Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 2,016,437 Δ2 bp :: IS186 (–) +8 bp :: Δ1 bp coding (570‑577/687 nt) fliH → flagellar biosynthesis protein FliH

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2016437 =0 (0.000)36 (1.180) 32/484 0.1 100% coding (570/687 nt) fliH flagellar biosynthesis protein FliH
?NC_000913 2514274 = NA (NA)noncoding (2/1345 nt) IS186 repeat region
* ? NC_000913 = 20164440 (0.000)45 (1.470) 43/486 0.0 100% coding (577/687 nt) fliH flagellar biosynthesis protein FliH
?NC_000913 = 2515615 NA (NA)noncoding (1343/1345 nt) IS186 repeat region

GAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCA  >  NC_000913/2515434‑2515862
                                                                                                                                                                                     |                                                                                                                                                                                                                                                       
gaagaaCTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGTGCGACCAATCAAATACCCTTg                                                                                                                                                                                    <  1:245293/251‑1 (MQ=255)
         actCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATGCCCTTGCAGATATTg                                                                                                                                                                           <  2:211413/251‑1 (MQ=255)
                       ataacaaatctgttcctCTGGAGCCTACCGGTGGACATTCGTGGAACCGTATCCCTTACACCCTCCCACACGCAATTTCATAATCCGCGGCATCGTCTTCACGCGGCACCGCGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTTTGGGATACTTCTCCGGTTTTTTTACGCCTGTTAATAAGAGATTGGCTGCGGGCGACCCATCAATTACCCTTGCAGTTATTGCACCCGGAGcgga                                                                                                                                                              >  2:195578/17‑247 (MQ=12)
                                                  cAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGtct                                                                                                                                  <  1:4288/251‑1 (MQ=255)
                                                     gTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGa                                                                                                                               <  1:46170/251‑1 (MQ=255)
                                                      tGGCCATTCGTGGGACAGTATCCCTGCCAGCCTACAAAACGAACTTGCAGAACGCGAGGCATCGTCTTAAAGAGGCACCGAGGCGTAGCCTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTATTCTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCGCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGAc                                                                                                                              >  2:129285/1‑251 (MQ=25)
                                                                 gggACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCcgcg                                                                                                                    <  1:91960/250‑1 (MQ=255)
                                                                      aGTATCCCTGACAGCCTACAAAACGCACTTGAAGAACGCGAGGCCTCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCACTAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACcacc                                                                                                              >  2:28647/1‑250 (MQ=255)
                                                                       gTATCCCTGACATCCTACAAAACGCAAGTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCccac                                                                                                             <  2:107358/251‑1 (MQ=255)
                                                                       gTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCacac                                                                                                             >  1:202083/1‑251 (MQ=255)
                                                                                aCAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACt                                                                                                    >  1:285614/1‑251 (MQ=255)
                                                                                              gCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGcc                                                                                         >  1:251754/1‑248 (MQ=255)
                                                                                                             gAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGAtctc                                                                       >  1:70194/1‑251 (MQ=255)
                                                                                                              aGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTAAGCAGGTTCAACCCTTAAGTTAGAGATTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGATGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTGACTTTCGCCAGATCGGTAATTGAtatc                                                                       >  1:266713/1‑247 (MQ=39)
                                                                                                               ggCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTcac                                                                     <  1:4441/251‑1 (MQ=255)
                                                                                                                gCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACCAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTcac                                                                     >  1:185422/1‑250 (MQ=255)
                                                                                                                 cATCGTCGTAACGAGGCAAGGAGGCGAAGCATTCGTCAGATGGCTCAACCCTTAGGTTAGCGCTTAAGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGAGACCAATCCAATACCCTTGTAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGGTGCGTCTCGACAACCTCCGCGACCACACTCAATGAATTCGCTTTCGCCAGATCGGTAATTGATCTcaca                                                                    >  2:190262/1‑250 (MQ=255)
                                                                                                                  aTCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACACTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATAAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGCTATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAat                                                                  >  1:287871/1‑251 (MQ=255)
                                                                                                                                            cGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGGTTTTTGACGCCTGTTAAGCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCTTCTCGACAAACTCCGCGACCACACTCAATGACGTCGCGTGCGCCAGATCGGTAATTGATCTCACAATCATCGCTTCCAGCGTGTTCGTGACaa                                        <  2:18841/251‑1 (MQ=255)
                                                                                                                                               aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGGTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAatat                                     <  2:91126/251‑1 (MQ=255)
                                                                                                                                                        aTGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGACTGTTAATCAGCTAATGGCTGCGGCCGGACAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCACTAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTAGCAAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTcaca                             >  1:129115/1‑250 (MQ=255)
                                                                                                                                                               aaCCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGcc                     <  2:202083/251‑1 (MQ=255)
                                                                                                                                                                      aaGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGGCTGATAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGAAATTGATCTCACAATCATCGCATACAGCGTGTTCGTGACAATATCTTTCACAAAGACGCAATCAAtt              >  2:75738/1‑251 (MQ=255)
                                                                                                                                                                                  aTGGGATAATTCCCCTGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCa  <  2:170341/251‑1 (MQ=255)
                                                                                                                                                                                  aTGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCATATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCATCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATc   <  1:129421/250‑1 (MQ=255)
                                                                                                                                                                                     |                                                                                                                                                                                                                                                       
GAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCA  >  NC_000913/2515434‑2515862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.