Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,090,238	688 bp→GGGGATCTTGAAGTTCCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCAGAGCGCTTTTGAAGCT		[yggI]–[endA]	[yggI], [endA]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3090238	3090925	688	73 [0]	[0] 78	[yggI]–[endA]	[yggI],[endA]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3090237	0 (0.000)	56 (1.410) +GGGGATCTTGAAGTTCCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCAGAGCGCTTTTGAAGCT	50/334	0.1	100%	coding (483/498 nt)	yggI	protein YggI
	?	NC_000913	3090926 =	0 (0.000)					coding (580/708 nt)	endA	DNA‑specific endonuclease I

GAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTTCTCAGGCGAAAGCCGCGGCGGTAAAAGTCCACGCTGACGCGCCCGGTACGT  >  NC_000913/3089989‑3090482                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      gAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAACGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGgg                                                                                                                                                                                                                                                     >  1:594473‑M1/1‑249 (MQ=255)      ggCACATTTGCTGGTATGGAAACATTTCGGCCGCTTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCTTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggat                                                                                                                                                                                                                                                 <  1:211761‑M1/250‑7 (MQ=255)                      tGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattcc                                                                                                                                                                                                                                >  1:39351‑M1/1‑228 (MQ=255)                       ggAAACATTTCGGCCGCGTAGCGCCCCATGGCAAAGAGGGGAAGTGGATGAGGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCTCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccg                                                                                                                                                                                                                               <  2:169994‑M1/251‑25 (MQ=255)                          aaCAGTTCGGCCGCGTGGCGCCGCAGGGAAAAGAGTGGAAGTGGATGAGGGCAAACGGGCTGGGTGTGCCCGCCCGTCGTCCGCATCAGTTCTACCTGCAAGCCGTGCGTCGCAACACCTTCCCTTACCGCTGCAATTGCCAGGAGCATAATCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggtgatcttgaagttcctattccgaag                                                                                                                                                                                                                            <  2:518010‑M1/251‑28 (MQ=255)                            cATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGAATGATGGAAAACGTGCTGGGTGTTCCCGACCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGAAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagtt                                                                                                                                                                                                                          >  1:534056‑M1/1‑222 (MQ=255)                                tCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcct                                                                                                                                                                                                                       <  1:296912‑M1/250‑33 (MQ=255)                                    cagcgtaGCGCCACATGGCAGAGATTGGAAGTGGATGAGGGAAAACGTGCTGGGTGTCCCCGCCCGTCGTACGCATAGGTTCGAACTGCAATCCGTTCGTCGAAACCCCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTTCG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattct                                                                                                                                                                                                                  <  2:182460‑M1/249‑38 (MQ=255)                                     cgcgTAGCGCCACATGGCAAAGCGTGGAAGTGGATGCTGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagatcctattctc                                                                                                                                                                                                                 >  2:58667‑M1/1‑213 (MQ=255)                                     cgcgTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctc                                                                                                                                                                                                                 >  1:341308‑M1/1‑213 (MQ=255)                                       cgTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGCCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctcta                                                                                                                                                                                                               <  1:139105‑M1/251‑41 (MQ=255)                                                acaTGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtat                                                                                                                                                                                                       >  2:78884‑M1/1‑202 (MQ=255)                                                acaTGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaa                                                                                                                                                                                                            >  2:435407‑M1/1‑202 (MQ=255)                                                acaTGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaa                                                                                                                                                                                                            <  1:435407‑M1/245‑44 (MQ=255)                                                              ggaagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcg                                                                                                                                                                                        >  1:348884‑M1/1‑188 (MQ=255)                                                                aagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctatt                                                                                                                                                                                                                    >  2:511461‑M1/1‑186 (MQ=255)                                                                aagtggaTGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctatt                                                                                                                                                                                                                    <  1:511461‑M1/221‑36 (MQ=255)                                                                           ggAAACCGTGCTGGGGTTTCCCGCCCGTCTTACGCAGCTGTTCGAACGGCAATCGGTGCGTCGCAACGCCTCTCCCTTCCGCTGCAAGTGCCAGGAGTATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgtttttgaagctct                                                                                                                                                                           <  2:301125‑M1/251‑77 (MQ=255)                                                                                      tGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcat                                                                                                                                                                >  1:215506‑M1/1‑164 (MQ=255)                                                                                       gggTGTCCCCTCCCTTCGTACGCACAAGGTCAAACTGCAATCCGTGCGTCGCACCCCCTTCCCCTCCCGCTGCAAGGGCCAGGAGCATCAGCTTCCCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgca                                                                                                                                                                 <  2:348884‑M1/249‑87 (MQ=255)                                                                                             tCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCATCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggctcttgaagttcctattccgaagttcctattctctcgaatgtataggaacttcagagcgcttttgaagctctgttcaacgcatggaaca                                                                                                                                                          >  2:292245‑M1/1‑157 (MQ=255)                                                                                              cccgcccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaaca                                                                                                                                                          >  1:595763‑M1/1‑156 (MQ=255)                                                                                                  cccgTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgt                                                                                                                                                    <  1:2234‑M1/251‑100 (MQ=255)                                                                                                       cgtACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaactcatggaacaagatgtatccg                                                                                                                                               >  2:489674‑M1/1‑147 (MQ=255)                                                                                                        gtACGCATCAGTTCGAACTGCAATCCGAGCGTCGCAACACCTTCCCCTACCGCTGCCCGTGCCCGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGGGAACAGggggatcttgaagttcgtattccgaatgtcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccgg                                                                                                                                              >  2:35017‑M1/1‑146 (MQ=255)                                                                                                          aCGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACCCCTTCCCCTACCGCTGCAAGGGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggtt                                                                                                                                            <  1:459553‑M1/251‑108 (MQ=255)                                                                                                          aCGCATCAGTGCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggtt                                                                                                                                            <  1:416513‑M1/251‑108 (MQ=255)                                                                                                             cATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATAGCGTAGATCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgacgtccctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttacc                                                                                                                                         >  1:345592‑M1/1‑141 (MQ=255)                                                                                                                   ttCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgg                                                                                                                                   >  1:199439‑M1/1‑135 (MQ=255)                                                                                                                     cGAACTTCAATCCTGGCGTCGCAACCCCTTCCCCTCCCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactggg                                                                                                                                  <  2:444192‑M1/250‑118 (MQ=255)                                                                                                                        aCTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtg                                                                                                                              <  1:461288‑M1/251‑122 (MQ=255)                                                                                                                         cTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgc                                                                                                                             >  1:382878‑M1/1‑129 (MQ=255)                                                                                                                           gcactccgtGCGCCGCAACACCCTCCCCTACCGCTGCAAGTGCCAGGGGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGGGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaaggataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcga                                                                                                                           <  2:357749‑M1/247‑125 (MQ=255)                                                                                                                             aaTCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagc                                                                                                                         >  2:477‑M1/1‑125 (MQ=255)                                                                                                                                ccGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcg                                                                                                                      >  1:311619‑M1/1‑122 (MQ=255)                                                                                                                                  gTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgat                                                                                                                    >  1:472581‑M1/1‑120 (MQ=255)                                                                                                                                   tGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACCGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttca                                                                                                                                                                      >  1:258843‑M1/1‑119 (MQ=255)                                                                                                                                               ccTTCCCCTCCCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATCATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGCACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtctaggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgcgcgcgatgaacgcatcgcg                                                                                                        >  2:548796‑M1/1‑107 (MQ=255)                                                                                                                                                cTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggcgtgcgagcgcgatgaacgcatcgcga                                                                                                       >  2:360082‑M1/1‑106 (MQ=255)                                                                                                                                                 ttCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGCGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcatattctctagaaagtatcggaacttcagagcgcttttgaagctatgttcaacgcatggaacaagatgtatccggttaacgactgggagtgcgaacgcgatgcacgcatcgcga                                                                                                       >  1:553485‑M1/1‑105 (MQ=255)                                                                                                                                                  tCCCCTACCGCTGAAAGTGCCAGGGGCATTAGCGTACCGTACT‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTAGCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagtttctattctctagaaagtataggaacttcagagcgcttttgaagctctggtcaacgcatggaacaagatgtatccggttaccgacgtggagtgcgagcgcgatgaacgcatcgcgaag                                                                                                     <  1:584998‑M1/250‑147 (MQ=255)                                                                                                                                                       cccctcgtcaagtgccgGGGGCATCA‑CTTTCCGTTCGCCCGCCATAATCGCGTATTTCGTGGCGAGGCCGTCTATCGCTGTGTGCACTGCGGTGAACAGggggatcttgaagttcctattcctaagttcctattctctagaaagtataggaactgcagagcgcttttgaagctctgttcaacgcatggaacaagatgaatccggttaacgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcag                                                                                               <  2:343656‑M1/243‑153 (MQ=255)                                                                                                                                                                 agggccaGGAGCATCAGCTTACCGTCCG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGGGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgctaaggtgcagggcaatcata                                                                                     <  2:594473‑M1/248‑163 (MQ=255)                                                                                                                                                                   tGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataac                                                                                   >  1:377749‑M1/1‑87 (MQ=255)                                                                                                                                                                      cAGGAGCAGCAGCTTACCTGACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccg                                                                                <  2:335277‑M1/251‑168 (MQ=255)                                                                                                                                                                             aTCAGCTTACCGTACG‑CCGCCATAATCGAGAAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCAATGCGGTGAACAGggggatcttgaagttcctattccgaagttcctcttctctagacagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacacg                                                                                                                                                        >  2:357983‑M1/1‑77 (MQ=255)                                                                                                                                                                             aTCAGCTGACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaag                                                                                                                                                        <  1:357983‑M1/172‑96 (MQ=255)                                                                                                                                                                                aGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaac                                                                      <  2:199439‑M1/251‑178 (MQ=255)                                                                                                                                                                                 gCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacg                                                                     <  2:447515‑M1/251‑179 (MQ=255)                                                                                                                                                                                   ttACCGCACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcg                                                                   >  1:161303‑M1/1‑71 (MQ=255)                                                                                                                                                                                     cccgTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccg                                                                                <  2:388552‑M1/235‑168 (MQ=255)                                                                                                                                                                                     aCCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccg                                                                                >  1:388552‑M1/1‑69 (MQ=255)                                                                                                                                                                                          aCG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgcc                                                             >  1:406135‑M1/1‑64 (MQ=255)                                                                                                                                                                                              ccgccATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggc                                                         <  1:369420‑M1/251‑191 (MQ=255)                                                                                                                                                                                              ccgccATAAGCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatctctaaggtgcagggcaatcataacccttatgtgcaacgcgcttgccaggc                                                         <  2:297016‑M1/251‑191 (MQ=255)                                                                                                                                                                                                  cATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcg                                                                                                        >  2:267479‑M1/1‑57 (MQ=255)                                                                                                                                                                                                  cATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcg                                                                                                        <  1:267479‑M1/200‑144 (MQ=255)                                                                                                                                                                                                           gTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaa                                            <  2:375555‑M1/251‑204 (MQ=255)                                                                                                                                                                                                           gTAGTTCGGGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaa                                            <  2:311619‑M1/251‑204 (MQ=255)                                                                                                                                                                                                             aGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaac                                           <  2:341308‑M1/250‑205 (MQ=255)                                                                                                                                                                                                                 cGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCCGTGAACAGggggatcttgaagttcctattccgaagatcctattctctagaaagtataggaaattcagagcgcttttgcagctctgttcaacgcctggaccaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgcaaggcgcgaaagagataacctac                                       >  1:343018‑M1/1‑42 (MQ=255)                                                                                                                                                                                                                      cGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagc                                 <  1:78884‑M1/251‑215 (MQ=255)                                                                                                                                                                                                                         ggCCGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcggg                              >  1:325545‑M1/1‑34 (MQ=255)                                                                                                                                                                                                                           ccGTCTATCGCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgcgaggcgcgaaagagctaacctacactagcgggat                            <  1:548796‑M1/251‑220 (MQ=255)                                                                                                                                                                                                                                tATCGCCGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcgggattcttt                       >  1:154682‑M1/1‑27 (MQ=255)                                                                                                                                                                                                                                    gCTGTGTTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcgggattctttttgt                   <  1:58667‑M1/251‑229 (MQ=255)                                                                                                                                                                                                                                       gtgtTCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcgggattctttttgtta                 <  2:39351‑M1/250‑231 (MQ=255)                                                                                                                                                                                                                                       gtgtTCACAGCGGTGAACAGggggatcttgaagttcctatgccgaaggtcctattctctagaaagtataggaacttcagagcgctgttgaagcgctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcatggcaatcataacccgtatgtgcaacgcgtttgccaggcgcgaaatagctaacctacactagcgtgattctttttgtta                 <  1:476589‑M1/250‑231 (MQ=255)                                                                                                                                                                                                                                           tCACTGCGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcgggattctttttgttaaccc             <  2:382878‑M1/250‑235 (MQ=255)                                                                                                                                                                                                                                                 cGGTGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcgggattctgtttgttaacccctacccc      <  2:215506‑M1/251‑242 (MQ=255)                                                                                                                                                                                                                                                    tGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcgggattctttttgttaacccctaccccacg   >  2:436789‑M1/1‑7 (MQ=255)                                                                                                                                                                                                                                                    tGAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcgggattctttttgttaacccctaccccacg   >  1:166664‑M1/1‑7 (MQ=255)                                                                                                                                                                                                                                                     gAACAGggggatcttgaagttcctattccgaagttcctattctctagaaagtataggaacttcagagcgcttttgaagctctgttcaacgcatggaacaagatgtatccggttaccgactgggagtgcgagcgcgatgaacgcatcgcgaaggtgcagggcaatcataacccgtatgtgcaacgcgcttgccaggcgcgaaagagctaacctacactagcgggattctttttgttaacccctaccccacgc  >  1:306984‑M1/1‑6 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      GAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGCTGGGTGTTCCCGCCCGTCGTACGCATCAGTTCGAACTGCAATCCGTGCGTCGCAACACCTTCCCCTACCGCTGCAAGTGCCAGGAGCATCAGCTTACCGTACG‑CCGCCATAATCGCGTAGTTCGTGGCGAGGCCGTCTATCGCTGTGTTCACTGCGGTGAACAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGTTTTAACACGGAGTAAGTGATGTACCGTTATTTGTCTATTGCTGCGGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAGTTTTTCTCAGGCGAAAGCCGCGGCGGTAAAAGTCCACGCTGACGCGCCCGGTACGT  >  NC_000913/3089989‑3090482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑