Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,016,437	Δ2 bp :: IS186 (–) +8 bp :: Δ1 bp	coding (570‑577/687 nt)	fliH →	flagellar biosynthesis protein FliH

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2016437 =	1 (0.020)	75 (1.460)	69/482	0.0	98.6%	coding (570/687 nt)	fliH	flagellar biosynthesis protein FliH
	?	NC_000913	2514274 =	NA (NA)					noncoding (2/1345 nt)	IS186	repeat region
*	?	NC_000913	= 2016444	2 (0.040)	61 (1.190)	59/484	0.1	96.7%	coding (577/687 nt)	fliH	flagellar biosynthesis protein FliH
	?	NC_000913	= 2515615	NA (NA)					noncoding (1343/1345 nt)	IS186	repeat region

AATTGACGACATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCAGC  >  NC_000913/2515374‑2515864                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                          aaTTGACGACATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTcc                                                                                                                                                                                                                                                  <  1:298486/251‑1 (MQ=32)          cATAATCCAGCCATCGCTGGATTTCCCCCCCATAAGTGCCTGATCCGAAAAGAAGAACTAACTCGTTGTGGATAATAACAAAAATGTTCATCTGGAGCTTACAGTTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTGCAAAACGCAATTGAATAGCGCTAGGCATCGTCGTAACGATGTACCGATGCGTCGCATTCTGCAG‑TGGTTTCTGCCCTTAAGTTATCGCTGATGGGATAATTCCCCGGttttt                                                                                                                                                                                                                                         <  1:65694/251‑1 (MQ=11)          cATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGACGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGttttt                                                                                                                                                                                                                                         <  1:209169/251‑1 (MQ=25)             aaTCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTa                                                                                                                                                                                                                                      <  2:111675/251‑1 (MQ=37)              aTCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTGGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATGGAAGAACGCGAGGCAGCGTCTTAACGAGGCACCGAGGCGTCGCTTTCTTAAGATGGTT‑CAACCCTTAAGTTAGCGCTTAGGGGTTAATTCCCCGTTTTTTTTAc                                                                                                                                                                                                                                     >  2:437216/1‑251 (MQ=11)                     cATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGtt                                                                                                                                                                                                                              >  2:70503/1‑251 (MQ=40)                       tCGCTGGATTTCCCCCCCAGAAGAGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTACCGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTa                                                                                                                                                                                                                             >  2:272388/1‑250 (MQ=255)                        cGCTGGAGTTCCCACCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAAt                                                                                                                                                                                                                           <  2:62987/251‑1 (MQ=255)                             gATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATGCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAAGCAGCt                                                                                                                                                                                                                      <  1:383862/251‑1 (MQ=255)                                    cccccAGAAGGGCCGGATCCGAAAAGAAGAACTAACTCTTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATACGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAGCGCGAGGCATCGTCTTAACGAGGCACCGATGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGGTTTTTTACGCCTGGTAATCAGCTAATGGCt                                                                                                                                                                                                               <  2:348511/251‑1 (MQ=14)                                          gAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGc                                                                                                                                                                                                         <  1:82708/251‑1 (MQ=255)                                                    aTCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGTATAATTCCCCGGTTTGTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCaa                                                                                                                                                                                               <  2:465967/251‑1 (MQ=255)                                                         aaaaGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATAcc                                                                                                                                                                                          <  2:151285/251‑1 (MQ=255)                                                               agaaCTAACTCGTTGTGGAGAATAACAACAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGCCCAATCAAATACCCTTGc                                                                                                                                                                                     >  2:401860/1‑250 (MQ=255)                                                                         cGTTTTGGAGAATAACAAAAAAGGTCATCTGGAGCTGACAGGTGGCCATCCTTGGGACAGTATCCCGGACA‑GCCTACAAAACTCAATTGAAGAACTCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATTGTT‑CAACCCTTAAGTTAGCTCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCTGGCGACCAATCAAATACCCTTGCAGTTATTGCAc                                                                                                                                                                          <  2:1566/251‑1 (MQ=18)                                                                         cGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCAc                                                                                                                                                                          >  1:14550/1‑251 (MQ=255)                                                                             gtgGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCg                                                                                                                                                                      >  1:133153/1‑251 (MQ=255)                                                                                            aaTGGTCATCTGGAGCTTACAGTTGGCCATTCGTGGGACTGTATCCCTG‑CACGCCTACAAAACGCAATTGACGAACGCGAGGCATCGGCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CCACCCTTAAGTTCGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCACGAGCTTCTGCAATAgc                                                                                                                                                       >  2:338831/1‑251 (MQ=21)                                                                                            aaTGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAgc                                                                                                                                                       >  2:416940/1‑251 (MQ=255)                                                                                                  cATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCctgc                                                                                                                                                 <  2:301684/251‑1 (MQ=255)                                                                                                               cAGGTGGCCAGTCGTGGGACAGTATCCCTGACA‑GCCTACCAAACGCAATTGAAGAACTCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATGCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATAGTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGtct                                                                                                                                    <  2:124898/251‑1 (MQ=255)                                                                                                                aGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGtctc                                                                                                                                   >  1:195814/1‑251 (MQ=255)                                                                                                                aGGTGACCATTCGTGGGCCACTATCAATGCCA‑CCCTCCAAAACGCAATTGCAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTAGCATCCTTCAGATGGTT‑CAACCCTTACGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACAACTCAAATACCCTTGCAGATATTGCACCACGAGCTTATGCAATAGCGCCTGCTGCTGTTGCggtc                                                                                                                                    >  2:253339/1‑247 (MQ=25)                                                                                                                                  cAGTATCACTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGCCTGCGGGCGACCAACCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACcac                                                                                                                 >  1:155207/1‑251 (MQ=255)                                                                                                                                             aCA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCTCATTCTGCAGATGGTT‑CAACCCTTAAGTTAGCGCGTATGGGATAAGTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGGGGGCGACCa                                                                                                                                                                                                    <  2:314953/157‑1 (MQ=18)                                                                                                                                             aCA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCa                                                                                                                                                                                                    >  1:314953/1‑157 (MQ=255)                                                                                                                                               a‑GCCGACAAAACGCAAGTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCTCCTGCTGCTGTTGCGTCTCGACAAACGCCGCTACCACACTCAATGACTTc                                                                                                    <  2:195814/251‑1 (MQ=255)                                                                                                                                                     aCAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCt                                                                                                 <  2:387260/249‑1 (MQ=255)                                                                                                                                                          aCGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCAcc                                                                                                                                                                         >  1:168618/1‑172 (MQ=255)                                                                                                                                                          aCGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATGGCAcc                                                                                                                                                                         <  2:168618/172‑1 (MQ=255)                                                                                                                                                           cGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCTCCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAg                                                                                         <  1:299845/251‑1 (MQ=255)                                                                                                                                                                  gaagaaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCCATGACTTCGCTTTCGCCAGATCGGt                                                                                   >  2:187546/1‑250 (MQ=255)                                                                                                                                                                     gaaCGCGAGGCATCGTCTTAACGAGGCACCGTGGCGTCGCATTCTTCAGATGGTT‑CAACACTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCACATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGTCAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCCGATCGGTAAtt                                                                               >  2:365931/1‑251 (MQ=255)                                                                                                                                                                                       tAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGc                                                              <  2:133153/250‑1 (MQ=255)                                                                                                                                                                                           gAGGCACAGAGGCGTCGCATTCTTCAGATGGCT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATccc                                                         >  2:201209/1‑250 (MQ=255)                                                                                                                                                                                                     ggCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGt                                               <  1:338831/251‑1 (MQ=255)                                                                                                                                                                                                          cGCATTCTTCAGATGTTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGTCGGCGGGCGACCAATCAAATACCCTTGCAGATATTGCAcccc                                                                                                                                                                       <  1:471034/126‑1 (MQ=255)                                                                                                                                                                                                          cGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAACTCCCCGGTGTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTTCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCTGTAATTGATCGCTCAAGCCTCGCATCCAGCGTGTTCGTGACa                                           <  2:474220/250‑1 (MQ=255)                                                                                                                                                                                                          cGCATTCCTCAGATGGTT‑CAACCCTTAAGTTAGCGATTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTCATCAGCTACTGGCTGCGGGCGACCAATCAAATACCCTTGCAGAAATTGCAcccc                                                                                                                                                                       >  2:471034/1‑126 (MQ=255)                                                                                                                                                                                                                          tt‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGAc                          >  1:393426/1‑251 (MQ=255)                                                                                                                                                                                                                          tt‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACAACTCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGAc                          >  1:484230/1‑251 (MQ=255)                                                                                                                                                                                                                              aaCCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGGTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGcc                       <  2:14550/251‑1 (MQ=255)                                                                                                                                                                                                                                cccTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTcac                                >  1:313440/1‑240 (MQ=255)                                                                                                                                                                                                                                cccTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCAt                     <  1:201209/251‑1 (MQ=255)                                                                                                                                                                                                                                cccTTAAGTTAGCGCTGATGGGATAATTCCCCGGTTGTTTGACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCGCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCTCAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTcac                                <  2:313440/240‑1 (MQ=255)                                                                                                                                                                                                                                     aaGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTCCGACTGTTAATTCGCTAATGGCTGAGGGAGACCACTCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGAGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATAATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAATACGCCATCAAtt                >  1:325190/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAAt                 <  2:393426/250‑1 (MQ=255)                                                                                                                                                                                                                                      aGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAAttt               >  2:401753/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAAtttt              <  1:416940/251‑1 (MQ=255)                                                                                                                                                                                                                                                tATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGatat      <  1:187546/250‑1 (MQ=255)                                                                                                                                                                                                                                                   gggATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCAGc  >  1:534925/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                          AATTGACGACATAATCCAGCCATCGCTGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACA‑GCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAATGACTTCGCTTTCGCCAGATCGGTAATTGATCTCACAATCATCGCATCCAGCGTGTTCGTGACAATATCTTTCACAAAGACGCCATCAATTTTGATGATATCAGC  >  NC_000913/2515374‑2515864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.