Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,707,484	+GA :: IS3 (+) +4 bp	coding (219‑222/1608 nt)	dppA ←	dipeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	19 (0.570) +TC	17/482	0.8	100%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	= 3707487	0 (0.000)					coding (219/1608 nt)	dppA	dipeptide ABC transporter periplasmic binding protein
*	?	NC_000913	= 2171428	NA (NA)	17 (0.500)	16/486	0.9	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	3707484 =	0 (0.000)					coding (222/1608 nt)	dppA	dipeptide ABC transporter periplasmic binding protein

GAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCT  >  NC_000913/314986‑315470                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  gAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAAAGCCCGGCGAATGGCTGGGATATTCGTGATGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCACGCCTGATGTATGAACAGTATTATGGCGATGACATCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGCGGTGACCTATACGCCAGTGCCTCTTAccc                                                                                                                                                                                                                                            >  2:304723/1‑251 (MQ=255)  aGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTAcccc                                                                                                                                                                                                                                           >  1:93545/1‑250 (MQ=255)                tatTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACTCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGAAGCTTGGCGAAAGCCTGATGTATGATCAGGATTATGGGGGTGAAGTCGGGCTGATTGGGACAGCTAACGGCCAGCAAGCACCGCAGGCTATTTCTGGCGATGTGCCCTATAAGCCAGTGCGTCTACCCCAGCactacaggcaac                                                                                                                                                                                                                             >  2:12356/1‑240 (MQ=255)                      ggCTTCTGGTTGAAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAAGGGCTGGGATATTCGGGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCt                                                                                                                                                                                                                       <  2:246430/251‑1 (MQ=255)                            tggctggAAACAATCGCCGGATATTGAGGATTATCAGGAAAGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTCTTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGACAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTg                                                                                                                                                                                                                  >  2:161276/1‑250 (MQ=255)                                   aaaaaaTCGCCGGATATTGAGGATTATCAGGAACGCCCGGAGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAAct                                                                                                                                                                                                          <  1:7406/251‑1 (MQ=255)                                    aaaaaTCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAActc                                                                                                                                                                                                         <  1:14962/251‑1 (MQ=255)                                              ggATATTGAGGCTTATCAGGAACGCCCGGCGCATGGCTGGGATATTCGTGCTGCGGGCTATTTACACGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACACGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGGGAGTAAACTCtcagtcag                                                                                                                                                                                                 >  2:144381/1‑249 (MQ=255)                                              gccgccagaaactttatggtacgggttttgcgcgtttttctgacgatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCtcagtcag                                                                                                                                                                                                 <  2:177744‑M1/51‑1 (MQ=255)                                                     gaaactttatggtacgggttttgcgcgtttttctgacgatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTc                                                                                                                                                                                        >  2:247868‑M1/192‑251 (MQ=255)                                                     gAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTc                                                                                                                                                                                        >  2:162695/1‑251 (MQ=255)                                                            aTCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGa                                                                                                                                                                                  <  1:103520/250‑1 (MQ=255)                                                                     gCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGACCAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGGGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGt                                                                                                                                                                        >  2:169337/1‑251 (MQ=255)                                                                     gCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGt                                                                                                                                                                        >  2:339531/1‑251 (MQ=255)                                                                            gAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTTATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCATCAATAGTGGACACGCGGCTAAGTTAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAAcc                                                                                                                                                                 <  1:144381/251‑1 (MQ=255)                                                                                tttttctgacgatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGt                                                                                                                                                              >  2:277361‑M1/165‑250 (MQ=255)                                                                                 gCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTaa                                                                                                                                                            <  1:162695/251‑1 (MQ=255)                                                                                   ttctgacgatcgaaatagaaccccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacctcccacttttcagcgaggcccgggatcactgaGCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTaaa                                                                                                                                                           >  2:179604‑M1/162‑250 (MQ=255)                                                                                          gatcgaacgagaacaccccatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCg                                                                                                                                                   <  1:282316‑M1/97‑1 (MQ=255)                                                                                          gatcgaacgagaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCg                                                                                                                                                   <  1:64389‑M1/97‑1 (MQ=255)                                                                                             gTGCTGAGGGCTATCTACCCGCATGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGCAGTCGGGCTGTTTGGTACAGATAAGCGCCAGAAAGACCCGCATGCAATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGGGACTCACATGCCACAAACAGTATCAACACGTAAAAAAcacctga                                                                                                                                                 >  2:87508/1‑244 (MQ=37)                                                                                                    gaacaccacatcatcggcgttcagttcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCAt                                                                                                                                         <  1:10138‑M1/107‑1 (MQ=255)                                                                                                      gctttttccccGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTc                                                                                                                                       <  2:126066/243‑1 (MQ=255)                                                                                                                  cggcgttcagttcacgcgtcggtttgaattctttattctcgtgccacttcaccactttacgcagatggaaggtataggctttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGCCACGCGGCTAAGTGAGTAACCTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTtcc                                                                                                                           <  1:163722‑M1/121‑2 (MQ=255)                                                                                                                            ttcacgcgtcggtttgcattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGcc                                                                                                                 <  1:268479‑M1/131‑1 (MQ=255)                                                                                                                             tcacgcgtcggtttgaattctttattgtcgtgccacttcacacctttacgcagatggaatgtataggttttaccgtctgcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCagag                                                                                                                                                                                               >  1:345350‑M1/120‑172 (MQ=255)                                                                                                                             tcacgcgtcggtttgaattatttattgtagtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCagag                                                                                                                                                                                               <  2:345350‑M1/53‑1 (MQ=255)                                                                                                                                       gtttgaattctttattgtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggctcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGc                                                                                                      <  1:142366‑M1/142‑1 (MQ=255)                                                                                                                                          tGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTg                                                                                                    >  2:273073/1‑250 (MQ=255)                                                                                                                                                       gtcgtgccacttcacacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGtt                                                                                      <  2:267018‑M1/158‑1 (MQ=255)                                                                                                                                                            gccacttcacacctttacgcagatggaatgtataggttttaccgtcttcgctgacttcccacttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGc                                                                                 >  1:57330‑M1/89‑251 (MQ=255)                                                                                                                                                                     cacctttacgcagatggaaggtataggttttaccgtcttcgctgacttcccactcttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCg                                                                        >  1:124114‑M1/80‑251 (MQ=255)                                                                                                                                                                                  ctggaaggtatcagttttaccgccttcgctgacttcccactcttgagcgaggcacgcgatcactgaTCTTACCCCGGAATAGCGGACACGCGGCTAAGTGAGTACACTCTCAGTCAGAGGTGACTCACATGACCAAAACAGTATCAACCAGTAAAAAACCCCGTCAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAACCTTGCTGAACGCATCGGCGTTACTGCCGCAGCCCGTGACCTCAGCCTg                                                           <  2:158423‑M1/185‑1 (MQ=255)                                                                                                                                                                                       aaGATAAGCGCCCGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATg                                                       >  2:170030/1‑250 (MQ=255)                                                                                                                                                                                         gATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAGCCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCATCCCGTGTACTCAGCCTGTATGAAt                                                    <  1:70621/251‑1 (MQ=255)                                                                                                                                                                                                    gAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTAc                                         >  2:70955/1‑251 (MQ=255)                                                                                                                                                                                                     ccgtcttcgctgacttccaccttttcagcgaggcccgggatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAACTGAGTAACCTCTCAGTCAGAGGTGACTCACATGACCAAAACAGTATCAACCAGTCAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGATGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTacc                                        <  2:317033‑M1/204‑2 (MQ=255)                                                                                                                                                                                                            cgctgacttcccacttttcagcgaggcccgagatcactgaTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCTGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGgc                                  >  1:871‑M1/41‑250 (MQ=255)                                                                                                                                                                                                                  cTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACCCGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTa                            >  2:40542/1‑250 (MQ=255)                                                                                                                                                                                                                   ttcccacttttcagcgaggcccgggatcactgaTCTCCACCAGCCATAGTGGATACGCGGCCAACTGAGTAAACTCTCCGTCAGAGGTGACTCACATGCCCAAAAAAGTATCAACCAGTAAACAACACCGTAAACAGCATTCGCATGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTAt                                                        <  1:322991‑M1/188‑1 (MQ=255)                                                                                                                                                                                                                    aTTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGCGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTatac                         >  2:105320/1‑248 (MQ=255)                                                                                                                                                                                                                             gAGGTGACCTATACGCCAGTGCCGCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTAGCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCAGCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCATTAAACAGCAAAATc                <  2:50544/251‑1 (MQ=255)                                                                                                                                                                                                                              aGGTGACCTATACGCAAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAACATc                >  1:76685/1‑250 (MQ=255)                                                                                                                                                                                                                                           gCCAGTGCCTCTTACCCAGCAATAGCGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGtcttct  >  1:101600/1‑251 (MQ=18)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  GAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTCGTGCTGAGGGCTATTTACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGATAAGCGCCAGAAAGACCCGCATGCTATTTCTGCCGAGGTGACCTATACGCCAGTGCCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTCT  >  NC_000913/314986‑315470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.