Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	20,703	+GCAAGAACCTGCAA	intergenic (‑195/+112)	insA1 ← / ← rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 20703	3 (0.100)	15 (0.550) +GCAAGAACCTGCAA	11/460	1.0	84.1%	intergenic (‑195/+112)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20
	?	NC_000913	20704 =	3 (0.100)					intergenic (‑196/+111)	insA1/rpsT	IS1 family protein InsA/30S ribosomal subunit protein S20

AAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCT‑‑‑‑‑‑‑‑‑‑‑C‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGT  >  NC_000913/20539‑20924                                                                                                                                                                     |                                                                                                                                                                                                                                                   aaaaatatagtggtcttaagtttaatacctcaatgtgtatcacaatatccacattCTTTGTATGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATTTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgct                                                                                                                                                             <  1:358690‑M1/226‑88 (MQ=255)             gtcttaagtttaatacctcaatgtgtatcacAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggcttt                                                                                                                                                 >  1:41731‑M1/14‑152 (MQ=255)              tcttaagtttaatacctcaatgtgtatcaCAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttt                                                                                                                                                >  2:133827‑M1/13‑151 (MQ=255)               cttaagtttaatacctcaatgtgtatcACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggcttttt                                                                                                                                               >  1:375565‑M1/12‑150 (MQ=255)                 taagtttaatacctcaatgtgtaTCACAATAGCCACATTCTGTGTAGGGG‑TTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgctggcttttt                                                                                                                                               <  2:375095‑M1/240‑102 (MQ=255)                      ttaatacctcaatGTGTATCACAATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaa                                                                                                                                         <  1:202983‑M1/246‑108 (MQ=255)                                       aTCACAATATCCACATTCAATGAAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGACACGAAGAGTTCAAATAGTACAACGGCATGTCTGAAAGAAC‑‑‑C‑‑‑TGCAACATTTGACTATCGGGCAACCGTACGTGTGTTCTCTACCGCCTCACAAAAGCAGCAGGCATAACCAAAACCGCTTGAGCGGGCTTATTCACCAAGCTACAG‑CAAATTGGCg                                                                                                                 >  2:304890/1‑250 (MQ=255)                                         cacaATATCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGAGATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgat                                                                                                                      <  1:220783‑M1/250‑127 (MQ=255)                                                tCCACATTCTTTGTAGGGG‑GTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacacgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagcc                                                                                                                >  1:27391‑M1/1‑117 (MQ=255)                                                                  gggtgtggagaTTGAGTAGATATTCTTGTTCAAAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcag                                                                                             <  2:41731‑M1/246‑152 (MQ=255)                                                                      cggggaatttagtagatattcTTTTTAGGATTGTATTAGCCGATGGTTCTACGATTCTTAAGCCACGAAGATTTCATATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcgtgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcag                                                                                         <  2:17094‑M1/241‑156 (MQ=255)                                                                         gagaTTGAGTAGATATTCTTGTTCCGAATGTATCCGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTGCAAGAACATGCAAAT‑‑‑‑‑‑TTTGACTATCTGGCAACAGGCAGTGAGTTCTCTCACGCACCACAAAAGCAGCAAGACTAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAACTTTAGACAAAT‑GGCGATTAAGACAGGTATTTGATCTGTGACGTCAGGtt                                                                               >  2:40447/1‑251 (MQ=255)                                                                                              aacagaatgttccagccaaggtttttACGATGCTTAATCCACGATTAGTTCAGATAGTACAATGGCCTGTCG‑‑‑‑‑‑‑‑‑‑‑C‑‑‑T‑‑‑‑‑‑TTTTACTATCTGGTAAACGGAAGTGTGTTCTCTCTCTCATCACAAAAGCAGCAGGCATAAAAAAACCCGCGTGCGCGGGCGTTTTCACTAAGCTTCAG‑CAAATGTGCTATTAAGCCAGTTTGTTGATCTTTGCATGCTGGTTAGCCTTATGTCGTGCAGCTTTGTTTTTGTTGATCa                                            <  2:327243/226‑1 (MQ=255)                                                                                                                atgtttctgcgttttttaagccacgaagagttTAGATAGTACAACGGAATGTCTtcaagaacctgcaactgttgacgatcgggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgtgctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatcggtgcagtcaggttagccttatgacgtgcagctttgtttatgtggatcagacc                                               <  2:113477‑M1/236‑198 (MQ=255)                                                                                                                        aCGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgggttctctcacgcatcaaaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggttagccttatg                                                                             <  1:153903‑M1/213‑168 (MQ=255)                                                                                                                                   gCCACGAAGAGTTCAGATAGTACAACGGCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggttagccttatgacgtgcagctttgtttttgtggatcagacctttagcagcctgacggtcc                            <  1:90690‑M1/251‑217 (MQ=255)                                                                                                                                               tCAGATAGTACAACGGCAAGTCTgcaagaacctgcaacatttgactatctggcaaccggcagtgtgatctctcacgcatcaaaaaagcagcaggcatcaaaaaacacgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggtttaccttctgacgtgctgctttgtttttgtggtaaagacttttagcatcctgtcggtccacgatcgtgtgt                >  2:72442‑M1/1‑23 (MQ=255)                                                                                                                                                             ggCATGTCTgcaagaacctgcaacttttgactatctggcaaccggcagtgtgttctctcacgcatcacaaaagcagcaggcataaaaaaacccgcttgcgcgggctttttcacaaagcttcagcaaattggcgattaagccagtttgttgatctgtgcagtcaggttagccttatgacgtgcagctttgtttttgtggatcagacctttagcagcctgacggtccacgatcggttgcatttcgttaaatgc  <  1:133827‑M1/251‑243 (MQ=255)                                                                                                                                                                     |                                                                                                                                                                                                                                                   AAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACAATATCCATATTCTTTGTGGGGGAGTCTGGAGATTGAGTAGATATTCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCT‑‑‑‑‑‑‑‑‑‑‑C‑‑‑T‑‑‑‑‑‑TTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAG‑CAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTGCATTTCGT  >  NC_000913/20539‑20924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.