Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,262,279	Δ6,678 bp		[fruK]–[lpxT]	[fruK], fruB, setB, yeiW, yeiP, yeiQ, yeiR, [lpxT]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2262279	2268956	6678	20 [0]	[0] 18	[fruK]–[lpxT]	[fruK],fruB,setB,yeiW,yeiP,yeiQ,yeiR,[lpxT]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2262278	0 (0.000)	18 (0.500)	17/484	1.0	100%	coding (88/939 nt)	fruK	1‑phosphofructokinase
	?	NC_000913	2268957 =	0 (0.000)					coding (104/714 nt)	lpxT	lipid A‑core phosphotransferase

CGTTGCGCCACCGGACAGCCGTATTGAGCTGATTTCCAGCAGCGAAGCTGACTGGAATGCCTTGCAGAGCGCGCTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTGGGTCAGGCAT  >  NC_000913/2268710‑2269187                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        caaaacccgagaagttgaagtcggtcacttcgccgtctttttccgtcagcttaactttaattcgggtgcccccctgtacaacctggaaccggttggcaatgcccagctcgctgaacagttgctgaaacccatcctgattgtctttacccaggaagccgccaacggtgacatcaattcccaggtcttttaatactttggccacgttgatgcctttccccgccgcatgcagaccggtggttttcaccagATg                                                                                                                                                                                                                                       <  1:329992‑M2/3‑1 (MQ=255)         gagaagttgaagtcggtcacttcgccgtctttttccgtcagcttaacgttaattcgggtgcgcccctgtacaacctggaaacggttggcaatgcccagctcgctgaacagttgctgaaaaccatcctgattgtctttacccaggaagccgccaacggtgacatcaattcccaggtcttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggttttcaccagATGCGGATAttt                                                                                                                                                                                                                              >  2:315890‑M2/240‑251 (MQ=255)                                                  cttaacgttaattcgggtgcgcccctgtacaacctggaaacggttggcaatgcccagctcgctgaacagttgctgaaaaccatcctgattgtctttacccaggaagccgccaacggtgacatcaattcccaggtcttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCttt                                                                                                                                                                                    >  2:27156‑M2/199‑251 (MQ=255)                                                                      gcccctgtacaacctgtaaacggttggcaataacccactcgctgaacagttgctgaaaaccatcctgattgtctttacccaggaagccaccaaaggggacatcaattcccaggtcttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGAc                                                                                                                                                                >  1:180292‑M2/179‑251 (MQ=255)                                                                       cccctgtacaacctggaaacggttggcaatgcccagctcgctgaacagttgctgaaaaccatcctgattgtctttacccaggaagccgccaacggtgacatcaattcccaggtcttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAAACAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTTAcc                                                                                                                                                               >  2:271149‑M2/178‑251 (MQ=255)                                                                         cctgtacaacctggaaacggttggcaatgccaagctcgctgaacagttactgaaacccatcctgattgtctttacccaggaagccgccaacggtgacatcaattcccaggtcttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGCCca                                                                                                                                                              >  1:234846‑M2/176‑250 (MQ=255)                                                                                      gaaacggttggcaatgcccagctcgctgaacagttgctgaaaaccatcctgattgtctttacccaggaagccgccaacggtgacatcaattcccaggtcttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTTTTTCTTT‑ATCAAGAACTTGTCGGAAGGAAAGGCTTTTTTTGGGTTGGTTGCATTTCCCAACAATCGCCCCTTc                                                                                                                                                >  2:143832‑M2/163‑251 (MQ=255)                                                                                                                                  catcctgattgtctttacccaggaagccgccaacggtgacatcaattcccaggtcttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGttt                                                                                                    >  1:205209‑M2/119‑251 (MQ=255)                                                                                                                                                       ggaagccgccaacggtgacatcaattcccaggtcttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGcccc                                                                               >  1:262836‑M2/98‑251 (MQ=255)                                                                                                                                                                                   caggacttttaatactttggccacgttgatgcctttacccgccgcatgcagaccggtggatttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTAttggg                                                   >  1:223087‑M2/70‑250 (MQ=255)                                                                                                                                                                                    gggtcttttaatactttggccacgttgatgcttttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtc                                                  <  1:27156‑M2/183‑1 (MQ=255)                                                                                                                                                                                                      gtcaggttgatgcttttacccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACGGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTg                                <  2:263125‑M2/201‑1 (MQ=255)                                                                                                                                                                                                                         ccgccgcatgcagaccggtgtttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGTGC‑GAAAGTAAGTCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCt             <  1:195341‑M2/220‑1 (MQ=255)                                                                                                                                                                                                                         ccgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCt             <  1:286999‑M2/220‑1 (MQ=255)                                                                                                                                                                                                                          cgccgcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTg            <  1:339784‑M2/221‑1 (MQ=255)                                                                                                                                                                                                                              gcatgcagaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTGGGTc        <  2:328133‑M2/225‑1 (MQ=255)                                                                                                                                                                                                                                  gccaaccggtgggtttcaccagGTGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTAGCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTGGGTCAGGc    <  1:143832‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                                    agaccggtggttttcaccagATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTGGGTCAGGCAt  >  1:308545‑M2/21‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        CGTTGCGCCACCGGACAGCCGTATTGAGCTGATTTCCAGCAGCGAAGCTGACTGGAATGCCTTGCAGAGCGCGCTGTTGAAGCTTCGTTTAGCGACTACCGCGTAAGGTTGCCTGCGTTTTTCAGTAAGATAATTAGAGAAAATATGATTAAAAATTTGCCGCAAATAGTGTTGTTGAATATTGTCGGCCTCGCGCTGTTTCTTTCCTGGTATATCCCCGTTAATCATGGATTCTGGTTGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTC‑GAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAAACCAGCTGGGTCAGGCAT  >  NC_000913/2268710‑2269187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑