Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,281,895	Δ27 bp	intergenic (+1205/‑55)	agaV → / → agaS	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component/putative galactosamine‑6‑phosphate deaminase/isomerase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3281895	3281921	27	56 [0]	[2] 59	agaV/agaS	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component/putative galactosamine‑6‑phosphate deaminase/isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3281894	0 (0.000)	56 (0.960)	53/478	0.1	100%	intergenic (+1204/‑82)	agaV/agaS	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component/putative galactosamine‑6‑phosphate deaminase/isomerase
	?	NC_000913	3281922 =	0 (0.000)					intergenic (+1232/‑54)	agaV/agaS	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component/putative galactosamine‑6‑phosphate deaminase/isomerase

CTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATT  >  NC_000913/3281677‑3282172                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                           cacttcccccgtaaggcctttctgtttctttcgttttgatctgtgcagcggtgtcggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAg                                                                                                                                                                                                                                                        <  2:261027‑M2/5‑1 (MQ=255)         ccgtaaggcctttctttttctttcgttttgatctgtgcagcggtgtcggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCg                                                                                                                                                                                                                                                >  2:46413‑M2/238‑250 (MQ=255)           gtaaggcctttctttttctttcgttttgatctgtgcagcggtgtcggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACa                                                                                                                                                                                                                                             <  1:514730‑M2/16‑1 (MQ=255)              aggcctttctttttccttcgttttgatctgtgcagcggtgacggctgcgacgctaccgcgtcttatccgtcctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcaccaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACg                                                                                                                                                                                                                                          >  2:49495‑M2/233‑251 (MQ=255)                             tttcgttttgatctgtgcagcggtgtcggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTtc                                                                                                                                                                                                                           >  2:258606‑M2/218‑251 (MQ=255)                                 gttttgatctgtgcagcggggtcggttgcgactctaacgcgtcttatccgacctacatttggtgaccgtaaggcctgataaagcgtttgcgccgcatccagcaatcccttttctttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACATCGCCCGACGTCAAGTtcatca                                                                                                                                                                                                                       <  2:212095‑M2/38‑1 (MQ=255)                                      gatctgtgcagcggtgtcggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACt                                                                                                                                                                                                                   <  1:322565‑M2/42‑1 (MQ=255)                                        tctgtgcagcggtgtcggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTa                                                                                                                                                                                                                  >  1:240513‑M2/207‑249 (MQ=255)                                           gtgcagcggtgtcggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcggttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGAt                                                                                                                                                                                                             <  1:398464‑M2/48‑1 (MQ=255)                                               agcggtgtcggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTg                                                                                                                                                                                                           >  2:29687‑M2/200‑249 (MQ=255)                                                        ggatgcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGcc                                                                                                                                                                                                  >  2:338218‑M2/191‑249 (MQ=255)                                                            gcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGaaa                                                                                                                                                                                             >  1:465605‑M2/187‑250 (MQ=255)                                                            gcgacgctaacgcgtcttatccgacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGaaa                                                                                                                                                                                             >  1:37707‑M2/187‑250 (MQ=255)                                                              gacgctaacgcgtcttatccgacctacagttggcgaccccaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAAtt                                                                                                                                                                                          >  2:401875‑M2/185‑251 (MQ=255)                                                                                  nacctacagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCcgccgc                                                                                                                                                                       >  2:314186‑M2/165‑250 (MQ=255)                                                                                       acagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCgg                                                                                                                                                                 >  2:96428‑M2/160‑251 (MQ=255)                                                                                        cagttggtgaccgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGt                                                                                                                                                                >  1:100826‑M2/159‑251 (MQ=255)                                                                                          gttggtgacagcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTAc                                                                                                                                                              >  2:244404‑M2/157‑251 (MQ=255)                                                                                                 accgcaaggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACt                                                                                                                                                       <  1:494133‑M2/102‑1 (MQ=255)                                                                                                       aggccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTgaaga                                                                                                                                                  >  1:308373‑M2/144‑250 (MQ=255)                                                                                                          ccggataaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAagag                                                                                                                                              <  2:399615‑M2/111‑1 (MQ=255)                                                                                                                aaagcgtttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGc                                                                                                                                        <  2:312583‑M2/117‑1 (MQ=255)                                                                                                                       ttgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcgtttttctcctgtgatctttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACACCGCCCGACGTCAAGTTCATCAGCCTAAGGATTGAGTTATGCCAGAAAATGTCACCCCTGCTGCCGCCGCAACCAGTACATGGACTGAAGAAGAGATCCGCCATCAg                                                                                                                                  <  2:489705‑M2/123‑1 (MQ=255)                                                                                                                        tgcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGcc                                                                                                                                >  1:407093‑M2/127‑251 (MQ=255)                                                                                                                         gcgccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttctttttgctccattgaactttcagtgtcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGcc                                                                                                                                <  2:256164‑M2/125‑1 (MQ=255)                                                                                                                         gcgccgcatccagcaatcccttttgcttcctttatcttgtctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTTCACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCt                                                                                                                               <  1:247634‑M2/126‑1 (MQ=255)                                                                                                                            ccgcatccagcaatcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTcgc                                                                                                                            >  1:153935‑M2/123‑251 (MQ=255)                                                                                                                                      caatcccttttgcttccgttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTTCATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATcc                                                                                                                  <  2:465605‑M2/139‑1 (MQ=255)                                                                                                                                        atcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGt                                                                                                                >  1:317521‑M2/111‑251 (MQ=255)                                                                                                                                         tcccttttgcttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGtt                                                                                                               >  1:366840‑M2/110‑251 (MQ=255)                                                                                                                                                   ttcctttatcttttctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCa                                                                                                      >  1:95272‑M2/100‑250 (MQ=255)                                                                                                                                                                tctttcaacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCt                                                                                         <  1:447772‑M2/164‑1 (MQ=255)                                                                                                                                                                     caacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGtt                                                                                    <  1:96428‑M2/169‑1 (MQ=255)                                                                                                                                                                     caacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTc                                                                                   >  2:167161‑M2/82‑251 (MQ=255)                                                                                                                                                                      aacgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGATGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCAACGTTcc                                                                                  >  2:39270‑M2/81‑251 (MQ=255)                                                                                                                                                                       acgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCcg                                                                                 >  1:161553‑M2/80‑251 (MQ=255)                                                                                                                                                                       acgatcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCcg                                                                                 >  2:29772‑M2/80‑251 (MQ=255)                                                                                                                                                                          atcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCgcgc                                                                              >  1:68181‑M2/77‑251 (MQ=255)                                                                                                                                                                           tcacaaatttcgttttatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCt                                                                             >  2:7886‑M2/76‑251 (MQ=255)                                                                                                                                                                                 atttcgttttatttcttttttctccattgaactttcagttacttttctatagattttattcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTAAATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTAcgttccgcgcgaaata                                                                        >  2:360074‑M2/70‑244 (MQ=255)                                                                                                                                                                                          tatttcttttttctccattgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTg                                                               <  1:314186‑M2/190‑1 (MQ=255)                                                                                                                                                                                                          attgaactttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCaa                                               >  1:2983‑M2/45‑250 (MQ=255)                                                                                                                                                                                                            tgaacgttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAag                                             <  2:240513‑M2/208‑1 (MQ=255)                                                                                                                                                                                                               actttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAACGCGACAACGCCCGAAGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGCGATCCGCCATCAGCCTCGCGCATCGTTCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATACCTTCCTTGAACCGTTACTgcgcaaaacga                                          >  2:110367‑M2/40‑246 (MQ=255)                                                                                                                                                                                                                  ttcagtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACCCCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATCACTTCCTTGAACCGTTACTGCGCAAAGAGAATCt                                      >  2:41574‑M2/37‑251 (MQ=255)                                                                                                                                                                                                                    cagtttcttttctatagattttactcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGc                                    <  1:466178‑M2/217‑1 (MQ=255)                                                                                                                                                                                                                      gtttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGGTTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCCACGCGCTACGTTCCGCGCTCAATAACATCCTTGAACCGTTACGGCGCAAAGAGAATCTCCgg                                  >  2:195934‑M2/33‑251 (MQ=255)                                                                                                                                                                                                                       tttcttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCgg                                  >  2:252440‑M2/32‑250 (MQ=255)                                                                                                                                                                                                                        ttcttttctatagattttaatcaccgacagGCAAGTGAAAGCGACAACGCCCGACGTCCAGTTCATCAGACTAAGGCTTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCGACCGGTACATGGACTGAAGAAGAGATCCGCCCTCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGCCGCGCTACGTTCCGCGCTCAATACCTTCCTTGAACCGTTACTGCGCAAAGAGCATCTGCGGat                                >  2:192408‑M2/31‑251 (MQ=255)                                                                                                                                                                                                                           ttttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGatcat                             >  1:150792‑M2/28‑251 (MQ=255)                                                                                                                                                                                                                            tttctatagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGatc                               <  1:297052‑M2/222‑1 (MQ=255)                                                                                                                                                                                                                                 atagattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGATCATCCTGAc                       >  2:356186‑M2/22‑251 (MQ=255)                                                                                                                                                                                                                                    gattttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGATCATCCTGACCgg                    >  1:353160‑M2/19‑251 (MQ=255)                                                                                                                                                                                                                                        ttaatcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGATCATCCTGACCGGAGc                 <  1:338218‑M2/236‑1 (MQ=255)                                                                                                                                                                                                                                           atcaacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGATCATCCTGACCGGAGCCGGa             <  1:409747‑M2/240‑1 (MQ=255)                                                                                                                                                                                                                                             caacgaaagGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGATCATCCTGACCGGAGCCGGAAc           >  2:59357‑M2/10‑251 (MQ=255)                                                                                                                                                                                                                                                cGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAAAAGAGATCCGCCATCAGCCGCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACGTCCTTGAACCGTTTCTGCGCAAAGAGAATCTTCGGATCATCCTGACCGGAGCCGGAACGTc        <  1:192408/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aaaGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGATCATCCTGACCGGAGCCGGAACGTCgg      >  1:522938/1‑251 (MQ=255)                                                                                                                                                                                                                                                      gCAAGAGAAAGCGACAGCGCCCGACGTGAAGTTCATCAGACTAAGGATTGAGTTATGCCAGACAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAACTCGGCCATCAGCCTCGCGCATGGAGCCGTTCACTCACCAACATCTACGCGCTACGTTCCGCGCTCAATACCTTCCTTGAACCGTTACTGCGCAAAGAGAATCTACGGATCATCCTGACCTGAGCCGGCACGTCGGCAtt  >  2:202086/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                           CTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATT  >  NC_000913/3281677‑3282172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑