Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,743,496	IS5 (–) +4 bp	coding (40‑43/1374 nt)	mdtK →	multidrug efflux pump MdtK

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1743496 =	0 (0.000)	26 (0.830)	26/486	0.3	100%	coding (40/1374 nt)	mdtK	multidrug efflux pump MdtK
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1743499	0 (0.000)	18 (0.570)	16/486	0.7	100%	coding (43/1374 nt)	mdtK	multidrug efflux pump MdtK
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

CCTGCCAGCCAGAAACCTTGTCGCACCTGATGCGCAATGCGCTCACGTCGACCGGAACCATTTAATTGCGCGATAACCGGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1743743‑1743496 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGA  <  NC_000913/2290113‑2289873                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           CCTGCCAGCCAGAAACCTTGTCGCACCTGATGCGCAATGCGCTCACGTCGACCGGAACCATTTAATTGCGCGATAACCGGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGG                                                                                                                                                                                                                                                 >  2:125378/1‑250           AGAAACCTTGTCGCACCTGATGCGCAATGCGCTCACGTCGACCGGAACCATTTAATTGCGCGATAACCGGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAAC                                                                                                                                                                                                                                      >  1:30612/1‑251            GAAACCTTGTCGCACCTGATGCGCAATGCGCTCACGTCGACCGGAACCATTTACTTGCGCGATAATCGGAGTTAATGCCAGCAGCAGTCCGTGCACAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGGCGGTGGCACTATAGCCGCCCGCCATCACGGTATAGACACAACCCATCGCAGTTTGGGCGTTTTGCGCGAGAATCACCGGGATTGCTACGGAAGGTGCGAAC                                                                                                                                                                                                                                      >  2:305734/1‑250               ACCTTGTCGCACCTGATGCGCACTGCGCTCACGTCGACCGGAACCATTTAATTGCGCGATAACCGGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGGGCGAACAAGT                                                                                                                                                                                                                                  >  1:258703/1‑251                             GATGCGCAATGCGCTCACGTCGACCGGAACCATTTAATTGCGCGATAACCGGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAG                                                                                                                                                                                                                      <  1:76231/249‑1                                     ATGCGCTAACGTCGACCGGAACCATTTAATTGCGCGATAACCGGCGTTAATGCCAGCAGCCGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGAACGCCGCCATGTAGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACGGGGATTGCTAAGGAAGGAGCGAACAAGTCCCTGATATGAGATCATGTTTG                                                                                                                                                                                                            >  2:211830/1‑251                                      TGCGCTCACGTCGACCGGTACCATTTAATTGCGCGATAACCGGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGT                                                                                                                                                                                                           >  2:253920/1‑251                                         cCTCACGTCGACCGGAACCATTTAATTGCGCGATACCCGGCTTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCAT                                                                                                                                                                                                        <  1:241865/250‑1                                            CACGTCGACCGGAACCATTTAATTGCGCGATAACCGGCGTTAAGGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTG                                                                                                                                                                                                     <  1:127567/251‑1                                                      GGAACCATTTACTTGCGCGATAACCGGCGCTAATGCCAGCCGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCCTGTCGGTGGCACTATAGCCGCCCGCCATAACGGTATCGACCAAACCCATCGCAGTTTGGGCGATTTGCGCGCGAATCACCGGGATTGCTAAGGAAGGAGCGAACAAGTCCCTGCTATGAGATCATGTTTGTCATCTGGAGCCAT                                                                                                                                                                                              >  2:280556/1‑248                                                                 ATTGCGCGATAACCGGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCAT                                                                                                                                                                                >  1:179211/1‑251                                                                               GGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAAC                                                                                                                                                                  >  2:70299/1‑251                                                                                          CAGCAGCAGACCGTGCCCAAAGAGGATCGCCGGAAGCCAGATAGAAGTCCCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCC                                                                                                                                                       <  2:103545/251‑1                                                                                                           CAAAGAGGATCGCCGGAAGCCATATAGAATTCCCGATAGCGACCGCCGCCATGTAGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGTAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCTTCAACTTAACTTCGCCGACAGTGAATTCAGCAG                                                                                                                                      <  1:270957/251‑1                                                                                                                AGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTAAGCAGTAAGC                                                                                                                                 >  2:281420/1‑251                                                                                                                                           CCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCT                                                                                                      >  2:78451/1‑251                                                                                                                                                           CCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCA                                                                                      <  2:179211/251‑1                                                                                                                                                                             AGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAA                                                                     >  1:303694/1‑250                                                                                                                                                                                          CACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGT                                                        <  2:3716/250‑1                                                                                                                                                                                                      AAAACCCATCGCAGTTTGTGCGAATTGCACGAGAATCACCGGGATTGCTAAGGCAGGAGCGACCAAGTCCATGATATGAGATCATGTTTGTAATCTGGAGCCATAGAACAGGGTTAATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCTGCAGTAAGAGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCCTGGAGCAGATTCTGCAATGGCAAAACATGGTGGAAGTCATCATGCCGTTT                                           >  2:201866/1‑251                                                                                                                                                                                                                   GTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTACCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGCGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTG                              >  2:292691/1‑251                                                                                                                                                                                                                          CGATTTGCGCGAGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCAGCGAGCCGTTGTGCCCCAAGGCTTGTAATGG                       <  1:253920/251‑1                                                                                                                                                                                                                                     AGAATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTT            >  1:283110/1‑251                                                                                                                                                                                                                                        ATCACCGGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATC         >  1:27314/1‑251                                                                                                                                                                                                                                              GGGATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAGGCGCCGTCAGACCAGAAAGGAGATTTTCTTGTCCCGCAGGGAGAAGTTTCTGCCATGGCAAAACATGGGGGAGGTCATCGAGCCGTTTTACCCCAAGGTTGGAAAGGGCCGGCGACCTTATCCGCTGG   >  2:196436/1‑251                                                                                                                                                                                                                                                GATTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGAGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGCCCTTATCCGCTGGA  >  2:243179/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           CCTGCCAGCCAGAAACCTTGTCGCACCTGATGCGCAATGCGCTCACGTCGACCGGAACCATTTAATTGCGCGATAACCGGCGTTAATGCCAGCAGCAGTCCGTGACCAAAGAGGATCGCCGGAAGCCAGATAGAAGTACCGATAGCGACCGCCGCCATGTCGGTGGCACTATAGCCGCCCGCCATCACGGTATCGACAAAACCCATCGCAGTTTGGGCGATTTGCGCGAGAATCACCGGGATTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1743743‑1743496 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGA  <  NC_000913/2290113‑2289873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.