Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,586,576	IS5 (–) +4 bp	intergenic (‑90/+241)	ydeP ← / ← ydeQ	putative oxidoreductase YdeP/putative fimbrial adhesin protein YdeQ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1396044 =	NA (NA)	24 (0.820)	24/488	0.2	95.9%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	1586576 =	1 (0.030)					intergenic (‑90/+244)	ydeP/ydeQ	putative oxidoreductase YdeP/putative fimbrial adhesin protein YdeQ
*	?	NC_000913	= 1397238	NA (NA)	20 (0.690)	19/486	0.5	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	= 1586579	0 (0.000)					intergenic (‑93/+241)	ydeP/ydeQ	putative oxidoreductase YdeP/putative fimbrial adhesin protein YdeQ

TGGAATAAAAATCCTTTTCTTTTACGATTTCCACGGCGAAACCAAAAGCTCATCAGAATCAATGCTGCGCAGCTACCGAAAATAATGACCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCT  >  NC_000913/1395795‑1396281                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              tGGAATAAAAATCCTTTTCTTTTACGATTTCAACGGCGAAACCAAAAGCTCATCAGAATCAATGCTGCGCAGCTACCGAAAATAATGACCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGCCAATTCATTAAGATCATCAATA‑GACAGGGAAAGAAGTAGCAAAATTAGCTaagg                                                                                                                                                                                                                                              >  1:292226/1‑251 (MQ=255)            tCCTTTTCTTTTACGATTTCCACGGCGAAACCAAAAGCTCATCAGAATCAATGCTGCGCAGCTACCGAAAATAATGACCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAAc                                                                                                                                                                                                                                   >  1:64057/1‑251 (MQ=255)                     tttACGATTTCCACGGCGAAACCAAAAGCTCATCAGAATCAATGCTGCGCAGCTACCGAAAATAATGACCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTAGTCATGCCGGAAACCCCTGGCGGGGCTATTTTCCCGCGACAATTCATTCAGATCCTCAATA‑GTCGGGGAAGGAAGTAGCCACATTAGCTAAGGAAGGTGCGAACAAGTCCCTg                                                                                                                                                                                                                          >  1:28801/1‑251 (MQ=255)                       tACGATTTCCACGGCGAAACCAAAAGCTCATCAGAATCAATGCTGCGCAGCTACCGAAAATAATGACCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAAAATTAGCTAAGGAAGGAGCGCACAAGTCCCTGat                                                                                                                                                                                                                        >  1:377684/1‑251 (MQ=255)                             ttCCACGGCGAAACCAAAAGCTCATCAGAATCAATGCTGCGCAGCTACCGAAAATAATGAACAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGGTATTTTACCGCGACAATTCATTCAGGTCATCAATA‑GTCAGGGAAGGAAGGAGCAACATAAGCTAAGGAAGGGGCGAACAAGTCCCTGattagagg                                                                                                                                                                                                                  >  2:19567/1‑245 (MQ=255)                                            aaaaGCACATCAGAATCAATGCTGCGCAGCTACCGCAAATAATGACCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCCATTTTACCGCGACAATTCCTTCAGATCATCAATA‑GTCCGGGAAGGAAATAGCAACATACGATTAGGACGGTGCGACAAAGTCCCttgaattggatcaaatttttgatct                                                                                                                                                                                                   >  2:199648/1‑227 (MQ=255)                                             aaaGCTCATCAGAATCAATGCTGCGCAGCTACCGAAAATAATGACCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTg                                                                                                                                                                                                  <  1:19567/251‑1 (MQ=255)                                                                                        aCCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGAt                                                                                                                                                                                                                 >  1:218847/1‑193 (MQ=255)                                                                                        aCCAGATGAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCAGGCCTGAAACCCCTTGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGAt                                                                                                                                                                                                                 <  2:218847/193‑1 (MQ=255)                                                                                                 aGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGt                                                                                                                                              >  1:98023/1‑251 (MQ=255)                                                                                                                       cAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCt                                                                                                                                                         <  1:272103/218‑1 (MQ=255)                                                                                                                       cAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTAATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAATCCCTGATATGAGATCATGTTTGTCATATGGAGCCATAGAACAAGGTTCATAATGAGTAATCAACTTACCt                                                                                                                                                         >  2:272103/1‑218 (MQ=255)                                                                                                                          tgattcatgttgtATCAAGTTCCTCCAGTTAATTGTCGTCATGCCGGAAACTCCTGGCAGGGCTATTTTACCGCAACAATTCATTCAGATCATCAATAACTC‑GGGAAGGAAGTAGCCACATTAGCTAAGGGAGGTGCGAACCAATCCCTGATATGAGGTGTGGATTGTCATCTGTAGCCATAGAAAAGGGTTCATCATGAGTCATCAACTTACCTTCGCTCACAGTGAAATcagcaagaagggcagttcg                                                                                                                      >  2:90101/8‑236 (MQ=16)                                                                                                                          cGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGAGACAATTCATTCAGATCATAAATA‑GGCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCCTAGAACAGGGTTCATCATGAGTCATCACATTACCTTCGCCGCCAGAGAATTGAGCAGTAAGCGCAGTc                                                                                                                        >  2:73451/1‑248 (MQ=25)                                                                                                                               aTTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAAATCATTCAGAGCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGa                                                                                                                <  2:117019/251‑1 (MQ=255)                                                                                                                                           ggCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAAGTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTc                                                                                                    <  1:60231/251‑1 (MQ=255)                                                                                                                                                 tccAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCAATTTTACCGCGACAATTCATTCAGAACATCAATA‑GACAGGGAAGGCAGAAGCAACATTAGCTAAGGAAGGGGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCAAGAGTCATCAACTTAACATCGCCGACAGTGAATTCAGCAGTAAGCGCCGTAAGACCAGATAAGAGATTTTCATGTcc                                                                                              >  1:171843/1‑251 (MQ=21)                                                                                                                                                  ccAGATAATTGGCGTCATGCCGGAAACCCCGGGCGGGGCTATTTTACCGCGACAATGCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTccc                                                                                             <  2:98023/251‑1 (MQ=255)                                                                                                                                                                         aaaCCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATgg                                                                      <  1:37282/251‑1 (MQ=255)                                                                                                                                                                                 ggCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCtt                                                                                                  <  2:146513/215‑1 (MQ=255)                                                                                                                                                                                 ggCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCtt                                                                                                  >  1:146513/1‑215 (MQ=255)                                                                                                                                                                                     gggatATTTTACCGCGACACTTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATAAGAGATCATGTTTGTCATATGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTcagc                                                                                                                                     >  2:124719/5‑176 (MQ=255)                                                                                                                                                                                     gggCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTcagc                                                                                                                                     <  1:124719/176‑1 (MQ=255)                                                                                                                                                                                        cTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAAAATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGa                                                        >  1:132748/1‑250 (MQ=255)                                                                                                                                                                                                            attcaGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTc                                                                              <  1:243482/208‑1 (MQ=255)                                                                                                                                                                                                            attcaGATCATCAATA‑GTAAGGGAAGGAAGTAGCAACATTAGATAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATAAACTTACATTCGCTGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGCTTTTCTTGTACCGCGTGGAGCAGCTTc                                                                              >  2:243482/1‑208 (MQ=17)                                                                                                                                                                                                              tcaGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTAcccc                                  <  1:196101/250‑1 (MQ=255)                                                                                                                                                                                                                aGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCa                                 >  1:338285/1‑249 (MQ=255)                                                                                                                                                                                                                                agggacggaatgaGCAACATTAGCTAAGGAAGGTGCGAAAAAGTCCCTGATATGAGATCATGTTTGTCATCTGGCGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACACCAAGGCTGGTAATGGCCgg                <  2:64057/239‑1 (MQ=255)                                                                                                                                                                                                                                  ggaaggaaGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGCTTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGTACGTCATCGAGCCGTTTTACCCGAAGTCTGGAAATGACCGGcc              >  2:331431/1‑250 (MQ=14)                                                                                                                                                                                                                                    aaggaagTAGCAACATTAGCTAAGGAAGGGGCGAACAAGTCCCTGATATGAGATCATGTTTGTCAGCTGGAGCCATAGAACAGGGTTCATCATTAGTCATCAACTTACCTTCGTCGACAGTGAATTCAGCAGTAAGCTCCTTCAGACCAGAAAAGAGATTTGCTTGTCCCGCATGGAGCAGATTCTGCCATTGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGAc            <  1:246025/251‑1 (MQ=11)                                                                                                                                                                                                                                              ccaCATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGCGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCt  >  2:37436/3‑251 (MQ=17)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              TGGAATAAAAATCCTTTTCTTTTACGATTTCCACGGCGAAACCAAAAGCTCATCAGAATCAATGCTGCGCAGCTACCGAAAATAATGACCAGATTAAGCGCATTATTTGTAAACAGTTCAGCGATCATTGTTTTATCAGGCTCCTCCAGATAATTGTCGTCATGCCGGAAACCCCTGGCGGGGCTATTTTACCGCGACAATTCATTCAGATCATCAATA‑GTCAGGGAAGGAAGTAGCAACATTAGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCT  >  NC_000913/1395795‑1396281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.