Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,105,699	IS1 (+) +9 bp	coding (286‑294/312 nt)	ymdA →	uncharacterized protein YmdA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	290634 =	NA (NA)	31 (1.060)	28/488	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	1105699 =	0 (0.000)					coding (286/312 nt)	ymdA	uncharacterized protein YmdA
*	?	NC_000913	= 291401	NA (NA)	12 (0.410)	12/488	1.0	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 1105707	0 (0.000)					coding (294/312 nt)	ymdA	uncharacterized protein YmdA

CAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/291154‑291401 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGGAATAGAACCATTCTGTGGGCAGTTCATTTCTACGGGTGATGACTGGGTGCTGACGTCACATGCCGGTTCCACAATTTGGCCATAAAAATGGATCACGCCCCCTTGCACGGTGCTTCCAGCAATGGCAATAAAAGG  <  NC_000913/1105707‑1105462                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                CAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAT                                                                                                                                                                                                                                                     <  1:181842/251‑1                                           CGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGTCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTAC                                                                                                                                                                                                           <  1:211843/251‑1                                                            TAAGTGAACTGCAGCTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCAGTGCTTTTGCCGTTAAGCACCAACCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACAGCACTTTTACTTAGGCTATTTGCGCGT                                                                                                                                                                                          >  1:310120/1‑251                                                                                                             GCTGATGTACGGCAGAGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGCACAGGAGGGACAGCTGATAGAAACAGAAGCCACTAGAGCACCTCAAAAACACCCTCATACACTAAATCAGTAAGTTGGCAGCATCACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGG                                                                                                                                         >  2:197041/1‑251                                                                                                                 gtggCCGGCAGTGCGTTTGCCGTTACGCGCCTCCCCGTCAGGAGCTGAACAGGAGGGACAGCTGATAGAATCAGAAGCCGCTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCGCCCATTACAGCCAGCTTTTTCTGTTTATTAAGATACTGCACATTTACTGAGGCTATTTGCGCGGTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGGAATA                                                                                                                                     <  1:113899/247‑1                                                                                                                    TCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTCCTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGGAATAGA                                                                                                                                   >  2:338327/1‑250                                                                                                                        GCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGGAATAGAACCAT                                                                                                                              <  1:7572/251‑1                                                                                                                                                        AGTAGCTGAACAGGAGGTACAGCTGATAGAAACCGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTATATCAGTAAGTTGGCAGCATCCCCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTTGAGTAGGTTTTGCCCGGAATAGAACCATTCTGTGGGCAGTTCATTTCTACGGGTGATGAC                                                                                              <  1:358974/251‑1                                                                                                                                                                    GGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGGAGGTTTTGCCCGGAATAGAACCATTCTGTGGGCAGTTCATTTCTACGGGTGATGACTGGGTGCTGACG                                                                                  <  1:217262/251‑1                                                                                                                                                                                            AGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGGAATAGAACCATTCTGTGGGCAGTTCATTTCTACGGGTGATGACTGGGTGCTGACGTCACATGCCGGTTCCACAATTTGG                                                          <  1:169906/251‑1                                                                                                                                                                                                ACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGGAATAGAGCCATTCTGTGGGCAGTTCATTTCTACGGGTGATGACTGGGTGCTGACGTCACATGCCGGTTCCACAATTTGGCCAT                                                      <  1:37507/251‑1                                                                                                                                                                                                                                                     CACCCATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGCGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGGCATAGAACCATTCTGTGGGCAGTTCATTTCTACGGGTGATGACTGGGTGCTGACGTCACATGCCGGTTCCACAATTTGGCCATAAAAATGGATCACGCCCCCTTGCACGGTGCTTCCAGCAATGGCAATAAAAGG  >  1:380942/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                CAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/291154‑291401 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATTACAGCCAGCTTTTTCTGTTTATCAAGATACTGCACTTTTACTGAGGCTATTTGCGCGTTTTTGACATTGCCGCTCATTAACGCTTTGCTGGAGTAGGTTTTGCCCGGAATAGAACCATTCTGTGGGCAGTTCATTTCTACGGGTGATGACTGGGTGCTGACGTCACATGCCGGTTCCACAATTTGGCCATAAAAATGGATCACGCCCCCTTGCACGGTGCTTCCAGCAATGGCAATAAAAGG  <  NC_000913/1105707‑1105462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.