGAAGAGGATGCAGAGGAATATCTGGAAGCAATCATGGAGGCTCGTGTTACCGTTGCTGGCATggg                                                     <  1:17571/250‑1 (MQ=255)
     gTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGTCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGGTGCGGATCGATAAGAGGATGCAGAGGAATATCTGGAAGCAATCATGGAGGCTCGTGTTACCGTTGCTGGCATGGTGCTGGt                                               <  1:186497/251‑1 (MQ=255)
              caccCCGGCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACGCCATCATACACTAAATCAGTAAGTTGGCAGCATCCCCGAATTTTCAGCCCGGATGTTTCACGTGGCTATGTCGAAGATCGGGCGGATAATGTGGCTGATCCAGAATAGGATGCAGAGGATGTTCTGGAATCAATCATGGATGCTTGTGTTACCGTTGCTGGCATGGGGCTGGTGATGGAGGt                                      <  1:236504/251‑1 (MQ=21)
                 cccGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGGACCTATGTCGAATATCTGTCCGATAAGGTTGCGGATCGAGAAGAGGATGCAGAGGAATATCTGGAAGc                                                                                         <  1:234033/197‑1 (MQ=255)
                                                aTAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGTTGCGGATCGAGAAGAGGATGCAGAGGAATATCTGGAAGCAATCATGGAGGCTCGTGTTACCGTTGCTGGCATGGGGCTGGTGATGGAGGTGCAGGATTACTTCGATGGCGAGGCCGATAGACTg    >  1:100810/1‑251 (MQ=255)
                                                      aCAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCTGCCCGGATGTTTCCCGTACTTATGCCGAATAGCTGGCCGATAAGGTTGCGGATCGAGAAGAGGATGCAGAGGAATATCGGGAAGCAATCATGGAGGCTCGTGTTACCGTGGCTGGCATGGGGCTGGTGATGGAGGTGCAGGATTACTTCGATGGCGAGGCCGATAGACTGGc  <  1:118758/247‑1 (MQ=38)
                                                                                                             gcagcaTCACCGAATTTTCAGCCCGGATGTTGCACGTACCTATGTCGAATATCTGGCCGATAAGGTTGCGGAGCGAGAAGAGGATGCAGAGGAATATCTGGAAGCAATCATGGATGCTCGTGTTACCGTTGCTGGCATGGGGCTGGTGATGGAGGTGCAGGAtn                              <  1:23566/164‑2 (MQ=255)
                                                                                                                       |                                                                                                                                                                                     
TTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGTTGCGGATCGAGAAGAGGATGCAGAGGAATATCTGGAAGCAATCATGGAGGCTCGTGTTACCGTTGCTGGCATGGGGCTGGTGATGGAGGTGCAGGATTACTTCGATGGCGAGGCCGATAGACTGGC  >  NC_000913/291282‑291582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.