Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,927,155	Δ12,222 bp		[asnA]–[hsrA]	[asnA], viaA, ravA, kup, rbsD, rbsA, rbsC, rbsB, rbsZ, rbsK, rbsR, [hsrA]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3927155	3939376	12222	30 [0]	[0] 31	[asnA]–[hsrA]	[asnA],viaA,ravA,kup,rbsD,rbsA,rbsC,rbsB,rbsZ,rbsK,rbsR,[hsrA]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3927154	0 (0.000)	30 (0.610)	28/490	0.6	100%	intergenic (‑151/‑1)	asnC/asnA	DNA‑binding transcriptional dual regulator AsnC/asparagine synthetase A
	?	NC_000913	3939377 =	0 (0.000)					coding (1236/1428 nt)	hsrA	putative transporter HsrA

CTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTA  >  NC_000913/3939136‑3939621                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     tttcgccagttcggcgtaagcggtgcgcgcattgcccattaatgcttccaggatgccacggtccagattgtcgatcagataattttccataggattttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACtt                                                                                                                                                                                                                                             >  1:1155‑M2/242‑251 (MQ=255)        agttcggcgtaagcggtgcgcgcattgcccattaatgcttccaggatgccacggtccagattgtcgatcagataattttccataggattttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCa                                                                                                                                                                                                                                       >  1:193921‑M2/235‑250 (MQ=255)                               attgcccattaatgcttccaggatgccacggaccagattgtcgatcagataattttccataggattttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTtgc                                                                                                                                                                                                               >  1:273617‑M2/212‑251 (MQ=255)                                 tgcccattaatgcttccaggatgccacggtccagattgtcgatcagataattttccataggattttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTtgct                                                                                                                                                                                                              <  2:1155‑M2/41‑1 (MQ=255)                                        taatgcttccaggatgccacggtccagattgtcgatcagataattttccataggattttcttatgctgattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTgcgttac                                                                                                                                                                                                       <  2:403347‑M2/48‑4 (MQ=255)                                         aatgcttccaggatgccacggtccagattgtcgatcagataattttccataggattttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCg                                                                                                                                                                                                     >  1:3762‑M2/202‑251 (MQ=255)                                                               ccagattgtcgatcagataattttccataggattttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCACCGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCgctgct                                                                                                                                                                               <  1:64136‑M2/72‑1 (MQ=255)                                                                                taattttccataggattttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGATACCGCTGCTGGCATTGTCATCGGTc                                                                                                                                                               >  2:404447‑M2/163‑250 (MQ=255)                                                                                     ttccataggattttcttatgcggattgatgattcattctattttagccttcttgtttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATc                                                                                                                                                          <  2:96380‑M2/93‑1 (MQ=255)                                                                                             gattttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagagagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTCATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCGGGCATTGTCCTCGGGCAGATCGGAAagtgg                                                                                                                                                 >  2:239596‑M2/150‑250 (MQ=255)                                                                                                 ttcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGAt                                                                                                                                              <  1:153785‑M2/105‑1 (MQ=255)                                                                                                  tcttatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggcgtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGCGATgg                                                                                                                                            >  2:386948‑M2/145‑251 (MQ=255)                                                                                                     tatgcggattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGt                                                                                                                                           <  2:15965‑M2/108‑1 (MQ=255)                                                                                                           gattgatgattcattctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATcg                                                                                                                                   <  1:164333‑M2/116‑1 (MQ=255)                                                                                                                          ctattttagccttcttttttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGtcc                                                                                                                    >  1:32645‑M2/121‑250 (MQ=255)                                                                                                                                            ttaatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAat                                                                                                   >  2:177748‑M2/103‑250 (MQ=255)                                                                                                                                              aatgaatcaaaagtgagttaggctttttattgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAatat                                                                                                 >  1:244930‑M2/101‑250 (MQ=255)                                                                                                                                                                          attgaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAg                                                                    <  2:278453‑M2/179‑1 (MQ=255)                                                                                                                                                                             gaatgattattgcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCa                                                                 <  1:177748‑M2/182‑1 (MQ=255)                                                                                                                                                                                        gcatgtgtgtcggtttttgttgcttaatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGa                                                       <  1:197545‑M2/192‑1 (MQ=255)                                                                                                                                                                                                  cggtttttgttgcttaagcataagcaacaggacgcaggagtataaaaaGGCCGCCCTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGATGGTGATTGCAAAgaga                                            <  2:422942‑M2/203‑1 (MQ=255)                                                                                                                                                                                                   ggtttttgttgcttaatcataagcaccaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAgaga                                            >  1:111780‑M2/48‑250 (MQ=255)                                                                                                                                                                                                                 aatcataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTGGTTGCGTGGCCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGAAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTa                             <  1:232805‑M2/218‑1 (MQ=255)                                                                                                                                                                                                                    cataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGc                           <  2:343747‑M2/220‑1 (MQ=255)                                                                                                                                                                                                                     ataagcaacaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGccc                         >  1:61121‑M2/30‑251 (MQ=255)                                                                                                                                                                                                                            acaggacgcaggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATc                  <  1:404447‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                               ggacgccggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCAcc               >  2:117553‑M2/20‑251 (MQ=255)                                                                                                                                                                                                                                    caggagtataaaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGAt          >  2:123987‑M2/15‑251 (MQ=255)                                                                                                                                                                                                                                         gtataaaaaGGCCGCACTGACAGCACCGCATAAGTTAATCGACATTTGCTGCTTGACCGCCAGTACACTGTTACCGCTGCTGTGATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGGCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATTTAGCCATTGCTGGTGATTGCAAATAGAACTGAGCGATCATTAGCCCAATAATCAGCGTGATCCCCa     <  2:271669‑M2/242‑1 (MQ=255)                                                                                                                                                                                                                                             caaaaGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACCCTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCACGTGTGATGGTATTCATCGCGGTACACTGCGTCGACATAGCCCTCCCTAATATAAACAACGGCAAGCTCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTa  >  2:173978‑M2/6‑250 (MQ=255)                                                                                                                                                                                                                                             aaaaaggCCGCACTTCCACCCACGCCTAAACGAATCGAAAGATCCTGCGTGACCGCCCGAACACTGTGACCGCTACAGGCATTGTCATCGGCCAGCTCGGCAAGTGAGATGGTATTAATCTAGGCAAACTGCGTCGACATAGCCATACCTAATATAAACAACAGCAAGCTCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTa  >  2:326987/6‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCATTCCTTCATAAACGCGAAGGACGGCCGCACTTACAGCAACGCCTAAACTAATCGACAGTTGCTGCGTGACCGCCAGAACACTGTTACCGCTGCTGGCATTGTCATCGGTCAGATCGGCAAGTGTGATGGTATTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTA  >  NC_000913/3939136‑3939621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.