Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	20,703	+GCAAGAACCTGCAA	intergenic (‑195/+112)	insA1 ← / ← rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 20703	3 (0.100)	15 (0.550) +GCAAGAACCTGCAA	11/460	1.0	84.1%	intergenic (‑195/+112)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20
	?	NC_000913	20704 =	3 (0.100)					intergenic (‑196/+111)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

CTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCT‑‑‑‑‑‑‑‑‑‑‑C‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGT  >  NC_000913/20537‑20951                                                                                                                                                                                   |                                                                                                                                                                                                                                                                  aaaaatatagtggtcttaagtttaatacctcaatgtgtatcacaatatccacattctttgtatggggtctggagattgagtagatattcttgttcagaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatttctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCt                                                                                                                                                                                  <  1:358690‑M2/73‑1 (MQ=255)             gtcttaagtttaatacctcaatgtgtatcacaatatccacattctttgtagggggtctggagattgagtagatattcttgttcagaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCttt                                                                                                                                                                      >  1:41731‑M2/167‑251 (MQ=255)              tcttaagtttaatacctcaatgtgtatcacaatatccacattctttgtagggggtctggagattgagtagatattcttgttcagaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCtttt                                                                                                                                                                     >  2:133827‑M2/166‑251 (MQ=255)               cttaagtttaatacctcaatgtgtatcacaatatccacattctttgtagggggtctggagattgagtagatattcttgttcagaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCttttt                                                                                                                                                                    >  1:375565‑M2/165‑251 (MQ=255)                 taagtttaatacctcaatgtgtatcacaatagccacattctgtgtaggggttctggagattgagtagatattcttgttcagaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCTGGCttttt                                                                                                                                                                    <  2:375095‑M2/87‑1 (MQ=255)                      ttaatacctcaatgtgtatcacaatatccacattctttgtagggggtctggagattgagtagatattcttgttcagaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACaaa                                                                                                                                                              <  1:202983‑M2/93‑1 (MQ=255)                                         cacaatatccacattctttgtagggggtctggagattgagtagagattcttgttcagaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGAt                                                                                                                                          <  1:220783‑M2/112‑1 (MQ=255)                                         aTCACAATATCCACATTCAATGAAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGACACGAAGAGTTCAAATAGTACAACGGCATGTCTGAAAGAAC‑‑‑C‑‑‑TGCAACATTTGACTATCGGGCAACCGTACGTGTGTTCTCTACCGCCTCACAAAAGCAGCAGGCATAACCAAAACCGCTTGAGCGGGCTTATTCACCAAGCTACAG‑CAAATTGGCg                                                                                                                                            >  2:304890/1‑250 (MQ=255)                                                tccacattctttgtagggggtctggagattgagtagatattcttgttcagaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACACGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGcc                                                                                                                                    >  1:27391‑M2/132‑249 (MQ=255)                                                                 gggtgtggagattgagtagatattcttgttcaaaatgtatcagccgatggttctacgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAg                                                                                                                 <  2:41731‑M2/137‑1 (MQ=255)                                                                     cggggaatttagtagatattctttttaggattgtattagccgatggttctacgattcttaagccacgaagatttcatatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCGTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAg                                                                                                             <  2:17094‑M2/141‑1 (MQ=255)                                                                           gagaTTGAGTAGATATTCTTGTTCCGAATGTATCCGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTGCAAGAACATGCAAAT‑‑‑‑‑‑TTTGACTATCTGGCAACAGGCAGTGAGTTCTCTCACGCACCACAAAAGCAGCAAGACTAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAACTTTAGACAAAT‑GGCGATTAAGACAGGTATTTGATCTGTGACGTCAGGtt                                                                                                          >  2:40447/1‑251 (MQ=255)                                                                                                aacagaatgttccagccaaggtttttACGATGCTTAATCCACGATTAGTTCAGATAGTACAATGGCCTGTCG‑‑‑‑‑‑‑‑‑‑‑C‑‑‑T‑‑‑‑‑‑TTTTACTATCTGGTAAACGGAAGTGTGTTCTCTCTCTCATCACAAAAGCAGCAGGCATAAAAAAACCCGCGTGCGCGGGCGTTTTCACTAAGCTTCAG‑CAAATGTGCTATTAAGCCAGTTTGTTGATCTTTGCATGCTGGTTAGCCTTATGTCGTGCAGCTTTGTTTTTGTTGATCa                                                                       <  2:327243/226‑1 (MQ=255)                                                                                                               atgtttctgcgttttttaagccacgaagagtttagatagtacaacggaatgtcttcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑GTTGACGATCGGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGTGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCGGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTATGTGGATCAGAcc                                                                   <  2:113477‑M2/183‑1 (MQ=255)                                                                                                                       acgattcttaagccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGGGTTCTCTCACGCATCAAAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATg                                                                                                 <  1:153903‑M2/153‑1 (MQ=255)                                                                                                                                  gccacgaagagttcagatagtacaacggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTcc                                                <  1:90690‑M2/202‑1 (MQ=255)                                                                                                                                              tcagatagtacaacggcaagtctgcaagaacctgcaaC‑‑‑A‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGATCTCTCACGCATCAAAAAAGCAGCAGGCATCAAAAAACACGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTTACCTTCTGACGTGCTGCTTTGTTTTTGTGGTAAAGACTTTTAGCATCCTGTCGGTCCAcgatcgtgtgt                                    >  2:72442‑M2/38‑246 (MQ=255)                                                                                                                                                            ggcatgtctgcaagaacctgcaaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGc                      <  1:133827‑M2/228‑1 (MQ=255)                                                                                                                                                                               gcaaC‑‑‑T‑‑‑‑‑‑GTTTACTATCTGGCAACCGGCAGTGTGTTCGCCCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTTTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCtt     <  2:188795‑M2/245‑1 (MQ=255)                                                                                                                                                                                caaC‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGt  <  1:304890‑M2/248‑1 (MQ=255)                                                                                                                                                                                   |                                                                                                                                                                                                                                                                  CTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCT‑‑‑‑‑‑‑‑‑‑‑C‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGTTAAATGCTTTCTGTGCAGCAGCTTTGT  >  NC_000913/20537‑20951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.