Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,707,484	IS3 (+) +3 bp	coding (220‑222/1608 nt)	dppA ←	dipeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2170171 =	NA (NA)	44 (0.840)	44/482	0.2	100%	noncoding (1/1258 nt)	IS3	repeat region
	?	NC_000913	= 3707486	0 (0.000)					coding (220/1608 nt)	dppA	dipeptide ABC transporter periplasmic binding protein
*	?	NC_000913	= 2171428	NA (NA)	35 (0.660)	34/486	0.4	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	3707484 =	0 (0.000)					coding (222/1608 nt)	dppA	dipeptide ABC transporter periplasmic binding protein

CAATACCATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGAC  >  NC_000913/2169923‑2170408                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              cAATACCATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTAGTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGa                                                                                                                                                                                                                                             <  2:237717/251‑1 (MQ=255)        aTCAGCACCGACAAACATTAAATCGGCATTAATATCCTGCCATGAACCCCCGGCAATGGAACCATGCATAGAGAGCGTTTCATCGCGGACCGCGCCACCCCCGTCGACCAGCGCAATGTCATTAATTTCGGCAAGGGCAAAAGCTGCCGGGCGACTGTTGGCAATCACCGTGCTATTTTTGGCGGTCATTAATCCTTCGGCGATCAGCATTGTCGTACTTCCGCTGTCGGGAATAACGGTCTGATCTTAcc                                                                                                                                                                                                                                      >  1:242471/1‑251 (MQ=255)            gCACCGACAAACATTAAATCGGCATTAATATCCTGTCATGAACCCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGCGCCACCAACGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATAACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTTTCGTACTTCCGCTGTCGCGAATAACGGTCTGATCTTACCCAGc                                                                                                                                                                                                                                  >  2:284520/1‑251 (MQ=255)            gCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGc                                                                                                                                                                                                                                  <  2:162429/251‑1 (MQ=255)              aCCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCa                                                                                                                                                                                                                                 >  1:245204/1‑250 (MQ=255)                 gACAAACATTAAATCGGCATTAATATCCTGTAATGAAAGCTCGGCAACGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGGCGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGACTTTTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAAt                                                                                                                                                                                                                               >  1:418315/1‑249 (MQ=255)                     aaCATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACCGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGCGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTTGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATTTTTCCCAGCAATAGTggg                                                                                                                                                                                                                         >  2:339028/1‑250 (MQ=255)                            aaTCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCCCCACAGACGACCAGCGGAATGACTTTATTTTCGGAAAGGGCCAACGCTGCCGGGAGAATGTTGGTAATCACCGTGATATTTTTGGCGGTCATTTATCCTTCGGCGATAAGCATTGTTGTACTTCCGCTGTCGGGAATAACGGTCTGGTCTTACCCAGCAATaagtgacacccgg                                                                                                                                                                                                                  >  2:59429/1‑239 (MQ=255)                                       atatCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGCGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGGGAGt                                                                                                                                                                                                        >  1:415500/1‑250 (MQ=255)                                       atatCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGCGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGt                                                                                                                                                                                                        >  1:117113/1‑250 (MQ=255)                                       atatCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTCATGGCGGACCGTGCCAACACCGAAGACCCGCGGAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGCCTGTTGGTAATCACCGTGATATTTTTGGCGGTCCTTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGGCGAGAATAAAGGTCTGATCTTACCCCGCAATAGGGGACCAGCGGCTAAGTGAgg                                                                                                                                                                                                        >  1:458521/1‑249 (MQ=255)                                           ccTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCAACACAGACGACCAGAGGAATGTTTTTATTTTCGGCAAGGGCAAACGCTGCCGGGAGTCTGTTGGCAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACCCGCGGCTAAGTGAGTAAAct                                                                                                                                                                                                   >  1:306273/1‑251 (MQ=255)                                                aaTGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGGAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGGAAACtctc                                                                                                                                                                                                >  2:407042/1‑249 (MQ=255)                                                        cTCGGCACTGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCCGTCAGAggg                                                                                                                                                                                      >  1:170166/1‑250 (MQ=255)                                                              aaTGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCAAAAACCGACGACCAGAGTCATGTCTTTATTTTCGGAAAGAGCAAACGCTGACGGGAGACTGTTGGTAATCACCGTGATCTTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGACTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGaaca                                                                                                                                                                                 >  1:35667/1‑247 (MQ=38)                                                                  gAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATg                                                                                                                                                                            <  1:264651/251‑1 (MQ=255)                                                                  gAACCATGCATCGAGCGCGTTTTACGGCGGACCGTGCCACCACAGACGACCAGAGTACTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCCGGCGCCCGTTGGTAATCACCGTGATATTTTTGGCGGTCATTACTCCCTCCGCGGTAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGGCTGATCTTACCCAGCAATACTGGACACGCGGCTAAGGGGGGAAAACCTCAGCCAGAGGTGACTcaccat                                                                                                                                                                            >  2:38099/1‑248 (MQ=255)                                                                   aaCCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTTATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGGAATTACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGGCGAGAATAACGGACTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCATAGGTGACTCACATg                                                                                                                                                                            >  1:259415/1‑250 (MQ=255)                                                                            tCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACAt                                                                                                                                                                             <  2:157944/240‑1 (MQ=255)                                                                            tCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACAt                                                                                                                                                                             >  1:157944/1‑240 (MQ=255)                                                                               aGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTAt                                                                                                                                                               <  1:391917/251‑1 (MQ=255)                                                                                                 ccGTGCCACCACAGACGACCAGAGTAATTTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCATCAATAGTGGACACGCTGCTAAGTGAGTAAACTCTCAGTCAGTGGTGTCTCACATGACAAAAACAGTATCAACCAGTAAAAAAcccc                                                                                                                                             <  1:63499/251‑1 (MQ=255)                                                                                                     gCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTa                                                                                                                                          >  1:147292/1‑250 (MQ=255)                                                                                                          cacaGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGGAAAAAACCCCGTAAACAGc                                                                                                                                    <  1:407042/251‑1 (MQ=255)                                                                                                           acaGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCa                                                                                                                                   <  1:422654/251‑1 (MQ=255)                                                                                                             aGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTGCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCAtt                                                                                                                                 <  1:59429/251‑1 (MQ=255)                                                                                                              gacgacCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGGAAAAAACCCCGTAAACAGCATTc                                                                                                                                <  2:420040/251‑1 (MQ=255)                                                                                                                 gacCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGcc                                                                                                                             >  1:375825/1‑251 (MQ=255)                                                                                                                 gaacAGAGAAATGTCTTTATTTTCGGACAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTACGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGcc                                                                                                                             >  1:375835/4‑251 (MQ=255)                                                                                                                     agagTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGaa                                                                                                                         <  2:259415/251‑1 (MQ=255)                                                                                                                        gTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAAttt                                                                                                                      >  1:248334/1‑251 (MQ=255)                                                                                                                          aaTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAg                                                                                                                                     <  1:215797/234‑1 (MQ=255)                                                                                                                          aaTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAg                                                                                                                                     >  2:215797/1‑234 (MQ=255)                                                                                                                                     tttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAAGCCTTCGGCGATAAGCATTGTCGTACTGCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAAGTTCGCAGTGAAGcc                                                                                                          <  2:306273/250‑1 (MQ=255)                                                                                                                                           aaaGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGCTATTTTTGGCGGTCATTAATCCTTCGGAGATAAGCATTGTCGTACTTCCGCTGTCGCGAATAACGGTCTGATCTTAACCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTAAGAGGTGACTCACATGACAACAACAGACTCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGa                                                                                                      >  1:464075/1‑248 (MQ=21)                                                                                                                                             aGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGCGCTATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACACCGTAAACAGCATTCGCCTGCATTTCGCAGTGAAGCCCTGAAGCtt                                                                                                 >  1:250529/1‑251 (MQ=255)                                                                                                                                               ggCAAACGCTGCCGGGAGACCGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGTCTCACATGACAAAAACAGTATCAACCAGGAAAAAACCCCGTAAACAGCATTCTCCTTAATGTCGCATTGAAGCCCGGAAGCTTGc                                                                                               <  2:458521/251‑1 (MQ=25)                                                                                                                                                gCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTGTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCt                                                                                              <  2:162563/251‑1 (MQ=255)                                                                                                                                                           cGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCgg                                                                                   >  1:106194/1‑251 (MQ=255)                                                                                                                                                                    gTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGc                                                                          <  2:375825/251‑1 (MQ=255)                                                                                                                                                                    gTTGGTAAGCTCCGTGATATTTTTGGCGGTCATGAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCGTGCTGAACGCATCGGTGTTACTGc                                                                          <  2:375835/251‑1 (MQ=255)                                                                                                                                                                                cGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGa                                                              <  2:245204/251‑1 (MQ=255)                                                                                                                                                                                    atatTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTc                                                          <  2:160687/251‑1 (MQ=255)                                                                                                                                                                                      atTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTc                                                          >  1:94563/1‑249 (MQ=255)                                                                                                                                                                                                     tAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATcac                                          >  1:96492/1‑250 (MQ=255)                                                                                                                                                                                                      aaTCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATcaca                                         <  2:170166/250‑1 (MQ=255)                                                                                                                                                                                                            tCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCCGAAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTAc                                  >  2:57165/1‑251 (MQ=255)                                                                                                                                                                                                             cGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTAc                                  >  2:225652/1‑250 (MQ=255)                                                                                                                                                                                                                        aTTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACATCTCTACAACTGGCGCAGt                      <  1:339028/251‑1 (MQ=255)                                                                                                                                                                                                                           gtcttacgaccGCTGTCGAGAATAACGGTCTGATCTTGCCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGGCAGAGGTGACTCACATGACAAATACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCTGTaa                    <  2:15013/241‑1 (MQ=11)                                                                                                                                                                                                                           gTCGTACTTCCGCTGGCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGATGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTCCTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTaa                    <  2:15000/250‑1 (MQ=255)                                                                                                                                                                                                                              gTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAAc                  >  2:163247/1‑249 (MQ=255)                                                                                                                                                                                                                                         gTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAATCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATcagca     <  2:415500/251‑1 (MQ=25)                                                                                                                                                                                                                                            gagaATAACGGTCTGATCTTACCCAGCACTAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGACTCACAACTCTCCAACTGGCGCAGTAAACAGCAAAATCAGCAGAc  >  1:394342/1‑251 (MQ=21)                                                                                                                                                                                                                                            gagaATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGAc  >  2:91088/1‑251 (MQ=34)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              CAATACCATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGAC  >  NC_000913/2169923‑2170408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.