Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,139,771	10483 bp→TG		[ptsA]–[eptC]	[ptsA], frwC, frwB, pflD, pflC, frwD, yijO, [eptC]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4139771	4150253	10483	66 [0]	[0] 67	[ptsA]–[eptC]	[ptsA],frwC,frwB,pflD,pflC,frwD,yijO,[eptC]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4139770	0 (0.000)	65 (0.690) +TG	60/484	0.4	100%	coding (2452/2502 nt)	ptsA	putative PTS multiphosphoryl transfer protein PtsA
	?	NC_000913	4150254 =	0 (0.000)					coding (12/1734 nt)	eptC	phosphoethanolamine transferase EptC

GGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4139522‑4139770 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGCAATCCCGGCAATAGTGACCATTAACGCTTG  >  NC_000913/4150254‑4150503                                                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                                           GGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTG                                                                                                                                                                                                                                                            >  2:838532/1‑251  GCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGT                                                                                                                                                                                                                                                           >  2:671187/1‑251     CATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGCGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTG                                                                                                                                                                                                                                                        <  1:547759/251‑1         GTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAAT                                                                                                                                                                                                                                                    <  2:533160/251‑1             GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCAT                                                                                                                                                                                                                                                <  2:445538/251‑1             GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCA                                                                                                                                                                                                                                                 <  1:140109/250‑1             GAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATACGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTATTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCAT                                                                                                                                                                                                                                                <  2:661597/251‑1                CAAACAGCATGCCGTCATCACCAATCGCTTTTTGCAGACGCGTCAGCACTTCCCATATGTACTCCTTGCGGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCAATAGAGTCGGCAGAGTCTGTGTGGAATGCATAGT                                                                                                                                                                                                                                             <  2:45996/251‑1                 AAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGCTACAGTTCCATGATGTTTCCTTTTACAGTTCCCGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGCGTGGAATGCATAGTt                                                                                                                                                                                                                                            >  2:306331/1‑250                     AGAATGCCCGCATCACCAATCGCTTTTTGCAGACGCGGCAGAACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGGTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCGTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAA                                                                                                                                                                                                                                        <  1:727057/251‑1                      GAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAAC                                                                                                                                                                                                                                       <  1:415041/251‑1                               CATCACCACTCGCTTTTTGCAGACGCGGCAGCACTTCCCATAGGGACTCCTGGCTGGCAGAGATAATGCTCGGGTGAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTG                                                                                                                                                                                                                               <  2:20630/250‑1                                  CACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAA                                                                                                                                                                                                                            >  2:70554/1‑250                                     CAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTACCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGACCCTGTCTTGAAAAAAT                                                                                                                                                                                                                        <  1:288999/251‑1                                          GCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGC                                                                                                                                                                                                                   >  1:780230/1‑251                                             TTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCG                                                                                                                                                                                                                 <  1:838532/250‑1                                                     gcgctGCAGCACTTCCCAGATGCACTCATAGCGGGCCGCGATAATGCTCGGGTTAGTTGGCACCCCGGCAATGGGGAAGATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCAGGATGTTTCCTGTGACAGTTCCAGTTCAGGTTGCAGCTGGCTGGCTATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTCAAAAAATATCTCCGAATGTAACG                                                                                                                                                                                                        <  2:718676/246‑1                                                            AGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCTCCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTC                                                                                                                                                                                                 <  1:297147/251‑1                                                               ACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTT                                                                                                                                                                                              <  1:450862/251‑1                                                                    CCATATGGACTCCTTTCTGGCAGCGATAATGCTCGGGTTAGTTGTCCACCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTTCAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGA                                                                                                                                                                                         <  2:586946/251‑1                                                                        ATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATC                                                                                                                                                                                     >  2:76346/1‑251                                                                           GACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTG                                                                                                                                                                                   >  1:671031/1‑250                                                                           GACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTG                                                                                                                                                                                   >  1:301132/1‑250                                                                                   GCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATAT                                                                                                                                                                          <  1:268109/251‑1                                                                                            GATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATAGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTT                                                                                                                                                                 >  1:90018/1‑251                                                                                             ATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTG                                                                                                                                                                <  2:401509/251‑1                                                                                               AATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGGTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGA                                                                                                                                                               <  2:701746/250‑1                                                                                                ATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAAT                                                                                                                                                             <  1:492355/251‑1                                                                                                        GTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGC                                                                                                                                                      >  2:742157/1‑250                                                                                                           agaTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACACTTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGAC                                                                                                                                                  >  2:793206/4‑251                                                                                                           AGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGAC                                                                                                                                                  >  1:246515/1‑251                                                                                                                 CACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAAT                                                                                                                                            <  2:777439/251‑1                                                                                                                 CACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAAT                                                                                                                                            >  1:119173/1‑251                                                                                                                      CGGCAATGGGGAAGATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATT                                                                                                                                       <  2:203828/251‑1                                                                                                                       GGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTG                                                                                                                                      >  1:166461/1‑251                                                                                                                             GGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAGTTCCTTAGGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGGACTATATTGAAAACG                                                                                                                                >  2:573672/1‑251                                                                                                                               GGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAG                                                                                                                              <  2:90985/251‑1                                                                                                                                GAATATGCGTGCAAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGATATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGG                                                                                                                             >  2:817571/1‑251                                                                                                                                  ATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGT                                                                                                                           >  1:204810/1‑251                                                                                                                                    ATGCGGGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAACCGAGGGTG                                                                                                                          <  1:257013/250‑1                                                                                                                                           CCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACA                                                                                                                  <  2:521516/251‑1                                                                                                                                               ACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAG                                                                                                              <  2:671820/251‑1                                                                                                                                                    CGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTT                                                                                                         >  1:798675/1‑251                                                                                                                                                      ACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTACATGATGTTTCCTTTTACAGTTCAAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTC                                                                                                        >  1:230037/1‑250                                                                                                                                                           TGCGACGTTGGCGGTGTCCAGATCCAGTTCCATGATGTTTCCTTTTTCAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGGAAGATCCTGTCTTGAAAAAAGATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTCTTTCgtaaa                                                                                                   <  2:250575/250‑6                                                                                                                                                             CGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATGGAAAGCGAGGGTGTTAGAACAGAAGTATTTCAGAAAACC                                                                                                <  2:613203/251‑1                                                                                                                                                               ACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTGCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGATGCGTGGAATGCATAGAAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGCTATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCT                                                                                              >  1:708324/1‑251                                                                                                                                                                 GTTAGCGGTGTCCAGATACAGTTCAATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGAAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCG                                                                                            >  1:252204/1‑251                                                                                                                                                                   tatCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGACTAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCACGC                                                                                           <  2:96984/247‑1                                                                                                                                                                            CCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCAC                                                                                 <  2:163670/251‑1                                                                                                                                                                                     GTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGA                                                                                                                                     >  2:846213/1‑190                                                                                                                                                                                     GTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGA                                                                                                                                     <  1:846213/190‑1                                                                                                                                                                                          ATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAA                                                                   <  2:491171/251‑1                                                                                                                                                                                          ATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGA                                                                    >  1:564837/1‑250                                                                                                                                                                                           gGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGGATTGCAGAAAACCCTCGCGCAAAATCACTAGGGTTTGCAGAAG                                                                  <  2:302569/250‑1                                                                                                                                                                                           TGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGA                                                                    >  1:647846/1‑249                                                                                                                                                                                                                CAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTAC                                             <  2:791662/251‑1                                                                                                                                                                                                                 AGTTCCTGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACG                                            >  1:802951/1‑251                                                                                                                                                                                                                             AGCAGGGTGGCGATAGCTACGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATGCAGAATTTGAATAAAGTGCAGACATAAATATATAGAAAACGAGGTTGTTATAACAGAAGAATTTCAGAAACCCCTCGCACAAAAGCACGAGGGTTGGCAGAAGAGGAAGAGTAGCTGTTATTACGGATCCCTGCagc                                <  2:34486/251‑4                                                                                                                                                                                                                             AGCAGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGC                                >  2:846836/1‑251                                                                                                                                                                                                                                AGGCTGGCGATAGCGTCGGCAGAGGCTGTGTGGCATGCATAGTAAGAACCTGCTTTGAAAAAATATCGCAGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTTAAAACGAGGGGGTTAGAACAGAAGTATTTCAGAAAACCCTCACGCAAAATCACGTGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGCAAT                             >  2:397594/1‑251                                                                                                                                                                                                                                 GGCTGGCGATAGCGTCGGCAGAGGCTGTGGGGAATGCATAGTAAGAACCTGGCTTGAAAAAATATCGCCGAATGTAACGACAATACATTAAGGATATCTGAAGGTATATCCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAACCCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGAATTACGCATCCCTGCCGCAATC                            <  2:14043/251‑1                                                                                                                                                                                                                                     ggctATAGCGGCGGCAGAGGCTGTGTGAAATGCATAGTAAGACCCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAAAGAGGGTGTTAGAACAGAAGTATTTCAGAAACCCCTCGCGCAAAAGCCCGAGGGTTTGCAGAAGAGGAATATTAGCCGGTATTCCCCATCCCTGCCGCAATCCCGG                        <  2:564837/247‑1                                                                                                                                                                                                                                            GCGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGCAATCCCGGCAATAGT                 <  1:167373/251‑1                                                                                                                                                                                                                                             CGTCGGCAGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAACCCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGCAATCCCGGCAATAGTG                <  2:739118/251‑1                                                                                                                                                                                                                                                    AGAGGCTGTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGCAATCCCGGCAATAGTGACCATT          >  2:425306/1‑250                                                                                                                                                                                                                                                           GTGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGCAATCCCGGCAATAGTGACCATTAACGCTT   <  2:780230/250‑1                                                                                                                                                                                                                                                           GTGTGGAATGCATAGAAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGCGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGCAATCCCGGCAATAGTGACCATTAACGCTTG  >  1:25202/1‑251                                                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                                           GGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4139522‑4139770 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTGGAATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGCGCAAAAGCACGAGGGTTTGCAGAAGAGGAAGATTAGCCGGTATTACGCATACCTGCCGCAATCCCGGCAATAGTGACCATTAACGCTTG  >  NC_000913/4150254‑4150503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.