Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	59,092	(15-bp)2→1	coding (619‑633/651 nt)	yabP →	putative uncharacterized protein YabP

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	59090 =	2 (0.060)	13 (0.450)	13/446	1.1	87.1%	coding (617/651 nt)	yabP	putative uncharacterized protein YabP
	?	NC_000913	= 59074	2 (0.070)					coding (601/651 nt)	yabP	putative uncharacterized protein YabP

CTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTGATCTTAGAAAGGCTTACCTTCCCGGCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCAC  >  NC_000913/58889‑59340                                                                                                                                                                                                          |                                                                                                                                                                                                                                                           agaataagatatgtactcagacgttgcagctgcataagggccaaggtaacttgggcgttgatcttagaaaggcttaccttcccggcgttgacatgagggattgttaccttggtaaaaaaacaatgaaaggtagcaatgatatcctttatgagagacctgggtggaatgctaacctgggcgtgctaccccggacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTAcctcct                                                                                                                                                                                                           >  2:76163‑M1/202‑251 (MQ=255)           gCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTGATCTTAGAAAGGCTTACCTTCCCGGCGTTGACATGAGTGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATatgatg                                                                                                                                                                                                  <  2:158344/250‑1 (MQ=255)              tactcagaagctgcagctgcataagggccaaggtaacttgggcgttgatcttagaaaggcttaccttcccggcgatgacatgagggattgttaccttggtaaaaaaacaatgaaaggtagcaatgatatcctttatgagagacctgggtggaatgctaacctgggcgtgctaccccggacggtgctacCCCGGACGGGGCTAACCTGGACGGTGCTACCGTTACAGGTGCTACCTCCTTATATGATGAGGt                                                                                                                                                                                              <  2:214799‑M1/63‑1 (MQ=255)                 tcagacgttgcagctgcataagggccaaggtaacttgggcgttgatcttagaaaggcttaccttcccggcgttgacatgagggattgttaccttggtaaaaaaacaatgaaaggtagcaatgatatcctttatgagagacctgggtggaatgctaacctgggcgtgctaccccggacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTCCCTCCTTATATGatgaggaaat                                                                                                                                                                                           <  2:276999‑M1/66‑5 (MQ=255)                                 aaCTTGGTGGTTTATCGTAGAAAGGGGTACTTTCCCGGCGTTGACATGAGGGATTGTTAGCTTGGTAAAGAAACAAGGAAAGGGATCAATGATATCCTTTATGAGAGACCTTGGGGGAATGCTAACCTTGGCGTGCTACCCCGGATGGTGCTACCCCG‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑ACGGTGCTAATCTGGACGGTGCTACCGGTAACGGTGCTTCCTCCTTAGATGATGAGGTAATTATTATTAATAAAATCCCCCCCaaaaaaagt                                                                                                                                                            <  2:344141/251‑3 (MQ=255)                                                                                ccccggcggtgacatgagggattgttaccttggtaaaaaaacaattaaaggtagcaattatatcctttatgagagacctggctggaatgctaacctgggcgtgctaccccggacggtgcttaCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGc                                                                                                                            >  2:302307‑M1/123‑251 (MQ=255)                                                                                                         taccttggtaaaaaaacaatgaacggtagcaatgatatcctttatgagagacctgggtggaatgctaacctgggcgtgctaccccggacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACaa                                                                                                    <  1:262051‑M1/153‑1 (MQ=255)                                                                                                               ggtaaaaaaacaatgaaaggtagcaatgatatcctttatgagagacctgggtggaatgctaacctgggcgtgctaccccggacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTg                                                                                             >  1:276999‑M1/92‑251 (MQ=255)                                                                                                                                 ggtagcaatgatatcctgtatgagagacctgggtggaatgctaacctgggcgtgctaccccggacggtgctccCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAAt                                                                           >  2:205116‑M1/74‑251 (MQ=255)                                                                                                                                                            cctgggtggaatgctaacctgggcgtgctaccccggacggtgctacCCCGGACGGCGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAggg                                                <  1:160760‑M1/205‑1 (MQ=255)                                                                                                                                                             ctgggtggaatgctaacctgggcgtgctaccccggacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCACCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGAACTGATGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCAAAGGTTGAAgggg                                               <  2:319505‑M1/206‑1 (MQ=255)                                                                                                                                                                               tgggcgtgctaccccggacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAAc                             <  1:213318‑M1/224‑1 (MQ=255)                                                                                                                                                                               ggggcgtgctaccccggacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAATGGTGCTACCTCCTTATATGATGAGGGAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTTCTGAACAAATTACTGACAACGCAATTATTTAATGAGCCAGAAGAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAAc                             >  2:197971‑M1/28‑251 (MQ=255)                                                                                                                                                                                               gacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATaaa                                                                                                                                                                              >  1:345200‑M1/12‑90 (MQ=255)                                                                                                                                                                                               gacggtgctacCCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATaaa                                                                                                                                                                              <  2:345200‑M1/79‑1 (MQ=255)                                                                                                                                                                                                          cccggacggtgctaaccTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAAACCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTcac  <  2:213318/251‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                                                           CTCAGACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTGATCTTAGAAAGGCTTACCTTCCCGGCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGATATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTACCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGAACGGTGCTACCTCCTTATATGATGAGGTAATTATTATTAATAAAATCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCAC  >  NC_000913/58889‑59340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.