Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,281,895	Δ27 bp	intergenic (+1205/‑55)	agaV → / → agaS	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component/putative galactosamine‑6‑phosphate deaminase/isomerase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3281895	3281921	27	56 [0]	[2] 59	agaV/agaS	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component/putative galactosamine‑6‑phosphate deaminase/isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3281894	0 (0.000)	56 (0.960)	53/478	0.1	100%	intergenic (+1204/‑82)	agaV/agaS	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component/putative galactosamine‑6‑phosphate deaminase/isomerase
	?	NC_000913	3281922 =	0 (0.000)					intergenic (+1232/‑54)	agaV/agaS	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component/putative galactosamine‑6‑phosphate deaminase/isomerase

CACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCT  >  NC_000913/3281650‑3282136                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   cACTTCCCCCGTAAGGCCTTTCTGTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaag                                                                                                                                                                                                                                               <  2:261027‑M1/250‑6 (MQ=255)         ccGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcg                                                                                                                                                                                                                                       >  2:46413‑M1/1‑237 (MQ=255)           gTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgaca                                                                                                                                                                                                                                    <  1:514730‑M1/251‑17 (MQ=255)              aGGCCTTTCTTTTTCCTTCGTTTTGATCTGTGCAGCGGTGACGGCTGCGACGCTACCGCGTCTTATCCGTCCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACCAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacg                                                                                                                                                                                                                                 >  2:49495‑M1/1‑232 (MQ=255)                             tttcGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttc                                                                                                                                                                                                                  >  2:258606‑M1/1‑217 (MQ=255)                                 gTTTTGATCTGTGCAGCGGGGTCGGTTGCGACTCTAACGCGTCTTATCCGACCTACATTTGGTGACCGTAAGGCCTGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTCTTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacatcgcccgacgtcaagttcatca                                                                                                                                                                                                              <  2:212095‑M1/251‑39 (MQ=255)                                      gATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagact                                                                                                                                                                                                          <  1:322565‑M1/250‑43 (MQ=255)                                        tCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagacta                                                                                                                                                                                                         >  1:240513‑M1/1‑206 (MQ=255)                                           gtgCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGGTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggat                                                                                                                                                                                                    <  1:398464‑M1/251‑49 (MQ=255)                                               aGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattg                                                                                                                                                                                                  >  2:29687‑M1/1‑199 (MQ=255)                                                        ggATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgcc                                                                                                                                                                                         >  2:338218‑M1/1‑190 (MQ=255)                                                            gCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaa                                                                                                                                                                                    >  1:465605‑M1/1‑186 (MQ=255)                                                            gCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaa                                                                                                                                                                                    >  1:37707‑M1/1‑186 (MQ=255)                                                              gACGCTAACGCGTCTTATCCGACCTACAGTTGGCGACCCCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaatt                                                                                                                                                                                 >  2:401875‑M1/1‑184 (MQ=255)                                                                                  nacCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgc                                                                                                                                                              >  2:314186‑M1/2‑164 (MQ=255)                                                                                       aCAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccgg                                                                                                                                                        >  2:96428‑M1/1‑159 (MQ=255)                                                                                        cAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggt                                                                                                                                                       >  1:100826‑M1/1‑158 (MQ=255)                                                                                          gTTGGTGACAGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtac                                                                                                                                                     >  2:244404‑M1/1‑156 (MQ=255)                                                                                                 aCCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggact                                                                                                                                              <  1:494133‑M1/251‑103 (MQ=255)                                                                                                       aGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaaga                                                                                                                                         >  1:308373‑M1/1‑143 (MQ=255)                                                                                                          ccGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagag                                                                                                                                     <  2:399615‑M1/251‑112 (MQ=255)                                                                                                                aaaGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgc                                                                                                                               <  2:312583‑M1/251‑118 (MQ=255)                                                                                                                       ttGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCGTTTTTCTCCTGTGATCTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaccgcccgacgtcaagttcatcagcctaaggattgagttatgccagaaaatgtcacccctgctgccgccgcaaccagtacatggactgaagaagagatccgccatcag                                                                                                                         <  2:489705‑M1/250‑124 (MQ=255)                                                                                                                        tGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcc                                                                                                                       >  1:407093‑M1/1‑126 (MQ=255)                                                                                                                         gcgcCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTGCTCCATTGAACTTTCAGTGTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcc                                                                                                                       <  2:256164‑M1/250‑126 (MQ=255)                                                                                                                         gcgcCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTGTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaatttcacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcct                                                                                                                      <  1:247634‑M1/251‑127 (MQ=255)                                                                                                                            ccgcATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgc                                                                                                                   >  1:153935‑M1/1‑122 (MQ=255)                                                                                                                                      cAATCCCTTTTGCTTCCGTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggttcatggactgaagaagagatccgccatcagcctcgcgcatggatcc                                                                                                         <  2:465605‑M1/251‑140 (MQ=255)                                                                                                                                        aTCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgt                                                                                                       >  1:317521‑M1/1‑110 (MQ=255)                                                                                                                                         tCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgtt                                                                                                      >  1:366840‑M1/1‑109 (MQ=255)                                                                                                                                                   ttCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcacca                                                                                             >  1:95272‑M1/1‑99 (MQ=255)                                                                                                                                                                tctttcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgct                                                                                <  1:447772‑M1/250‑165 (MQ=255)                                                                                                                                                                     cAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttc                                                                          >  2:167161‑M1/1‑81 (MQ=255)                                                                                                                                                                     cAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgtt                                                                           <  1:96428‑M1/250‑170 (MQ=255)                                                                                                                                                                      aaCGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgatgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgcaacgttcc                                                                         >  2:39270‑M1/1‑80 (MQ=255)                                                                                                                                                                       aCGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccg                                                                        >  2:29772‑M1/1‑79 (MQ=255)                                                                                                                                                                       aCGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccg                                                                        >  1:161553‑M1/1‑79 (MQ=255)                                                                                                                                                                          aTCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgc                                                                     >  1:68181‑M1/1‑76 (MQ=255)                                                                                                                                                                           tCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgct                                                                    >  2:7886‑M1/1‑75 (MQ=255)                                                                                                                                                                                 aTTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTACTTTTCTATAGATTTTATTCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtaaatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgcgaaata                                                               >  2:360074‑M1/1‑69 (MQ=255)                                                                                                                                                                                          tATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttg                                                      <  1:314186‑M1/250‑191 (MQ=255)                                                                                                                                                                                                          aTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaa                                      >  1:2983‑M1/1‑44 (MQ=255)                                                                                                                                                                                                            tGAACGTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaag                                    <  2:240513‑M1/250‑209 (MQ=255)                                                                                                                                                                                                               aCTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaacgcgacaacgcccgaagtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagcgatccgccatcagcctcgcgcatcgttccgttcactcaccaacatcgacgcgctacgttccgcgctcaataccttccttgaaccgttactgcgcaaaacga                                 >  2:110367‑M1/1‑39 (MQ=255)                                                                                                                                                                                                                  ttCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacccccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaatcacttccttgaaccgttactgcgcaaagagaatct                             >  2:41574‑M1/1‑36 (MQ=255)                                                                                                                                                                                                                    cAGTTTCTTTTCTATAGATTTTACTCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgc                           <  1:466178‑M1/251‑218 (MQ=255)                                                                                                                                                                                                                      gTTTCTTTTCTATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaagggttgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatccacgcgctacgttccgcgctcaataacatccttgaaccgttacggcgcaaagagaatctccgg                         >  2:195934‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                       tttcttttctATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgcgg                         >  2:252440‑M1/1‑31 (MQ=255)                                                                                                                                                                                                                        ttcttttctATAGATTTTAATCACCGACAGgcaagtgaaagcgacaacgcccgacgtccagttcatcagactaaggcttgagttatgccagaaaattacacccctgctgccgccgcgaccggtacatggactgaagaagagatccgccctcagcctcgcgcatggatccgttcactcaccaacatcgccgcgctacgttccgcgctcaataccttccttgaaccgttactgcgcaaagagcatctgcggat                       >  2:192408‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                           ttttctATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgcggatcat                    >  1:150792‑M1/1‑27 (MQ=255)                                                                                                                                                                                                                            tttctATAGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgcggatc                      <  1:297052‑M1/248‑223 (MQ=255)                                                                                                                                                                                                                                 ataGATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgcggatcatcctgac              >  2:356186‑M1/1‑21 (MQ=255)                                                                                                                                                                                                                                    gATTTTAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgcggatcatcctgaccgg           >  1:353160‑M1/1‑18 (MQ=255)                                                                                                                                                                                                                                        ttAATCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgcggatcatcctgaccggagc        <  1:338218‑M1/250‑237 (MQ=255)                                                                                                                                                                                                                                           aTCAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgcggatcatcctgaccggagccgga    <  1:409747‑M1/251‑241 (MQ=255)                                                                                                                                                                                                                                             cAACGAAAGgcaagtgaaagcgacaacgcccgacgtcaagttcatcagactaaggattgagttatgccagaaaattacacccctgctgccgccgcaaccggtacatggactgaagaagagatccgccatcagcctcgcgcatggatccgttcactcaccaacatcgacgcgctacgttccgcgctcaataacttccttgaaccgttactgcgcaaagagaatctgcggatcatcctgaccggagccggaac  >  2:59357‑M1/1‑9 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   CACTTCCCCCGTAAGGCCTTTCTTTTTCTTTCGTTTTGATCTGTGCAGCGGTGTCGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATGGATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATCT  >  NC_000913/3281650‑3282136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑