Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,542,042	IS1 (+) +8 bp	coding (6‑13/597 nt)	fimE →	regulator for fimA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3583428 =	NA (NA)	5 (0.080)	5/478	4.7	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 4542049	0 (0.000)					coding (13/597 nt)	fimE	regulator for fimA
Rejected: Coverage evenness skew score above cutoff.
*	?	NC_000913	= 3584195	NA (NA)	82 (1.250)	72/480	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	4542042 =	0 (0.000)					coding (6/597 nt)	fimE	regulator for fimA

ATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTT  >  NC_000913/3583336‑3583494                                                                                             |                                                                   ataGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGtt  >  2:611641/1‑159 (MQ=255) ataGAAATAATGATGATTTCATAAACCCTGAACTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATGTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGtt  <  1:611641/159‑1 (MQ=255)                                                                                             |                                                                   ATAGAAATAATGATGATTTCATAAACCCTGATCTACAAGAACGGTTAGTGATCGGGGATTATAGCATTTCAATATTTACTTATGACATTAAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTT  >  NC_000913/3583336‑3583494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.