Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,077,568	Δ1,832 bp		[cmtA]–cmtB	[cmtA], cmtB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3077568	3079399	1832	37 [0]	[1] 38	[cmtA]–cmtB	[cmtA],cmtB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3077567	0 (0.000)	37 (0.420)	33/490	1.1	100%	coding (1293/1389 nt)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
	?	NC_000913	3079400 =	0 (0.000)					intergenic (‑70/+244)	cmtB/tktA	mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1

TTGTTAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAC  >  NC_000913/3079173‑3079647                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        cagacgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGgt                                                                                                                                                                                                                                  <  1:370232‑M2/24‑1 (MQ=255)         tgcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaattcgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggaggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatagcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGa                                                                                                                                                                                                                           <  2:250476‑M2/31‑1 (MQ=255)           cagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatggtcggcggttaattgagtaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAAGTGAGCAGGGAAACCGGTGTTTTGaaa                                                                                                                                                                                                                         <  2:650018‑M2/33‑1 (MQ=255)            agaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAAc                                                                                                                                                                                                                       >  1:241424‑M2/217‑251 (MQ=255)                attacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTg                                                                                                                                                                                                                   <  1:332351‑M2/39‑1 (MQ=255)                attacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtggcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTg                                                                                                                                                                                                                   <  1:579719‑M2/39‑1 (MQ=255)                     actcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAatt                                                                                                                                                                                                               >  2:80594‑M2/208‑250 (MQ=255)                        caccaggaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaaatttggttttcatttttacctctgtaatcagtgtttatgttcggcggataattgattaactaaagtgtcgaggtttggaacgcccatatagttattaatcaatatcagtggtttattcgtcaaaAAGCACTTGAGCAGGGAAACCGGTGATTTGAAAACAGTGAatgatt                                                                                                                                                                                                            >  2:56962‑M2/205‑250 (MQ=255)                          ccaggaattcattatctgtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatatcagctactttggttttcatttttacctctgtaatcagtgtttatgtgcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcattggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAAt                                                                                                                                                                                                         <  2:442139‑M2/49‑1 (MQ=255)                               aattcattatcagtaattcctgacagttcaaatccgcgcagacggacatccctcttgccataaatatcagcaactttggttttcatttttacctctgtaatcattgtttatgttcagcagttaattgattaaataaagggtcgagttttggatcgccaatatagttattaatcaatatcagtggtttaaccgtcacaAAGCAAATGAGCTGGGAAACCGGTGGTTTGAAAACAGTGAATTATTAAATTCtt                                                                                                                                                                                                    <  2:227819‑M2/54‑1 (MQ=255)                                   cattatcggtaatttcttgcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTtata                                                                                                                                                                                                 <  2:311858‑M2/57‑1 (MQ=255)                                     ttatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTATtatata                                                                                                                                                                                               >  1:264140‑M2/192‑250 (MQ=255)                                      tatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccaaaaatagcagcaactttggtgttcatttttacctctgtaatcagtgtgtatgttctgcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAAAGGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATaa                                                                                                                                                                                              <  1:415292‑M2/60‑1 (MQ=255)                                                 tctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTtat                                                                                                                                                                                   <  2:627872‑M2/71‑1 (MQ=255)                                                  ctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTtat                                                                                                                                                                                   >  1:433590‑M2/179‑249 (MQ=255)                                                        gttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGAtata                                                                                                                                                                            >  1:591935‑M2/173‑250 (MQ=255)                                                                acgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcattttttcctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACaaa                                                                                                                                                                    <  1:161650‑M2/86‑1 (MQ=255)                                                                acgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtggcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAAt                                                                                                                                                                   <  2:74890‑M2/87‑1 (MQ=255)                                                                    gcagaaggacatcccgcttgccataaatagcagcaacattggttttcatttttaccgctgtaatcagtgtgtatgttcggcggttaattgattaaataaagggtcgagttttggatcgccaatatagtgattaatcaatatcagtggtttatccgtcacaATGCTATTGAGCAGGTAAACCAGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACATCAAATGTCa                                                                                                                                                               <  2:56043‑M2/91‑1 (MQ=255)                                                                          ggacatcccgcttgccataaatagcagcacctttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCACTTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGc                                                                                                                                                         >  2:679014‑M2/155‑251 (MQ=255)                                                                                      tgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATg                                                                                                                                              <  1:150359‑M2/108‑1 (MQ=255)                                                                                            aaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATAtt                                                                                                                                        >  1:645635‑M2/137‑250 (MQ=255)                                                                                                agcagcaactttgggtttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttgtatcgctaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTTAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATTCATATTAcac                                                                                                                                    <  2:282108‑M2/118‑2 (MQ=255)                                                                                                          ttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGAtatt                                                                                                                          <  2:94975‑M2/128‑1 (MQ=255)                                                                                                                             ctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATAttt                                                                                                        <  1:388310‑M2/146‑1 (MQ=255)                                                                                                                               gtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTtg                                                                                                       <  2:128355‑M2/147‑1 (MQ=255)                                                                                                                                              gttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAat                                                                                                                                                                                                                <  2:214028‑M2/42‑1 (MQ=255)                                                                                                                                              gttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAat                                                                                                                                                                                                                >  1:214028‑M2/87‑128 (MQ=255)                                                                                                                                                 cggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATc                                                                                  <  1:357597‑M2/168‑1 (MQ=255)                                                                                                                                                        taattgatcaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTTATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGc                                                                            <  1:56962‑M2/174‑1 (MQ=255)                                                                                                                                                                   ataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAAc                                                                <  1:408555‑M2/186‑1 (MQ=255)                                                                                                                                                                                      atcgccaatatagttattaatcaatatcagtngtttatccgtcacaATGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGtaataa                                             <  2:370046‑M2/205‑1 (MQ=255)                                                                                                                                                                                         gccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATaaaa                                           >  2:645166‑M2/44‑250 (MQ=255)                                                                                                                                                                                                               atcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATTACAACAAATTTCAATACGCATATCGTTATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTAATCTATTGCTTGTCCGCTAACGGACAGATCAAGGTAATAAAAAAGGTCGACGAAGCGCccttgg                     <  2:403675‑M2/229‑3 (MQ=255)                                                                                                                                                                                                                              gtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt     <  1:80594‑M2/245‑1 (MQ=255)                                                                                                                                                                                                                              gtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt     <  1:446254‑M2/245‑1 (MQ=255)                                                                                                                                                                                                                                 acaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc  >  1:32356‑M2/4‑251 (MQ=255)                                                                                                                                                                                                                                   aaaGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc  >  1:48518/1‑249 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        TTGTTAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAC  >  NC_000913/3079173‑3079647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑