Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,077,568	Δ1,832 bp		[cmtA]–cmtB	[cmtA], cmtB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3077568	3079399	1832	37 [0]	[1] 38	[cmtA]–cmtB	[cmtA],cmtB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3077567	0 (0.000)	37 (0.420)	33/490	1.1	100%	coding (1293/1389 nt)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
	?	NC_000913	3079400 =	0 (0.000)					intergenic (‑70/+244)	cmtB/tktA	mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1

CAGACGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACACGTTTCACGCGCCCTTCCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACAAAGGCGATACGCTTAACCCTGCTTAACGAGAATGCACCCTCTTGTTTCATCGCCTTAACCGCATTGGCTGACTGAGCAAA  >  NC_000913/3077341‑3077815                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                         cAGACGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggt                                                                                                                                                                                                                                  <  1:370232‑M1/251‑25 (MQ=255)         tgcagAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATTCGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGAGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATAGCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttga                                                                                                                                                                                                                           <  2:250476‑M1/248‑32 (MQ=255)           cAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGGTCGGCGGTTAATTGAGTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaagtgagcagggaaaccggtgttttgaaa                                                                                                                                                                                                                         <  2:650018‑M1/250‑34 (MQ=255)            aGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaac                                                                                                                                                                                                                       >  1:241424‑M1/1‑216 (MQ=255)                aTTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtg                                                                                                                                                                                                                   <  1:332351‑M1/251‑40 (MQ=255)                aTTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGGCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtg                                                                                                                                                                                                                   <  1:579719‑M1/251‑40 (MQ=255)                     acTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaatt                                                                                                                                                                                                               >  2:80594‑M1/1‑207 (MQ=255)                        cACCAGGAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAAATTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGATAATTGATTAACTAAAGTGTCGAGGTTTGGAACGCCCATATAGTTATTAATCAATATCAGTGGTTTATTCGTCAAAaagcacttgagcagggaaaccggtgatttgaaaacagtgaatgatt                                                                                                                                                                                                            >  2:56962‑M1/1‑204 (MQ=255)                          ccAGGAATTCATTATCTGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATATCAGCTACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTGCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCATTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaat                                                                                                                                                                                                         <  2:442139‑M1/251‑50 (MQ=255)                               aaTTCATTATCAGTAATTCCTGACAGTTCAAATCCGCGCAGACGGACATCCCTCTTGCCATAAATATCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCATTGTTTATGTTCAGCAGTTAATTGATTAAATAAAGGGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTAACCGTCACAaagcaaatgagctgggaaaccggtggtttgaaaacagtgaattattaaattctt                                                                                                                                                                                                    <  2:227819‑M1/251‑55 (MQ=255)                                   cATTATCGGTAATTTCTTGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttata                                                                                                                                                                                                 <  2:311858‑M1/250‑58 (MQ=255)                                     ttATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttattatata                                                                                                                                                                                               >  1:264140‑M1/1‑191 (MQ=255)                                      tATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCAAAAATAGCAGCAACTTTGGTGTTCATTTTTACCTCTGTAATCAGTGTGTATGTTCTGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaaaggagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataa                                                                                                                                                                                              <  1:415292‑M1/250‑61 (MQ=255)                                                 tCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttat                                                                                                                                                                                   <  2:627872‑M1/250‑72 (MQ=255)                                                  cTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttat                                                                                                                                                                                   >  1:433590‑M1/1‑178 (MQ=255)                                                        gTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatata                                                                                                                                                                            >  1:591935‑M1/1‑172 (MQ=255)                                                                aCGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTTCCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaa                                                                                                                                                                    <  1:161650‑M1/250‑87 (MQ=255)                                                                aCGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGGCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaat                                                                                                                                                                   <  2:74890‑M1/251‑88 (MQ=255)                                                                    gcAGAAGGACATCCCGCTTGCCATAAATAGCAGCAACATTGGTTTTCATTTTTACCGCTGTAATCAGTGTGTATGTTCGGCGGTTAATTGATTAAATAAAGGGTCGAGTTTTGGATCGCCAATATAGTGATTAATCAATATCAGTGGTTTATCCGTCACAatgctattgagcaggtaaaccagtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacatcaaatgtca                                                                                                                                                               <  2:56043‑M1/251‑92 (MQ=255)                                                                          ggACATCCCGCTTGCCATAAATAGCAGCACCTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcacttgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgc                                                                                                                                                         >  2:679014‑M1/1‑154 (MQ=255)                                                                                      tGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatg                                                                                                                                              <  1:150359‑M1/250‑109 (MQ=255)                                                                                            aaaTAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatatt                                                                                                                                        >  1:645635‑M1/1‑136 (MQ=255)                                                                                                agcagcAACTTTGGGTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGTATCGCTAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaatttagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgattcatattacac                                                                                                                                    <  2:282108‑M1/250‑119 (MQ=255)                                                                                                          ttGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatt                                                                                                                          <  2:94975‑M1/250‑129 (MQ=255)                                                                                                                             ctGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatattt                                                                                                        <  1:388310‑M1/249‑147 (MQ=255)                                                                                                                               gTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttg                                                                                                       <  2:128355‑M1/248‑148 (MQ=255)                                                                                                                                              gTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaat                                                                                                                                                                                                                <  2:214028‑M1/128‑43 (MQ=255)                                                                                                                                              gTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaat                                                                                                                                                                                                                >  1:214028‑M1/1‑86 (MQ=255)                                                                                                                                                 cggcggTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatc                                                                                  <  1:357597‑M1/251‑169 (MQ=255)                                                                                                                                                        tAATTGATCAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataatttatatttattgcatataaatatttgtgatctacaacacgccttatctattgc                                                                            <  1:56962‑M1/250‑175 (MQ=255)                                                                                                                                                                   ataaaGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaac                                                                <  1:408555‑M1/251‑187 (MQ=255)                                                                                                                                                                                      aTCGCCAATATAGTTATTAATCAATATCAGTNGTTTATCCGTCACAatgcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataa                                             <  2:370046‑M1/251‑206 (MQ=255)                                                                                                                                                                                         gCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaa                                           >  2:645166‑M1/1‑43 (MQ=255)                                                                                                                                                                                                               atCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatattacaacaaatttcaatacgcatatcgttatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgcctaatctattgcttgtccgctaacggacagatcaaggtaataaaaaaggtcgacgaagcgcccttgg                     <  2:403675‑M1/250‑230 (MQ=255)                                                                                                                                                                                                                              gTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctttttacccgaaatgctaat     <  1:80594‑M1/251‑246 (MQ=255)                                                                                                                                                                                                                              gTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctttttacccgaaatgctaat     <  1:446254‑M1/251‑246 (MQ=255)                                                                                                                                                                                                                                 acaaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctttttacccgaaatgctaattac  >  1:32356‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                         CAGACGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACACGTTTCACGCGCCCTTCCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACAAAGGCGATACGCTTAACCCTGCTTAACGAGAATGCACCCTCTTGTTTCATCGCCTTAACCGCATTGGCTGACTGAGCAAA  >  NC_000913/3077341‑3077815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑