Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	59,092	(15-bp)2→1	coding (619‑633/651 nt)	yabP →	putative uncharacterized protein YabP

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	59090 =	1 (0.030)	13 (0.490)	13/450	0.7	93.1%	coding (617/651 nt)	yabP	putative uncharacterized protein YabP
	?	NC_000913	= 59074	1 (0.040)					coding (601/651 nt)	yabP	putative uncharacterized protein YabP

ATCCCACGCTACTGCTAAGGTTGAAGGGGCGTTCCAGCCCCTTCAACCTTAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/59332‑59090 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggttagcaccgtccgggGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGGCCCTTATGCAGCTGCAACGTCTGAGT  <  NC_000913/59074‑58888                                                                                                                                                                                                                                                                                                                                                                                                                                                ATCCCACGCTACTGCTATTGTTGAATGGGCGTTCCAGCCCCTTCAATCTTAGCTTATTCTGGCTCATTCAATAATTTCGTTGTCATTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCATTTTTTTTGGGGGGTATTTTATTAATAATAATTGCCTCATCATATAAGGATGTAGCACCGTTCACGGTAGCGCCGTCCAGGTTAGCACCGTCCGGTGTAGCACC                                                                                                                                                                                     <  2:194014/251‑1                                      CCCCTTCAACCTTAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTCTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAG                                                                                                                                                <  1:221011/251‑1                                       CCCTTCAACCTTAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCAC                                                                                                                                                    <  1:245521/246‑1                                       CCCTTCAACCTTAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCAC                                                                                                                                                    >  2:245521/1‑246                                                  TAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATga                                                                                                                                     >  1:135648/1‑248                                                                   TTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCGACTCCTTTAGTATCAATATTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCCGGTTAGCATTCCACCCAGGCCTCTCATAAAGGATATCATTGCTACCT                                                                                                                   >  2:174442/1‑251                                                                         AATTGCGTTGTCAGTAATTTGTTCAGCAGTAATTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACAGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGAATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATT                                                                                                             >  1:313041/1‑251                                                                                                              TAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGCTTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGC                                                                        >  1:148266/1‑251                                                                                                                                   CAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTA                                                    >  2:167499/1‑250                                                                                                                                        CCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCA                                              <  1:147201/251‑1                                                                                                                                                                                     ACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGGCCCTTATGCAGCTGCAACGTCTGAGT  <  1:167499/250‑1                                                                                                                                                                                                TAAGGAGGTATCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGGCCC                         >  2:69433/1‑216                                                                                                                                                                                                TAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGGGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGGCCC                         <  1:69433/216‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                ATCCCACGCTACTGCTAAGGTTGAAGGGGCGTTCCAGCCCCTTCAACCTTAGCTTATTCTGGCTCATTCAATAATTGCGTTGTCAGTAATTTGTTCAGCAGTACTTTTTTAGTAGCAACTTCTTCAGTAGCAACTCCTTTAGTATCAATTTTTTTGGGGGGGATTTTATTAATAATAATTACCTCATCATATAAGGAGGTAGCACCGTTCACGGTAGCACCGTCCAGGTTAGCACCGTCCGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/59332‑59090 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggttagcaccgtccgggGTAGCACCGTCCGGGGTAGCACGCCCAGGTTAGCATTCCACCCAGGTCTCTCATAAAGGATATCATTGCTACCTTTCATTGTTTTTTTACCAAGGTAACAATCCCTCATGTCAACGCCGGGAAGGTAAGCCTTTCTAAGATCAACGCCCAAGTTACCTTGGCCCTTATGCAGCTGCAACGTCTGAGT  <  NC_000913/59074‑58888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.