Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,927,155	Δ12,222 bp		[asnA]–[hsrA]	[asnA], viaA, ravA, kup, rbsD, rbsA, rbsC, rbsB, rbsZ, rbsK, rbsR, [hsrA]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3927155	3939376	12222	30 [0]	[0] 31	[asnA]–[hsrA]	[asnA],viaA,ravA,kup,rbsD,rbsA,rbsC,rbsB,rbsZ,rbsK,rbsR,[hsrA]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3927154	0 (0.000)	30 (0.610)	28/490	0.6	100%	intergenic (‑151/‑1)	asnC/asnA	DNA‑binding transcriptional dual regulator AsnC/asparagine synthetase A
	?	NC_000913	3939377 =	0 (0.000)					coding (1236/1428 nt)	hsrA	putative transporter HsrA

TTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGG  >  NC_000913/3926914‑3927399                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      tttCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcactt                                                                                                                                                                                                                                             >  1:1155‑M1/1‑241 (MQ=255)        aGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagca                                                                                                                                                                                                                                       >  1:193921‑M1/1‑234 (MQ=255)                               aTTGCCCATTAATGCTTCCAGGATGCCACGGACCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgc                                                                                                                                                                                                               >  1:273617‑M1/1‑211 (MQ=255)                                 tGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgct                                                                                                                                                                                                              <  2:1155‑M1/250‑42 (MQ=255)                                        tAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCTGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgttac                                                                                                                                                                                                       <  2:403347‑M1/250‑49 (MQ=255)                                         aaTGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccg                                                                                                                                                                                                     >  1:3762‑M1/1‑201 (MQ=255)                                                               ccAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaccgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgct                                                                                                                                                                               <  1:64136‑M1/251‑73 (MQ=255)                                                                                tAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgataccgctgctggcattgtcatcggtc                                                                                                                                                               >  2:404447‑M1/1‑162 (MQ=255)                                                                                     ttCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTGTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatc                                                                                                                                                          <  2:96380‑M1/250‑94 (MQ=255)                                                                                             gATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGAGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactcatcgacagttgctgcgtgaccgccagaacactgttaccgctgcgggcattgtcctcgggcagatcggaaagtgg                                                                                                                                                 >  2:239596‑M1/1‑149 (MQ=255)                                                                                                 ttCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgat                                                                                                                                              <  1:153785‑M1/250‑106 (MQ=255)                                                                                                  tCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGCGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgcgatgg                                                                                                                                            >  2:386948‑M1/1‑144 (MQ=255)                                                                                                     tATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggt                                                                                                                                           <  2:15965‑M1/249‑109 (MQ=255)                                                                                                           gATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcg                                                                                                                                   <  1:164333‑M1/251‑117 (MQ=255)                                                                                                                          cTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcc                                                                                                                    >  1:32645‑M1/1‑120 (MQ=255)                                                                                                                                            ttAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaat                                                                                                   >  2:177748‑M1/1‑102 (MQ=255)                                                                                                                                              aaTGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatat                                                                                                 >  1:244930‑M1/1‑100 (MQ=255)                                                                                                                                                                          aTTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatag                                                                    <  2:278453‑M1/251‑180 (MQ=255)                                                                                                                                                                             gAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagcca                                                                 <  1:177748‑M1/251‑183 (MQ=255)                                                                                                                                                                                        gCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagccattgctggtga                                                       <  1:197545‑M1/250‑193 (MQ=255)                                                                                                                                                                                                  cGGTTTTTGTTGCTTAAGCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcccttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagccattgatggtgattgcaaagaga                                            <  2:422942‑M1/251‑204 (MQ=255)                                                                                                                                                                                                   ggTTTTTGTTGCTTAATCATAAGCACCAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagccattgctggtgattgcaaagaga                                            >  1:111780‑M1/1‑47 (MQ=255)                                                                                                                                                                                                                 aaTCATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagtggttgcgtggccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggaaagatcagcatccatatagccattgctggtgattgcaaagagaactgagcgatcatta                             <  1:232805‑M1/251‑219 (MQ=255)                                                                                                                                                                                                                    cATAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagccattgctggtgattgcaaagagaactgagcgatcattagc                           <  2:343747‑M1/250‑221 (MQ=255)                                                                                                                                                                                                                     aTAAGCAACAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagccattgctggtgattgcaaagagaactgagcgatcattagccc                         >  1:61121‑M1/1‑29 (MQ=255)                                                                                                                                                                                                                            aCAGGACGCAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagccattgctggtgattgcaaagagaactgagcgatcattagcccaataatc                  <  1:404447‑M1/251‑230 (MQ=255)                                                                                                                                                                                                                               ggACGCCGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagccattgctggtgattgcaaagagaactgagcgatcattagcccaataatcacc               >  2:117553‑M1/1‑19 (MQ=255)                                                                                                                                                                                                                                    cAGGAGTATAAAAAggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaacactgttaccgctgctggcattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcgtcgacatagccatccctaatataaacaacggcaagatcagcatccatatagccattgctggtgattgcaaagagaactgagcgatcattagcccaataatcaccgtgat          >  2:123987‑M1/1‑14 (MQ=255)                                                                                                                                                                                                                                         gTATAAAAAggccgcactgacagcaccgcataagttaatcgacatttgctgcttgaccgccagtacactgttaccgctgctgtgattgtcatcggtcagatcggcaagtgtgatggtattcatcgcggtaaactgcggcgacatagccatccctaatataaacaacggcaagatcagcatccatttagccattgctggtgattgcaaatagaactgagcgatcattagcccaataatcagcgtgatcccca     <  2:271669‑M1/251‑243 (MQ=255)                                                                                                                                                                                                                                             caaaaggccgcacttacagcaacgcctaaactaatcgacagttgctgcgtgaccgccagaaccctgttaccgctgctggcattgtcatcggtcagatcggcacgtgtgatggtattcatcgcggtacactgcgtcgacatagccctccctaatataaacaacggcaagctcagcatccatatagccattgctggtgattgcaaagagaactgagcgatcattagcccaataatcaccgtgatccccacta  >  2:173978‑M1/2‑5 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      TTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGG  >  NC_000913/3926914‑3927399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.