Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,269,876	IS5 (+) +4 bp	intergenic (+309/‑100)	lpxT → / → mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2269876 =	0 (0.000)	20 (0.730)	20/488	0.4	100%	intergenic (+309/‑103)	lpxT/mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
	?	NC_000913	2288919 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 2269879	0 (0.000)	18 (0.650)	17/490	0.6	100%	intergenic (+312/‑100)	lpxT/mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

GAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATC  >  NC_000913/2269646‑2270124                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         gataaccaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACAt                                                                                                                                                                                                                                      <  1:154890‑M1/21‑1 (MQ=255)       caactggcgatgttattcccgctggccaccctgtttcgggcggaccaaatgataagtcagtgggggagatctcactaaaaactgagaataacgtcttaaatggcgaagaaacggtctaaataggctgattcaaggcatatacgggagaaaaaatcgactcacacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccgtccCTTAAGGAATTGTTTCAACATGccc                                                                                                                                                                                                                                  <  2:122644‑M1/25‑1 (MQ=255)                    tattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaacctggggctaacgccttaaatggcgaagaaacggtctaaacaggctgattcaaggcatttacgggagaaaaaatcggctaaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCg                                                                                                                                                                                                                   <  2:69271‑M1/40‑1 (MQ=255)                     attcacgctggccaacctgtttcgggctgaccaaatgatacggcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgatatgactgagtcagccgagaataatttccccgcgtattcgcaccgtccCTGAAGGAATTGTTTCAACATGACCAGGTAATTAGTCTCgt                                                                                                                                                                                                                  >  1:33445‑M1/211‑251 (MQ=255)                             tggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactacaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTg                                                                                                                                                                                                          <  2:135508‑M1/49‑1 (MQ=255)                                  gATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagc                                                                                                                                                                                                     <  1:248810‑M1/251‑51 (MQ=255)                                    cctgtttcgggcggacaaaatgatacgtcagtgggcgagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGGGTCGCTTGGCAtttt                                                                                                                                                                                                   <  1:241316‑M1/56‑1 (MQ=255)                                         tGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccataga                                                                                                                                                                                               >  1:107738‑M1/1‑194 (MQ=255)                                           cgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCGTAAGGAATTGTTTCAACATGCCCATATAATTATGCTCGTGTCGCTTGGCATTTTTTTATaa                                                                                                                                                                                            >  1:244466‑M1/189‑251 (MQ=255)                                             ggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACg                                                                                                                                                                                          >  1:34786‑M1/187‑251 (MQ=255)                                                                        aaCCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacctt                                                                                                                                                               >  1:46466‑M1/1‑163 (MQ=255)                                                                               cactaaaaactggggacaacgcgttaaatggcgaagaaactgtctaaataggctgtatcaaggcatttacgggagaaaacatcggctcaaacatggaaaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgtaccttccCGTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTAGTGTCGCGTGGAATTTTTTTATAGCGATATTTCTCTTTAAGGACTTCAAGGGAaagcaaa                                                                                                                                                        >  2:210862‑M1/153‑251 (MQ=255)                                                                                                     ttttCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgcc                                                                                                                                  >  2:45821‑M1/1‑134 (MQ=255)                                                                                                            ggcgaagaagcggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGGCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTga                                                                                                                           >  1:37672‑M1/124‑251 (MQ=255)                                                                                                                  aaTCATGTAAACCCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGACGATTATCCTCCCAGCATATAATTTTGCGCGTTAGTCCCCAGATTCGGCCTTAggaaggtgcgaaccagtccctgatctgagatcatgtttgtcatttggggccatagaacagggttcatcatgagccatcaacttaccttcgccgacagtgaattcagcagtaagcgccgacagaccagaa                                                                                                                      >  2:36762‑M1/1‑121 (MQ=255)                                                                                                                      cggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCtt                                                                                                                 <  1:30954‑M1/138‑1 (MQ=255)                                                                                                                       ggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgcagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTg                                                                                                                <  1:42689‑M1/139‑1 (MQ=255)                                                                                                                           aacacgcccgccaagacCGCACCAATCTGGTAATCTCGACCTCGTTTTGCGTCGGCGGTAGATTATCCTCACAGCATATAATGGTGTGCGTTAGTCCACAGATTTTGCCTTAtgaaggtgcgaacaaggccctgatatgagatcatgtttgtcatctggagccatagaacagtgttcatgatgagtcatcaacctacctactccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattct                                                                                                            <  2:46463‑M1/243‑140 (MQ=255)                                                                                                                           aaaCCGCGCGCCAAGACCGCACCAATCGGGTAATCTCGACCTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATGTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccagagaacagggttcatcatgagtcatcaacttaccttcgccgacattgaattcagcagtaagcgccgtcagaccagaaaagagagttt                                                                                                            <  2:34404‑M1/251‑140 (MQ=255)                                                                                                                                     ggttcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaagttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCcg                                                                                                   >  2:264232‑M1/99‑250 (MQ=255)                                                                                                                                           aggcatttacgggagaaaaaagcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTTGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGc                                                                                            >  1:21502‑M1/93‑251 (MQ=255)                                                                                                                                             gCACCAATCGGTTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTTTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagctcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcattgngc                                                                                           >  2:260170‑M1/1‑94 (MQ=255)                                                                                                                                               atttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgaatgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCCGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGt                                                                                         <  1:136877‑M1/162‑1 (MQ=255)                                                                                                                                                tttacgggagaacaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttcaccgcttattcgaaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGt                                                                                         <  2:160958‑M1/162‑1 (MQ=255)                                                                                                                                                      gggTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgcc                                                                                 >  1:104469‑M1/1‑85 (MQ=255)                                                                                                                                                       gagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCt                                                                                <  1:62203‑M1/171‑1 (MQ=255)                                                                                                                                                                                   ggTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagc                                                    <  2:87722‑M1/251‑196 (MQ=255)                                                                                                                                                                                    gTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagcc                                                   >  1:160584‑M1/1‑55 (MQ=255)                                                                                                                                                                                            tCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttacc                                           >  1:232860‑M1/1‑47 (MQ=255)                                                                                                                                                                                                       gagaagaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCATCCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTga                                >  1:135508‑M1/33‑251 (MQ=255)                                                                                                                                                                                                            gaatttccccgcttattcgcaccttccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGAGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATTTAGTGCAAATAACAACGCAAAGAATATGCATcctgagaacc                           <  2:199850‑M1/224‑4 (MQ=255)                                                                                                                                                                                                                    gTTAGTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgac                   >  2:186630‑M1/1‑23 (MQ=255)                                                                                                                                                                                                                        gTCCACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgacctta               <  1:45821‑M1/251‑233 (MQ=255)                                                                                                                                                                                                                          ccACAGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttat              <  2:232860‑M1/250‑234 (MQ=255)                                                                                                                                                                                                                             caGATTTGGCCTTAggaaggtgcgaacaagtccctgatatgagatcatgattgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagacgttttactccaaggctggtaatggccggctaccttatccgc          >  2:136809‑M1/1‑14 (MQ=255)                                                                                                                                                                                                                                    ggCCTTAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagtgattttcgtgtcccgcatggagcagattcagccatgg                                                                             <  1:76346‑M1/177‑171 (MQ=255)                                                                                                                                                                                                                                    ggCCTTAggaaggagcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtaagaccagaaaagagattttcttgtcccgcatggagcagattctgccatgg                                                                             >  2:76346‑M1/1‑7 (MQ=255)                                                                                                                                                                                                                                     ccCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACAACGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACatcatc  <  1:198523‑M1/249‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         GAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTGTGCGTTAGTCCACAGATTTGGCCTTAAGGAATTGTTTCAACATGCCCAGGTAATTAGTCTCGTGTCGCTTGGCATTTTTTTATAACGATATTTGTCGTTAAGGACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATC  >  NC_000913/2269646‑2270124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.