Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,105,699	IS1 (+) +9 bp	coding (286‑294/312 nt)	ymdA →	uncharacterized protein YmdA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	290634 =	NA (NA)	50 (1.050)	45/486	0.1	98.0%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	1105699 =	1 (0.020)					coding (286/312 nt)	ymdA	uncharacterized protein YmdA
*	?	NC_000913	= 291401	NA (NA)	53 (1.110)	46/486	0.1	98.1%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 1105707	1 (0.020)					coding (294/312 nt)	ymdA	uncharacterized protein YmdA

TTCTTACAAAGGTGGAGGCAAACCCGTCCGTGTGTGAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGA  >  NC_000913/290394‑290882                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          ttcttACAAAGGTGGAGGCAAACCCGTCCGTGTGTGAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGTCGATATAGCTGTTACCGGGTAAT‑GAc                                                                                                                                                                                                                                                  <  1:199057/249‑1 (MQ=255)   cttACAAAGGTGGCGGCAAACCCGTCCGTGTGTGAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAGCATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTcc                                                                                                                                                                                                                                               >  2:464231/1‑250 (MQ=255)     tACAAAGGTGGAGGCAAACCCGTCCGTGTGTGAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTccta                                                                                                                                                                                                                                             <  1:353077/250‑3 (MQ=255)       cAAAGGTGGAGGCAAACCCGTCCGTGTGTGAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACtt                                                                                                                                                                                                                                          >  1:380280/1‑251 (MQ=255)                        ccgtccgtGTGTGAAAATAATCGTATCTGACTCAGCTTCTCTGCAGAAGCCGAAAGACATTGGCTCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGACTGATGCCCTTCTTCCTTACTTCAGCGGTGAAGGGGGAGATATTTCTGATCACGGGTCAT‑GACTCACACTTATTGATAGCGTTTTAtt                                                                                                                                                                                                                         >  2:201630/1‑250 (MQ=14)                            ccgggttgtaaaaTAATCGTATATGACTCCGATTCCCTGCTGAATCAGAAAGACATTGGATCCAAATCTACAAGCAGGTCGAGTCAGAAATGAGAATGAGTGGCCTTCTTGATGATTTTAAGGATTATGCTTCTTAAATGGCGGAGAACATTGCGAGGCTTGCGGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAG‑GACTCCCGCGTATTGATAGTGTTTTATGTTcc                                                                                                                                                                                                                     <  2:337748/242‑2 (MQ=14)                               tgtgagAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACACCCAGGTCGAGTCAGACATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACCTGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGAGGTGATGGAGGCGGTATTTCTGTTACCGGGTACT‑GACTCCAACTTATTGATGGTGTTTTATGTTCAGa                                                                                                                                                                                                                   >  2:228726/1‑250 (MQ=14)                                   tgAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAACTGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATg                                                                                                                                                                                                              >  1:350076/1‑251 (MQ=255)                                    gAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGc                                                                                                                                                                                                             <  1:389441/251‑1 (MQ=255)                                    gAAAAGAATCGTAACTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGGCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACGTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATg                                                                                                                                                                                                              <  2:63590/250‑1 (MQ=255)                                       gataatCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTCCAACCAGGTCGAGTCAGAAATGAGAATGATTGTCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTACTAGA‑TCCAACTTATTGGTAGAGTTTTATGTTCAGATAATGCCCg                                                                                                                                                                                                          >  2:396367/2‑251 (MQ=14)                                                           aTTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTc                                                                                                                                                                                      >  1:475909/1‑251 (MQ=255)                                                              ctctGCAGAAGCAGAAAGACATTGGATCGACTTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTTCTCCAc                                                                                                                                                                                   >  2:470636/1‑251 (MQ=255)                                                              ctctGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCAc                                                                                                                                                                                   >  1:8768/1‑251 (MQ=255)                                                                    agaagcagaaAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGAttt                                                                                                                                                                             <  1:440978/251‑1 (MQ=255)                                                                             aaGACATTGGATCGAATTCTACAACCAGGACGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCTGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGGTTTTGAGAACg                                                                                                                                                                     >  1:270486/1‑250 (MQ=255)                                                                                  aTTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGTTAATGCCCGATGACTTTTTCATGCTGCTCCACCGATTTTGAGAACGACAGCg                                                                                                                                                               >  2:3141/1‑251 (MQ=255)                                                                                         cGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTcc                                                                                                                                                         <  2:118098/250‑1 (MQ=255)                                                                                                    aCCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACT‑‑TTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGcc                                                                                                                                           >  1:419227/1‑251 (MQ=255)                                                                                                       aGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGcc                                                                                                                                           >  1:354674/1‑250 (MQ=255)                                                                                                       aGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCa                                                                                                                                          <  1:470636/251‑1 (MQ=255)                                                                                                          gcGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCAGTGCCAGGt                                                                                                                                       <  2:419903/250‑1 (MQ=255)                                                                                                                cAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGcc                                                                                                                                 >  1:375651/1‑251 (MQ=255)                                                                                                                   aaaTGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGACTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAAATTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCAGTCCCAGCCGTGCCAGGTGCTGCCTcc                                                                                                                              >  1:469251/1‑250 (MQ=255)                                                                                                                      tGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATTTCTGTTCCCGGGTAAT‑GACCCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGCCTTTGTCATGTTGCTCCACCGTTTTTGAGACCGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTc                                                                                                                               >  2:227802/1‑247 (MQ=25)                                                                                                                       gagaATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGACTCAGAtt                                                                                                                          >  1:202782/1‑251 (MQ=255)                                                                                                                        agaATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTTCTTCATTACATCAGCGGTGATGGAGGCGATATATCTGGTCACGGGGAAT‑GACTCCACCTTATTGATCGTGTTTTATGTTCAGATAAGGCCCGATGACTTTGGAATGCTGCTCCACCGATTTTGAGAACGACAACGACTACAGTCCCATCCGTGCCGGGTGCTGCCTCAAAtt                                                                                                                          >  2:313929/1‑250 (MQ=18)                                                                                                                                tGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGCTTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATg                                                                                                                 >  1:102475/1‑251 (MQ=255)                                                                                                                                           aTGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGCTAGTGTTTTATGTTCAGATAATGCCCGATGAATTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGGGCCCGGTGCTGCCTCCAATTCAGGATATGCCGCTCATTTc                                                                                                      >  2:439572/1‑251 (MQ=25)                                                                                                                                                        ttATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCg                                                                                         >  1:399873/1‑251 (MQ=255)                                                                                                                                                         tATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGCTAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGTTTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGc                                                                                        >  2:502913/1‑251 (MQ=255)                                                                                                                                                            gCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTg                                                                                     <  1:454477/251‑1 (MQ=255)                                                                                                                                                                                      gAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGACGGAGACGATATCTCTGTTACAGGGTAAT‑GACTCCAACTTATTGATAGGGTTTTATGTTCAGATAATGCCAGATGACTTTGTCATGCTTCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGAGCCCGGTGCTGCCTAAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCATGCTGATTACGTGCAGATTTCCCTTc                                                             >  2:31267/1‑249 (MQ=17)                                                                                                                                                                                            tgcttcCTTACTTCATTACTGCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACT‑‑TTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGAt                                                    <  2:419227/250‑1 (MQ=255)                                                                                                                                                                                                   ttacttcattacttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATAc                                              >  1:107520/1‑251 (MQ=255)                                                                                                                                                                                                     acttcattacttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACa                                             <  1:464231/250‑1 (MQ=255)                                                                                                                                                                                                     acttcattacttcaGCGGTGATGGAGGCGATATATCGTTTTCCGGGTAAT‑GACTCCAATTTATTGATATTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACTTGCAGCTTTCCCTTCAGTCGCGATTCAtcca                                             <  2:289612/250‑4 (MQ=14)                                                                                                                                                                                                                tcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGgccagcc                                   <  2:475909/249‑1 (MQ=255)                                                                                                                                                                                                                     ggTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCCGGCGGGATTCATACAGCGGCCAGCCATCCGTc                            >  2:17727/1‑251 (MQ=255)                                                                                                                                                                                                                       tgctGGAGGCGATATATCTGTTAACGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGATGCCTCAGATGCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGAAGCTTTCCCTTAAGGAGGGATTCATACAGCGGACAGCCATCCGTCa                           >  2:285962/4‑250 (MQ=11)                                                                                                                                                                                                                        gATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGACATc                         <  1:227802/251‑1 (MQ=255)                                                                                                                                                                                                                            gAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCat                      >  1:89841/1‑250 (MQ=39)                                                                                                                                                                                                                             gggCGATATATCTGTTACCGGTTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCatat                    <  2:380280/250‑1 (MQ=37)                                                                                                                                                                                                                             aGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCatat                    >  1:120995/1‑251 (MQ=38)                                                                                                                                                                                                                                   tatatCTGATACCGGGTAAT‑GACTCCAACGTATTGATAGTGTTTTATGATCAGATAATGCCCGATGACTTTGTCATGCTGCACCACCGATTTTGAGAACGACAGCGACTTCCGTCCTAGCCGTGCCAGGTGCTTCCTCAGATTCGGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGTGTACGTCCAGCTTGCCCTTCCGGCGGCGTTCATACAGCGGCCATCCATCCGTCATccctcacccaac              >  2:413902/1‑241 (MQ=11)                                                                                                                                                                                                                                    atatCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACg             <  1:157976/251‑1 (MQ=35)                                                                                                                                                                                                                                         gatacgcGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCaa         >  1:20905/7‑250 (MQ=11)                                                                                                                                                                                                                                           ttACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAgg      >  1:371740/1‑251 (MQ=32)                                                                                                                                                                                                                                            tACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAggg     >  2:318363/1‑251 (MQ=31)                                                                                                                                                                                                                                              aggggTAAT‑GACTCCATGTTATTGATATTGTTTTATGCTCAGATAATGCCCGACGACTTTGTCCTGCTGCTACACCGATTGTGATAACGACAACGCCTTCAGTCCCTGCCGTGCCAGGTGCTGCCCTAGATACAGGTTATTCCGCTCAATCTGTTGCGTATATCGCTTGCTGATTACGTGCTGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCAGTCATCCATATCACCACGTCAAAgtgt    <  1:392994/248‑4 (MQ=11)                                                                                                                                                                                                                                               cGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCAGGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGa  <  1:356779/251‑1 (MQ=11)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          TTCTTACAAAGGTGGAGGCAAACCCGTCCGTGTGTGAAAATAATCGTATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAAT‑GACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGA  >  NC_000913/290394‑290882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.