Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,185,790	IS2 (–) +5 bp	intergenic (‑196/‑50)	potA ← / → pepT	spermidine preferential ABC transporter ATP binding subunit/peptidase T

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	381260 =	NA (NA)	22 (0.760)	20/484	0.4	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	1185790 =	0 (0.000)					intergenic (‑196/‑54)	potA/pepT	spermidine preferential ABC transporter ATP binding subunit/peptidase T
*	?	NC_000913	= 1185794	0 (0.000)	19 (0.660)	17/482	0.6	100%	intergenic (‑200/‑50)	potA/pepT	spermidine preferential ABC transporter ATP binding subunit/peptidase T
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGT  >  NC_000913/381030‑381500                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAAtctc                                                                                                                                                                                                                              >  1:39553/1‑251 (MQ=255)                          gACACGGGCTGTTCATCCCGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATa                                                                                                                                                                                                      >  2:28556/1‑250 (MQ=255)                                 gCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAg                                                                                                                                                                                              <  1:28556/251‑1 (MQ=255)                                       cATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTa                                                                                                                                                                                         >  1:43028/1‑250 (MQ=255)                                             gacgattcttACTGGTACGTACGTGTGCGCCTAAATATGCCTGGTTTGCAACTACCTAGTCGGCAATAAAACATTGCGTACCAAGCGTTGCCTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAg                                                                                                                                                                                  <  2:3461/243‑1 (MQ=38)                                                    atTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGACATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCACTAGCACTTAAAAAAGTGATATTCTTAATACTAGttttt                                                                                                                                                                             >  2:280711/1‑249 (MQ=255)                                                          ggTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGt                                                                                                                                                                     <  1:9316/251‑1 (MQ=255)                                                             acgtacCTGTGCACCTAAATATTCCGTGTTGGCAACTCCCGACTCGGCAATAAAACATCGCCGACCCATCCTTGCTTCCGCTTCAATAGCCGCATTTTTACTCGCTGTGGCAAAACCAATTTTTACGCTATTGCTGATTATTGTGTCAGGATGCATAATCGCATAATTACTAGACGGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAAGAAGTGATAGTCTTCATTCTAGTGTTTAGACTAGTCa                                                                                                                                                                   <  2:279676/250‑1 (MQ=19)                                                                     gtgCGCCTAAATATGCCTGGTTTGCAACTACCGATTCGGCAATAAAACATTGCGGCCCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CACCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAAc                                                                                                                                                          <  2:73414/251‑1 (MQ=255)                                                                       gcacatAAATATGCCTTGATTGTAACTACCTAGTCGGCAAGAAAACATTGCGGTCCAATCATTGATTCCGCTTCAATATCCGCATTTTTAATCGCGGTGGAAAAACCAATTTTTACTCCATTGCTGATTATTGTGCCAGGACGAATCAACGCATAATTACTAGACTGG‑TCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTCATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAg                                                                                                                                                        <  2:297820/246‑1 (MQ=25)                                                                             aaaTATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATATCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgatt                                                                                                                                                  >  2:132524/1‑251 (MQ=255)                                                                                 atGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATg                                                                                                                                              >  1:330723/1‑251 (MQ=255)                                                                                             cAACCACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCCTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAAATACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATTATTGATGTCTTAGGGCCg                                                                                                                                   <  2:149122/250‑1 (MQ=255)                                                                                                                        gCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATATCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAgg                                                                                                       <  1:132524/251‑1 (MQ=255)                                                                                                                                aTCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATc                                                                                               <  1:5551/251‑1 (MQ=255)                                                                                                                                        ttCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGt                                                                                       >  1:15700/1‑251 (MQ=255)                                                                                                                                          ccGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATGTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATGACTAGACTGG‑CCCCCTGAATCGCCAGACAGCCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGtg                                                                                      <  1:140988/250‑2 (MQ=255)                                                                                                                                          ccGCTTAAATAACCGCATTTTTAATCTCGGTTGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTTG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTATTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGtt                                                                                      <  2:43028/250‑1 (MQ=255)                                                                                                                                                cAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCaga                                                                               <  1:280711/251‑1 (MQ=255)                                                                                                                                                          aTTTTTAAGCGCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAAcc                                                                     <  2:15700/251‑1 (MQ=255)                                                                                                                                                                     cGGTGGAAAAACTAATTTTTAAGCCATTGCTGAATAATGTGACAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATAATTTAAATAGATGATAGTCTTAATACTAGTTTTTAGACTAGTAATTGTAGAACAGATGATTGATGTCTTAGGGCAGGAGAAAGGCAGACGGCGTACCACACAGGAAACGATCGTAATTGTTCAGCAGAGCTTTAAACCGGGGATGACgg                                                          >  2:194686/1‑251 (MQ=17)                                                                                                                                                                                  aaTTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGc                                             >  1:135590/1‑251 (MQ=255)                                                                                                                                                                                                    gATTAGTGTGCCAGGACGAAAAAACGCAAAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGAGGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAACAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTagc                           <  2:134123/251‑1 (MQ=255)                                                                                                                                                                                                                          aaCGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCACTCGTTGACAGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTgg     >  1:52620/1‑251 (MQ=21)                                                                                                                                                                                                                           aCGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGc    >  1:152955/1‑251 (MQ=33)                                                                                                                                                                                                                             gCATAATTACTAGA‑TGGTCCCCCTGAATCTCCAGACAACCAATAGCTCTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATGTCTCTGGCGt  <  2:132496/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAACGCATAATTACTAGACTGG‑CCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGT  >  NC_000913/381030‑381500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.