Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	20,703	+GCAAGAACCTGCAA	intergenic (‑195/+112)	insA1 ← / ← rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 20703	1 (0.020)	24 (0.420) +GCAAGAACCTGCAA	18/454	1.6	96.2%	intergenic (‑195/+112)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20
	?	NC_000913	20704 =	1 (0.020)					intergenic (‑196/+111)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

AATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAA‑CCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGC  >  NC_000913/20540‑20943                                                                                                                                                                    |                                                                                                                                                                                                                                                  aaaatagagtggtcttaagtttaatacctcaatgtgtatcacaatatccacatTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgctt                                                                                                                                                           >  1:664311‑M1/25‑163 (MQ=255)      agagtggtcttaagtttaatacctcaatgtgtatcacaatatcCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcg                                                                                                                                                      >  1:271648‑M1/20‑158 (MQ=255)                  agtttaatacctcaatgtgTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcac                                                                                                                                           >  1:415251‑M1/8‑146 (MQ=255)                              cAATGTGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagca                                                                                                                               >  1:574294‑M1/1‑134 (MQ=255)                               aaTGTGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGGGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCAACGAAGAGTTCAGGTAGTACAACGGCATGTCTgcaagaacctgacacttttgactatctggcaaccggccgtgtgttctctcacgcctcacaaaagcagcaagcataaaaaaacccgcctgtgcgggctttttaacaaaacttcagcaa                                                                                                                              >  1:622012‑M1/1‑133 (MQ=255)                                  gtttatcACAAGTTCCACATTCTTTGTAGGGG‑GTCTGGAGTTTGAGTAGATATGCTTGGTCAGAATTTATCAGTCGATGGTTCTACGATTCTTAAGCCACGAAGAGTGCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactagctggcaaccggcagtgtgtgttctcacgcatcacaaaagcagcatgcataataaaacccgcttgcgcgggctttttcacaaagcttcagcaaatgg                                                                                                                          <  2:395898‑M1/248‑122 (MQ=255)                                       tCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgat                                                                                                                     >  1:425040‑M1/1‑125 (MQ=255)                                             tatCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaa                                                                                                                                                                    <  2:525547‑M1/198‑80 (MQ=255)                                             tatCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaa                                                                                                                                                                    >  1:525547‑M1/1‑119 (MQ=255)                                                       cTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTc                                                                                                       >  1:228912/1‑250 (MQ=255)                                                                ggggtctGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggca                                                                                                                                                                                                                    <  1:399083‑M1/129‑32 (MQ=255)                                                                ggggtctGGAGATTGAGTAGATATTCTTGGTCAGAATGTCTCAGCCGATGGATCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGGCTgcaagaacctgcaacttttgactatctggca                                                                                                                                                                                                                    >  2:399083‑M1/4‑101 (MQ=255)                                                                      tGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcagg                                                                                       >  1:279774‑M1/1‑95 (MQ=255)                                                                      tGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggct                                                                                                                                                  >  1:296838‑M1/1‑95 (MQ=255)                                                                      tGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggct                                                                                                                                                  <  2:296838‑M1/192‑98 (MQ=255)                                                                       ggAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggt                                                                                      >  1:380824‑M1/1‑94 (MQ=255)                                                                                gTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggttagccttat                                                                             <  1:29387‑M1/251‑167 (MQ=255)                                                                                  aGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTCCGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctgtcaaccggcagagtgttctctcacgcatcacaaaagaagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaaaccagtttagtgatctgtgcagtcaggttagccttatg                                                                            >  1:550757‑M1/1‑83 (MQ=255)                                                                                            gTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggttagccttatgacgtgcagctt                                                                 <  1:652769‑M1/251‑179 (MQ=255)                                                                                                          aGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagagtgttctctcacgcatcccaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggt                                                                                      >  1:424275‑M1/1‑59 (MQ=255)                                                                                                                                     aCGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagggtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggttagccttatgacgtgcatctttgtttttgtggatcagacctttagcagcctgacggtccacg                        <  2:664311‑M1/251‑220 (MQ=255)                                                                                                                                        aagtgttcaAATAGTTCATCGTCATGTCTtcaagaacacgcaacttttgactatcgtgcaaccggcagtgggttctcttacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctgtttcacaaagcttcagcaaattggcgattaagccagttttttgatctgtgcagtcaggttagccttatgacgtgcagctttgtttttgtggatcagccctttagcagcctgacggtcctcgatc                     <  2:453913‑M1/247‑223 (MQ=255)                                                                                                                                         agagTTCAGATAGTACAACGGCATGTCTgcaagaccctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggttagccttatgacgtgcagctttgtttttgtggatcagacctttagcagcctgacggtccacgatcg                    <  1:268511‑M1/251‑224 (MQ=255)                                                                                                                                                         aaCGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggttagccttatgacgtgcagctttgtttttgtggatcagacctttagcagcctgacggtccacgatcggttgcatttcgttaaa    <  1:323731‑M1/251‑240 (MQ=255)                                                                                                                                                           cGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggctagccttatgacgtgcagctttgtttttgtgggtcagacctttagcagcctgacggtccacgatcggttgcgtttcgttaaatg  >  2:269916‑M1/1‑10 (MQ=255)                                                                                                                                                                    |                                                                                                                                                                                                                                                  AATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAA‑CCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGC  >  NC_000913/20540‑20943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.