Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,303,073	Δ12 bp	coding (175‑186/921 nt)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1303072	2 (0.050)	21 (0.540)	21/488	0.8	91.3%	coding (174/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
	?	NC_000913	1303085 =	2 (0.050)					coding (187/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

GGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCT  >  NC_000913/1302827‑1303299                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    ggCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTTCTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtattt                                                                                                                                                                                                                                <  1:38194‑M1/251‑6 (MQ=255)                           cTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTACTGATCCGtatttcagctacctgaaacaactggcgcacg                                                                                                                                                                                                      >  2:173256‑M1/1‑220 (MQ=255)                                            aCAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcg                                                                                                                                                                                     <  1:170667‑M1/251‑49 (MQ=255)                                               gAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgttt                                                                                                                                                                                  >  1:4225‑M1/1‑200 (MQ=255)                                                             gAAGGGAGGGCGATGATAAAATTTATTCTAAGCCGCTGTCTGGAAGCGAGTAAGACGCTATTTATTCTTATTACTATTTCGGTCTTTATGACGCGCCTCGCGCCGGGAAGCCCTTTTAACGGCGAACGTACTTTACCGCCAGAAGTGAAGGCCAACATCGAAGCGAAATATCATCTTAATGATCCGATAATGACACCGTATTTAAGCCACCTGAAACAACTGGAGCACGGTGATTTCGGGCCATCGTTTa                                                                                                                                                                     >  2:279520/1‑250 (MQ=255)                                                                             tAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgactt                                                                                                                                                     <  1:11270‑M1/250‑81 (MQ=255)                                                                                              cGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTATATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcc                                                                                                                                   <  1:14923‑M1/251‑99 (MQ=255)                                                                                                                              tCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGta                                                                                                                                                                                                                                   <  2:8802‑M1/123‑3 (MQ=255)                                                                                                                                     aCTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTATCGGCGAATGTACTTTACCGCCAGAAGCGATGGCCAATATCGGAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaataaaaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagctgcagcatttttccttgc                                                                                            >  2:68762‑M1/1‑114 (MQ=255)                                                                                                                                            cGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaaaaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttctcgtttctgccaaactgggagccgcagcatttttccttgcggtaat                                                                                      >  2:170532‑M1/1‑107 (MQ=255)                                                                                                                                                            ccTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctg                                                                     <  1:213433‑M1/251‑161 (MQ=255)                                                                                                                                                                       gAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgcc                                                          <  1:305067‑M1/251‑172 (MQ=255)                                                                                                                                                                        aaGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccg                                                         <  2:4225‑M1/251‑173 (MQ=255)                                                                                                                                                                                    cGGCGAACGTACTTTACCGCCAGAACTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtgtaaatataaatatcattcggtcaatgacttggttgcatccagtttgcccgtttctgccaaactgggagccgcagcatttttccttgcagtaatactgggtgttagtgctggcggtattgccgcattaaaacaaa                                             <  2:39913‑M1/251‑185 (MQ=255)                                                                                                                                                                                            gTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaa                                     <  1:40798‑M1/251‑193 (MQ=255)                                                                                                                                                                                                          gAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgt                       >  2:39227‑M1/1‑45 (MQ=255)                                                                                                                                                                                                               gATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactatcccgtgatgg                  >  2:210572‑M1/1‑40 (MQ=255)                                                                                                                                                                                                                aTGGCCAATATCGAAGCGAAATATCCTCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAACCAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACta                 <  1:279520/251‑1 (MQ=255)                                                                                                                                                                                                                    ccAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggagcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgtgatggggctg             >  1:332655‑M1/1‑35 (MQ=255)                                                                                                                                                                                                                       atatCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatgggactataccgtgatggggctggc           >  1:91646‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                               gCGAAATATCATCTTAATGATACGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggtcgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgctgcattaaaacaaaacaccaaatgggactataccgtgatggggctggcaatgaccgg  >  2:216463‑M1/1‑24 (MQ=255)                                                                                                                                                                                                                                                     cGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatggg                                 >  2:27753‑M1/1‑2 (MQ=255)                                                                                                                                                                                                                                                     cGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacaccaaatggg                                 <  1:27753‑M1/198‑197 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    GGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCT  >  NC_000913/1302827‑1303299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.