Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,303,073	Δ12 bp	coding (175‑186/921 nt)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1303072	2 (0.050)	21 (0.540)	21/488	0.8	91.3%	coding (174/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
	?	NC_000913	1303085 =	2 (0.050)					coding (187/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

CTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGG  >  NC_000913/1302839‑1303311                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   ggcaggagtgtcctgctccacggtgtctgatttttatcgcattacagaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttatttctatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTAttt                                                                                                                                                                                                                                <  1:38194‑M2/5‑1 (MQ=255)                           ctgatttttatcgcattacagaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttactgatccgTATTTCAGCTACCTGAAACAACTGGCGCACg                                                                                                                                                                                                      >  2:173256‑M2/221‑251 (MQ=255)                                            acagaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCg                                                                                                                                                                                     <  1:170667‑M2/48‑1 (MQ=255)                                               gaaggcacaggccagaaggtagggcaatgttaaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGttt                                                                                                                                                                                  >  1:4225‑M2/201‑251 (MQ=255)                                                 gAAGGGAGGGCGATGATAAAATTTATTCTAAGCCGCTGTCTGGAAGCGAGTAAGACGCTATTTATTCTTATTACTATTTCGGTCTTTATGACGCGCCTCGCGCCGGGAAGCCCTTTTAACGGCGAACGTACTTTACCGCCAGAAGTGAAGGCCAACATCGAAGCGAAATATCATCTTAATGATCCGATAATGACACCGTATTTAAGCCACCTGAAACAACTGGAGCACGGTGATTTCGGGCCATCGTTTa                                                                                                                                                                                 >  2:279520/1‑250 (MQ=255)                                                                             taaaatttattctacgtcgctgtctggaagcgattccgacgctatttattcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACtt                                                                                                                                                     <  1:11270‑M2/80‑1 (MQ=255)                                                                                              cgctgtctggaagcgattccgacgctatttattcttattactatttcgttctatatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTcc                                                                                                                                   <  1:14923‑M2/98‑1 (MQ=255)                                                                                                                              tcttattactatttcgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTa                                                                                                                                                                                                                                   <  2:8802‑M2/2‑1 (MQ=255)                                                                                                                                     actatttcgttctttatgatgcgcctcgcgccgggaagcccttttatcggcgaatgtactttaccgccagaagcgatggccaatatcggagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATAAAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCTGCAGCATTTTTCCTTGc                                                                                            >  2:68762‑M2/115‑251 (MQ=255)                                                                                                                                            cgttctttatgatgcgcctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAAAAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCTCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAAt                                                                                      >  2:170532‑M2/108‑250 (MQ=255)                                                                                                                                                            cctcgcgccgggaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTg                                                                     <  1:213433‑M2/160‑1 (MQ=255)                                                                                                                                                                       gaagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGcc                                                          <  1:305067‑M2/171‑1 (MQ=255)                                                                                                                                                                        aagcccttttaccggcgaacgtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCg                                                         <  2:4225‑M2/172‑1 (MQ=255)                                                                                                                                                                                    cggcgaacgtactttaccgccagaactgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTGTAAATATAAATATCATTCGGTCAATGACTTGGTTGCATCCAGTTTGCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCAGTAATACTGGGTGTTAGTGCTGGCGGTATTGCCGCATTaaaacaaa                                             <  2:39913‑M2/184‑1 (MQ=255)                                                                                                                                                                                            gtactttaccgccagaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCaaa                                     <  1:40798‑M2/192‑1 (MQ=255)                                                                                                                                                                                                    aTGGCCAATATCGAAGCGAAATATCCTCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAACCAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACta                             <  1:279520/251‑1 (MQ=255)                                                                                                                                                                                                          gaagtgatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGt                       >  2:39227‑M2/46‑251 (MQ=255)                                                                                                                                                                                                               gatggccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATCCCGTGATgg                  >  2:210572‑M2/41‑251 (MQ=255)                                                                                                                                                                                                                    ccaatatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGAGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTg             >  1:332655‑M2/36‑251 (MQ=255)                                                                                                                                                                                                                       atatcgaagcgaaatatcatcttaatgatccgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGc           >  1:91646‑M2/33‑250 (MQ=255)                                                                                                                                                                                                                               gcgaaatatcatcttaatgatacgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTCGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCTGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCgg  >  2:216463‑M2/25‑251 (MQ=255)                                                                                                                                                                                                                                                     cgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATggg                                 >  2:27753‑M2/3‑198 (MQ=255)                                                                                                                                                                                                                                                     cgTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATggg                                 <  1:27753‑M2/196‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   CTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGG  >  NC_000913/1302839‑1303311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.