Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	257,908	Δ776 bp		insB9–[crl]	insB9, insA9, [crl]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	257908	258683	776	62 [0]	[2] 63	insB9–[crl]	insB9,insA9,[crl]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 257907	0 (0.000)	61 (0.990)	56/470	0.1	100%	intergenic (+8/+16)	crl/insB9	RNA polymerase holoenzyme assembly factor Crl/IS1 family transposase B
	?	NC_000913	258684 =	0 (0.000)					pseudogene (9/331 nt)	crl	RNA polymerase holoenzyme assembly factor Crl

CCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTC  >  NC_000913/257659‑258147                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 ccTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAa                                                                                                                                                                                                                                                 <  1:148316‑M1/250‑2 (MQ=255)       catcTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTGAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAATAGCAGATTGATCATAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaa                                                                                                                                                                                                                                           <  1:516689‑M1/250‑8 (MQ=255)       catcTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaa                                                                                                                                                                                                                                           >  1:268663‑M1/1‑243 (MQ=255)            gctgACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGAGCAAAAAAAGTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaag                                                                                                                                                                                                                                         <  1:526910‑M1/247‑10 (MQ=255)               gacgggatattattAGAGATCCCATTTAACCCGGTGTATAGGAATTTTGACAAAGGGCTTCAGGCAATAACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCGAATTTGGTAAAACCGTTGCATCACAACAGGAGAGAGGAACGACGTTACCGAGTGGACACCCGACGGGCGGATTGATCGATACACTTACCGCACTAGGCCCGTATTATT‑GTGAaggtaagggaaaagataatcg                                                                                                                                                                                                                                  >  2:6915/9‑235 (MQ=255)               gACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcg                                                                                                                                                                                                                                  >  1:169872‑M1/1‑235 (MQ=255)                  ggTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgatt                                                                                                                                                                                                                               <  1:427184‑M1/251‑20 (MQ=255)                                     cATTTAACCCGGTGGATCGGAATTTTGACCAAGGCCTTCAGGAAATCACCGACTGGATCGAACAACGCTTGTGTTAAAAATTTACTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGAAagtgaaaagataatcgattctttttcgattgtctgtct                                                                                                                                                                                                            >  2:241971‑M1/1‑213 (MQ=255)                                      aTTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctg                                                                                                                                                                                                           >  2:31572‑M1/1‑212 (MQ=255)                                      aTTTAACCAGGTGTATCGGAATTTTGAAAAAGGTCTTCAGGCTATCACCGACTGGATAGAACAACGATTGTGTTAAAAATTTGCTAAATTTTGAAAATTTGGCAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATAAAAAAATTTAACGCACTAGGCCGGCAT‑ATTCGTGAAGGTAagggcaaagataatagattcttgttcgtttgtctggctg                                                                                                                                                                                                           >  1:502042‑M1/1‑212 (MQ=255)                                         tAACCCGGTGTATCGGAATTCTGACAAAGGACTTCACGAAATCACCGACCGGACCGCAAAACGCTTGTGTTACACATTTGCTCAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgcttctttttcgattgtctggctgtat                                                                                                                                                                                                        >  2:526669‑M1/1‑209 (MQ=255)                                          aaCCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatg                                                                                                                                                                                                       <  2:571649‑M1/251‑44 (MQ=255)                                          aaCCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataaacgattctttttcgattgtctggctgtatg                                                                                                                                                                                                       <  2:268663‑M1/251‑44 (MQ=255)                                                        gAATTTTGAAAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATGTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGCGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATGTACCGCACTAGGACCCTAT‑ATTCGTGAAGGTAagtgcaaatataatcgagtctttgacgattgtctggctggatgcgtcaatgtgaaac                                                                                                                                                                                         <  2:279554‑M1/251‑58 (MQ=255)                                                         aaTTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATGTGCTAAATTTTGCCAAGTTGGTAAAACAGTTGCATCACAACAGTAGATAGCAATGACGTTACCGAGTGGTCACCCGAAGAGCAGATTGATCAAAAAATTTACCGCTCTAGGCCCGTAG‑AGGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                                                                                                                        <  1:288423‑M1/251‑59 (MQ=255)                                                              tGACAAAGGTCTTCAGGCAATCACCGACTGGCTAGAAAAACGCTTGTGTTAAACATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcac                                                                                                                                                                                   >  2:335931‑M1/1‑188 (MQ=255)                                                                               aaaTCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattc                                                                                                                                                                  <  1:20607‑M1/251‑81 (MQ=255)                                                                                     ccGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggc                                                                                                                                                            <  1:405330‑M1/251‑87 (MQ=255)                                                                                        aCTGGATAGAAAAAAGCTTGTGTTAAAAATTGGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCAAAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGTTCAAAAATTTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAggtgaaaagataatcgtttctttttcgatggtctggctgtatgcgtcaacgtgaaaccggaaccggaagtgcgtgaattctggggctgg                                                                                                                                                         >  1:452846‑M1/1‑162 (MQ=255)                                                                                                   aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAACACAGTTGCATCACAAAAGGAGATAGCACTGACGTTCCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagct                                                                                                                                              >  1:538513‑M1/1‑151 (MQ=255)                                                                                                       gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgga                                                                                                                                           >  1:510010‑M1/1‑147 (MQ=255)                                                                                                                 aaaaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACACCAGGAGATAGCAATGAAGTTACCGCGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaat                                                                                                                                >  1:500031‑M1/1‑137 (MQ=255)                                                                                                                   aaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGACTCAAAAAAGGCGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAACATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggcgctggaagcgcaggaatcc                                                                                                                              >  1:51733‑M1/1‑135 (MQ=255)                                                                                                                       tGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTCCCGCGTGGACACACGAAGAGCAGCTTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtt                                                                                                                          >  1:546949‑M1/1‑131 (MQ=255)                                                                                                                        gCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttt                                                                                                                         >  1:225680‑M1/1‑130 (MQ=255)                                                                                                                           aaaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCATTGACGGTACCGAGTGGACACCAGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGAAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                                                                                                                      >  1:71242‑M1/1‑127 (MQ=255)                                                                                                                             aTTTTGCAAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacct                                                                                                                    >  1:444439‑M1/1‑125 (MQ=255)                                                                                                                                  gCCAATTTGGTAAAACAGTTGCATCGCAACAGGAGATAGCAAGGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattcttgttcgattgtctggctgtatgcgtcagcgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag                                                                                                                <  2:4932‑M1/250‑131 (MQ=255)                                                                                                                                     aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaagtctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac                                                                                                            <  2:416953‑M1/251‑135 (MQ=255)                                                                                                                                        ttGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataagcgatgcttgttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgtaagtgcgtgaattctgtggcttgtggatggagcgggaagcgcaggaatcccgttttacctacagttacca                                                                                                          <  1:525672‑M1/250‑137 (MQ=255)                                                                                                                                           gTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTAAAGGAAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagagcgtgaattctggggctggtggatggagctggaagcgcaggcatcccgttttacctacagttaccagttt                                                                                                      >  1:419336‑M1/1‑111 (MQ=255)                                                                                                                                                aCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtct                                                                                                 <  1:124513‑M1/251‑146 (MQ=255)                                                                                                                                                    ttGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgtaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttaccta                                                                                                                   <  1:53524‑M1/229‑128 (MQ=255)                                                                                                                                                    ttGCACCACAACAGGAGATAGCAATGACGTTACCGAGTGGCCACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttaccta                                                                                                                   >  2:53524‑M1/1‑102 (MQ=255)                                                                                                                                                              acaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTTGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagc                                                                                     >  1:324964‑M1/1‑92 (MQ=255)                                                                                                                                                              acaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagc                                                                                     >  2:407218‑M1/1‑92 (MQ=255)                                                                                                                                                                aGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaagtctggggcttgtggatggagctggaagcgcaggaatcccgttttgcctacagttaccagtttggtctgttcgataaagcaggc                                                                                 <  1:99102‑M1/251‑162 (MQ=255)                                                                                                                                                                       aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactgga                                                                          <  2:192523‑M1/251‑169 (MQ=255)                                                                                                                                                                             gACGTTACCGAGTGGACCCCCGAAGAGCCGATTGATCACAAAATTTACAGCACTAGGCCCGTAT‑ATTCGAGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggacgtgcgtgaattctggggctggtggatggagctggaagcgcaggactcccgttttacctacagttaccagtttggtctgttcgatcaagcaggcgactggaagagtg                                                                    >  2:262480‑M1/1‑77 (MQ=255)                                                                                                                                                                                gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttc                                                                 >  1:60597‑M1/1‑74 (MQ=255)                                                                                                                                                                                   aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccg                                                               <  1:436710‑M1/250‑180 (MQ=255)                                                                                                                                                                                    ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggt                                                             <  1:15081‑M1/251‑182 (MQ=255)                                                                                                                                                                                              acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagggcgtgaattctggggctggtggatggagctggaagcgcaggaatcgcgtttaacctacaggtaccagtgtgggctgttcgagaaagcaggcggctggaagaatgttcctgtaaaagacact                                                   <  2:285571‑M1/251‑192 (MQ=255)                                                                                                                                                                                               cccGAAGAGCAGATTGATCAAAAAATTTACCGCACTTGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactg                                                  <  2:324964‑M1/251‑193 (MQ=255)                                                                                                                                                                                                        cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttg                                         >  2:11734‑M1/1‑50 (MQ=255)                                                                                                                                                                                                          gATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttga                                        >  2:188524‑M1/1‑48 (MQ=255)                                                                                                                                                                                                           aTTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaac                                      <  2:169872‑M1/251‑205 (MQ=255)                                                                                                                                                                                                           aTTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaac                                      <  1:309656‑M1/251‑205 (MQ=255)                                                                                                                                                                                                               aTCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgact                                  <  2:112078‑M1/251‑209 (MQ=255)                                                                                                                                                                                                                 cAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTCactgcaaagataatcgattctttttagattgtctggctgtatgcgtcaccgtgaaacaggcaccggaagtgcgtgaattctggggctggtggctggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggcagagtgttccggtcaaagacactgaagtggttgaacgactgg                                >  1:533874‑M1/1‑41 (MQ=255)                                                                                                                                                                                                                    aaaaTTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttgggtctgttcgataaagcaggcgactggaagagtgttgcggtaaaagacactgaagtggttgaacgactggagc                             <  1:262480‑M1/251‑214 (MQ=255)                                                                                                                                                                                                                           aCCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccct                      >  1:198630‑M1/1‑31 (MQ=255)                                                                                                                                                                                                                            ccGCACTAGGCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctg                     >  2:178188‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                             cGCACTAGGTCCGTAG‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattggctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaagtctggggctggtggatggagcgggaagcgcaggaatcccgttttacctacagttaccagtttggcctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaa                                                <  2:107945‑M1/223‑195 (MQ=255)                                                                                                                                                                                                                                 cTAGGCCCGGCT‑AGTCGTGAAGGGAagggcaaagataatcgattctttttcgattgtctggctgaatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgctaaagcaggcgactggaagagtgttccgggaaaagacactgaagtggttgaacgactggtgaccaccctgct                   >  2:56905‑M1/1‑25 (MQ=255)                                                                                                                                                                                                                                    ggCCCGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtt             >  1:420874‑M1/1‑22 (MQ=255)                                                                                                                                                                                                                                    ggCCCGTAT‑ATTCGTGAACGTAagtgcacagctactcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttgccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtt             >  2:357498‑M1/1‑22 (MQ=255)                                                                                                                                                                                                                                       ccGTAT‑ATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtttca          <  1:488686‑M1/251‑233 (MQ=255)                                                                                                                                                                                                                                           at‑atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtttcacg        >  1:9189‑M1/1‑15 (MQ=255)                                                                                                                                                                                                                                            t‑atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagac                                                      >  2:321632‑M1/1‑14 (MQ=255)                                                                                                                                                                                                                                            t‑atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagac                                                      <  1:321632‑M1/202‑189 (MQ=255)                                                                                                                                                                                                                                                tCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagcacaccctgcgtgagtttcacgagaagc  >  2:264819‑M1/1‑11 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 CCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAT‑ATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTC  >  NC_000913/257659‑258147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑