Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,187,650	IS1 (–) +9 bp	coding (757‑765/1035 nt)	yehA ←	putative fimbrial adhesin YehA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	22 (0.770)	21/484	0.4	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 2187658	0 (0.000)					coding (757/1035 nt)	yehA	putative fimbrial adhesin YehA
*	?	NC_000913	= 1979270	NA (NA)	20 (0.700)	20/484	0.4	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	2187650 =	0 (0.000)					coding (765/1035 nt)	yehA	putative fimbrial adhesin YehA

TCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACC‑TCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCC  >  NC_000913/1979021‑1979271                                                                                                                                                                                                                                                           |  tcgaaCGCCTTTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGGAGCGGGGTAAGTGAACTGAAGTTGCCATGTTTTATGGCGGAGAGAGCAGAGATAGCGCTGGTGTCCGACGGTGCGTTTGCCGGTACGCACCACCCCGGCAGTATGTGAACCGGAGGGGAAGCTGTTAGACAAACAAGCCACTGGAGCACCCTCAAAC‑CACCTACAAACACCAAATCAGGAAGTTAGCAGGATCAccc  >  2:269424/4‑251 (MQ=2)                                                                                                                                                                                                                                                           |  TCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACC‑TCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCC  >  NC_000913/1979021‑1979271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.