Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,909,925	IS5 (+) +4 bp	coding (172‑175/792 nt)	kdgR ←	DNA‑binding transcriptional repressor KdgR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1909925 =	0 (0.000)	25 (0.500)	23/482	0.8	100%	coding (175/792 nt)	kdgR	DNA‑binding transcriptional repressor KdgR
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1909928	0 (0.000)	24 (0.480)	23/480	0.8	100%	coding (172/792 nt)	kdgR	DNA‑binding transcriptional repressor KdgR
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

ACGACGCCCAATCCGTGAATACATGCGCAAATTGTACATAGAGTCAATTTTGTGAATGTAAACAATACTGTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAgg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1909683‑1909928 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGA  <  NC_000913/2290113‑2289873                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         ACGACGCCCAATCCGTGAATACATGCGCAAATTGTACATAGAGTCAATTTTGTGAATGTAAACAATACTGTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAA                                                                                                                                                                                                                                               <  1:172940/250‑1     CGCCCAATCCGTGAATACATGCGCAAATTGTACATAGAGTCAATTTTGTGAATGTAAACAATACTGTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGC                                                                                                                                                                                                                                          <  1:7301/251‑1              CGTGAATACATGCGCAAATTGTACATAGAGTCAATTTTGTGAATGTAAACAATACTGTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGGATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGACAGTGCGAACAAGTC                                                                                                                                                                                                                                 >  1:96718/1‑251                         GCGCAAATTGTACATAGAGTCAATTTTGTGAATGTAAACAATACTGTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAG                                                                                                                                                                                                                      <  1:69805/251‑1                                  GTACATAGAGTCAATTTTGTGAATGTAAACAATACTGTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTT                                                                                                                                                                                                             <  1:102659/251‑1                                       aAGAGTCAATTTTGTGAATGTAAACAATACTTTCTTCGTCCAGTGCGACGAGGTGGATAGTGTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATGAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCAT                                                                                                                                                                                                        <  2:97206/250‑1                                              AATTTTGTGAATGTAAACAATACTGTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGC                                                                                                                                                                                                 >  1:332594/1‑251                                                             atcaatccagaCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTTGTCATGCGGGAGAGCTCACGCATCTTGATATCTTCTCTCCTAATTAATTCGGCGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAAGTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATTACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCTTGTTTGTCATCTGGCGCCATAGAACAGGGTTC                                                                                                                                                                                  <  2:437424/240‑1                                                               CAATACTGTCTTCGTCCAGTGCGACGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGCTATCTGCGCTACGAATTACATCGACGTTTTGTAACGCGCGAGCGCCCCGTTCAAACAATTTCAGGGTAAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTACGGAAGGTGCGAACAAGTCTCTGATATTCGGTCATGTTTGTCATCTGGCGCCAAAAAACAGGTTTCA                                                                                                                                                                                 >  2:560168/1‑250                                                                      ggCTTCGGCCATTGCGCCGATGTGGATAGTTTCTTTGGGCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTTCGAATTAAATCGACGTTTTGTAACGCGCGATCGCCCAGTTCAAACAATTTCAGGGTGAGGGAATATTTCTCCGACTCCCCTTCCGGCGCCACTTATCCTAAGGAATGGGCTAACAAGTCCCTGATATTAGATCATGTTTGTCATCTGGAGCCCTAGAACAGTGTTCATCATG                                                                                                                                                                            <  2:481134/246‑1                                                                      GTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGA                                                                                                                                                                           <  1:312047/249‑1                                                                      GTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGA                                                                                                                                                                           <  1:319339/249‑1                                                                                         AGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCG                                                                                                                                                      >  1:68200/1‑251                                                                                                  GTTTCTTTGGGCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAAT                                                                                                                                             <  1:539020/251‑1                                                                                                        TTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAG                                                                                                                                         <  1:236273/249‑1                                                                                                                      GAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAG                                                                                                                         <  1:161496/251‑1                                                                                                                                                aaatAATTAAATCGACGTGTTGTAACTCGCGAGCGCACAGTTCAAACAATGTCAGGGGCAGGGAATATTTATCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCGGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTGCGTCGACAGTGAATTCAGCAGTAAGCGCCGTCAGTCCAGAAAAGAGATTTTCTTTTCCCG                                                                                               <  2:138903/247‑1                                                                                                                                                               CGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCT                                                                                <  1:543143/251‑1                                                                                                                                                                GTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTG                                                                               <  1:371826/251‑1                                                                                                                                                                     GTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGTAATATTTCTCAGACTCCCCTGCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCAGGTTTGTCATCTGGAGCTATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAAGTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATGTTCTTGTCCCGCATGGAGCAGATTCTGCCATG                                                                          <  2:11777/251‑1                                                                                                                                                                                   CCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGG                                                            >  2:238380/1‑251                                                                                                                                                                                     CAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGCGCAGATTCTGCCATTGCAAAACATGGTGGAA                                                          <  1:131533/251‑1                                                                                                                                                                                          CAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCAT                                                     <  1:238380/251‑1                                                                                                                                                                                                                                              ATAACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGAAATGGCCGGCGCCCTTATCCGCTGGA  >  2:271199/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         ACGACGCCCAATCCGTGAATACATGCGCAAATTGTACATAGAGTCAATTTTGTGAATGTAAACAATACTGTCTTCGTCCAGTGCGCCGAGGTGGATAGTTTCTTTGGTCAGGCGGGAGAGCTCACGCATCTGGATATCTGCGCTACGAATTAAATCGACGTTTTGTAACGCGCGAGCGCCCAGTTCAAACAATTTCAGGGTCAGGGAATATTTCTCCGACTCCCCTTCCTGCGCCACATAACCTAAgg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1909683‑1909928 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGA  <  NC_000913/2290113‑2289873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.