Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,560,081 IS1 (+) +9 bp intergenic (+215/+533) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3560081 =1 (0.040)53 (1.830) 46/484 0.0 98.1% intergenic (+215/+541) rtcR/glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
?NC_000913 = 3584195 NA (NA)noncoding (768/768 nt) IS1 repeat region
* ? NC_000913 = 35600891 (0.040)30 (1.040) 27/484 0.2 96.7% intergenic (+223/+533) rtcR/glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
?NC_000913 3583428 = NA (NA)noncoding (1/768 nt) IS1 repeat region

TTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGG  >  NC_000913/3583201‑3583670
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
ttCTTTTTAAGAATCAACCTGATTATCTTACTTTTATACGAGCATTGGTTGGATTAGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTGCAGTTAAAAACCTTTTTGCCGTAACTGACTTTTATAGTAATAATGATGATTTCATAAACCCTGATCTACAAGCACGGATAGTGATCGGGTATTATAGACTTTCAATATTTACTTATGACATTAAAGGTGAtgctgcaaccgtactgctt                                                                                                                                                                                                                             >  2:159445/1‑238 (MQ=255)
   tttttAAGAAGCAACCTGAATATCTTACTTTTTTAAGAGCAATGGATGGATTCGACGTTAATGGATTACGATTATTTAGCCTCTCGAATACAGAACCTTCAGTTAAAAAATTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTACAAAAACCATGATCTACAATAACGGATAGAGATCGGGGATTATAGCATTTAAATATTTACATATGACATTAAGGGTGATGCAGCCAACTTACTAAttt                                                                                                                                                                                                                            >  1:12667/1‑249 (MQ=255)
    ttttAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGATCCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAg                                                                                                                                                                                                                          <  1:276167/250‑1 (MQ=255)
                    gATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTAGAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATGTACTTATGACATTAAAGGTGATGCTGCCATCTTACTGATTTAGTGTATGATGGTGttttt                                                                                                                                                                                                         <  2:210219/251‑1 (MQ=255)
                       tATCTTACTTTTTTAAGGGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGTATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTg                                                                                                                                                                                                        >  1:205842/1‑249 (MQ=255)
                            tACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGc                                                                                                                                                                                                  <  1:129769/250‑1 (MQ=255)
                                          cAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATTATGGTGTTTTTGAGGTGCTCCAGTGGCGTCTGt                                                                                                                                                                                   <  1:96585/251‑1 (MQ=255)
                                                  ggatTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATc                                                                                                                                                                            <  1:64593/250‑1 (MQ=255)
                                                                tGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGt                                                                                                                                                             >  1:322059/1‑251 (MQ=255)
                                                                                                                                  aTTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGAGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATATCTGctct                                                                                           >  1:209468/1‑251 (MQ=255)
                                                                                                                                            aataatGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGt                                                                                  >  1:267963/1‑250 (MQ=255)
                                                                                                                                                   atgatTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATg                                                                          <  1:53740/251‑1 (MQ=255)
                                                                                                                                                     gatTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCCATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTACTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCCCTGCCGGAAAACATGGc                                                                        >  2:201237/1‑251 (MQ=255)
                                                                                                                                                               aCCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGtt                                                              >  1:204940/1‑251 (MQ=255)
                                                                                                                                                                                  gTTAGGGATTGGGGATTATAGCATTTCAATATTTACGTATGTAATTAATGGTGCTGCTGCCAACTGATTGATTTAGTGTATGAGGGTGTTTTTGAGGTGCGCCAGTGCCTTCTGTTTCTACCAGCTGTCCCTCCTGTTCAGCTATTGACTGGGTGGTGCGTAACGGCAAAAGCACCGCAGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACTCCGCTTCGCaa                                           <  2:267963/251‑1 (MQ=14)
                                                                                                                                                                                                  tataGCAATTAAATATTTACTTATGTAAATAAAGGTGATGATGCCAAATTAATGATTTAGTGTATAAGGGTGTTTTAGAGGGGATCCAGTGGCATCTGTTTCTATCAGCGGTCACTCCTGATCAGATACTGAAGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTTACTGCCGTAAAACATGGCAACTGCAGTTCAATTACACCGCTTCTCAACCCGGTACGCACCAg                            <  2:274543/250‑1 (MQ=11)
                                                                                                                                                                                                        ctttCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTATTTTATGATGGTGTTTTTGAGGTGCTCCAGTAGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGCGGTGTGTAACGGCAAACACACCGCCGGACATCAGCGCTTTCTCTGCTCTCAGTGCCGTAACACACGGCATCTGCAGTTCACTTACACACCTTCTAAACCCGGTACGCACCAGaaattc                      >  2:243840/2‑247 (MQ=14)
                                                                                                                                                                                                          ttCAATATTTACTTATGACATTAAAGGTGATGCTGACAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTCAAACATGGCAACAGCAGTTCACTTACACCGCTTCTCAACCCGGGAGGCACCAAAAAATcgtt                   >  2:123477/1‑248 (MQ=14)
                                                                                                                                                                                                                           aCATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATgg  >  1:281614/1‑251 (MQ=32)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
TTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGG  >  NC_000913/3583201‑3583670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.