Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 568449 =0 (0.000)5 (0.670) 5/316 0.2 100% coding (135/333 nt) emrE multidrug/betaine/choline efflux transporter EmrE
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 5684530 (0.000)11 (1.490) 11/314 0.0 100% coding (139/333 nt) emrE multidrug/betaine/choline efflux transporter EmrE
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

AAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGG  >  NC_000913/1467663‑1468148
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
aaaaaCGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGAGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGCTATTCGGGTACAGGATGAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTgg                                                                                                                                                                                                                                              >  1:176712/1‑250 (MQ=255)
                    gTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTgac                                                                                                                                                                                                                                       <  1:163438/237‑3 (MQ=255)
                                                                                                                                                         cGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCGATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCGGCCGCAGATATTCCCGTGgcgagc                                                                                                                                                               <  1:54369/176‑1 (MQ=255)
                                                                                                                                                                                       ggCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGt                                                      >  1:151709/1‑251 (MQ=255)
                                                                                                                                                                                                  tacgctaACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTCCCCCTATCTTTCCAGACATCTGTTATCCCTTCACCCATTACAAGCCCGCTGCAGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCttt                                           >  1:95688/1‑251 (MQ=255)
                                                                                                                                                                                                                          naGTTTTGAACATCATGGGACAAGAACCCTGGATTTGACCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCtccc                                                                                                                                                                                    >  1:17572/2‑87 (MQ=255)
                                                                                                                                                                                                                                 gAACATCATGGGAAAAGAACCCTGGATTTGCCACTATATTTCCAGACATCAGTAATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTACCGTGGCGAGCGATAACACAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGACTCTGCAAGGTTCTTTGCTGCCGTCAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGTAGCTGTCTGCTATTCCGt            >  1:129338/1‑251 (MQ=11)
                                                                                                                                                                                                                                           ggACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATCCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCgg  >  1:11517/1‑251 (MQ=21)
                                                                                                                                                                                                                                            gACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGACCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACACAGCGCACTATGCGGATGACATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCgg  >  1:123344/1‑250 (MQ=21)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
AAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGG  >  NC_000913/1467663‑1468148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.