Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,124,498	IS2 (+) +5 bp	intergenic (‑42/+110)	cytR ← / ← priA	DNA‑binding transcriptional repressor CytR/primosomal protein N'

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4124498 =	2 (0.070)	29 (0.990)	29/488	0.1	93.4%	intergenic (‑42/+114)	cytR/priA	DNA‑binding transcriptional repressor CytR/primosomal protein N'
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4124502	1 (0.040)	25 (0.860)	24/486	0.2	96.1%	intergenic (‑46/+110)	cytR/priA	DNA‑binding transcriptional repressor CytR/primosomal protein N'
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

GGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4124256‑4124502 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCC  >  NC_000913/4498181‑4498400                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     GGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGA                                                                                                                                                                                                                          <  1:172438/251‑1                  CGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTC                                                                                                                                                                                                         >  2:116295/1‑251                      GCGCCCCATAGGCTGCTGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAG                                                                                                                                                                                                      <  1:266515/250‑1                      GCGCCCCATAGGCTGCGGTAAACAACCCGCTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAG                                                                                                                                                                                                      <  1:190207/250‑1                          CCCATAGGCTGCGGTAAATAACCCACTTCCCTGGCCGCGTTTTCAACCCGATTACGGGTGGCCTAGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAAC                                                                                                                                                                                                 <  1:263914/251‑1                                             AACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACCCTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAcgt                                                                                                                                                                              >  1:29368/1‑248                                                           GCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATACATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTATACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGAGATAGTCTTAATAC                                                                                                                                                                >  1:280350/1‑251                                                              GCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGACTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCAACCTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGCCTGGCCCCCTGAATCTCCAGACAACCAATATCCATTAAATAAGTGATAGTCTTAATACT                                                                                                                                                               >  2:304084/1‑249                                                                      AACCCGAGTACGGGTTGCCTGGGAGACTTTATCGTGATGCAGTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGA                                                                                                                                                     <  2:106388/251‑1                                                                           GATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACT                                                                                                                                                   >  1:254582/1‑248                                                                                 GGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG                                                                                                                                          >  1:310215/1‑251                                                                                                             CATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCC                                                                                                               <  1:304084/250‑1                                                                                                                         GGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGA                                                                                                  <  1:136885/251‑1                                                                                                                          GAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGAC                                                                                                 <  1:349558/251‑1                                                                                                                             ACGGTCGCTGTAGCGACTTTTGCCTTGAAGGCAACGTCTTTAATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACCCTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTCGACTGGCCCCCTGAATATCCAGACAACCAATATCACTTAACTAAGAGATAGTCTTAATACTAGATTATAGACTAGTCATTGGAGAACAAATGGTTGATGTCTTAGGGCCGGACAAACGAAGACGGg                                                                                              >  2:316068/1‑250                                                                                                                              CGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGATTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGCAAACCAATATCACTTAAATAAGAGATAGTCTTAATAATAGTTTTTAGACTAGTCATTGGAGAAAAGATGATTGCTGTCTTAGGGCCGGAGAAACGCAGACGacg                                                                                             >  2:228624/1‑248                                                                                                                              CGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTGTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCG                                                                                             <  1:35461/251‑1                                                                                                                                                  GCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAA                                                                               >  1:321043/1‑245                                                                                                                                                      TGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAAT                                                                     >  1:27473/1‑251                                                                                                                                                      TGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCA                                                                       <  1:116295/249‑1                                                                                                                                                          GGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGCTGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTT                                                                 >  1:381620/1‑251                                                                                                                                                                                                      TCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCC                      <  1:76210/250‑1                                                                                                                                                                                                                 ccgCGCCTGGCACGTCAGTCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTG          <  1:228624/248‑1                                                                                                                                                                                                                 CCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTG          >  1:268646/1‑251                                                                                                                                                                                                                         gtcatggcaggcggtctcCATCTATGTTTACTAGCCTGGCCCCCTGAATCTCCAGACAACCACTATCACTTAAATAAGTGATAGTCTTAATACTAGTTGTTAGACTAGTCATTGGAGAACAGATGATTGATGTCGTAGGGCTGGAGAAACGCAGACGGCGTACCTCACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGTACCGGGGATGTCGTTCTCCCTCGTTTACCGGCATCATGGTGTAGCAGCC  <  2:254582/233‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     GGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTTTGCCTTGAGGGCAACGTCTTTCATGGTCGCGGCAGTTTCCTGCTTCTTCGCTTTCACACTCACTCCTCGCCTGGCACGTCAGGCGTACTACATCCATGTTTACt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4124256‑4124502 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCC  >  NC_000913/4498181‑4498400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.